留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

锌离子对活性污泥微生物DNA序列多样性的影响

谢 冰 徐亚同

谢 冰, 徐亚同. 锌离子对活性污泥微生物DNA序列多样性的影响[J]. 环境科学研究, 2003, 16(4): 18-23.
引用本文: 谢 冰, 徐亚同. 锌离子对活性污泥微生物DNA序列多样性的影响[J]. 环境科学研究, 2003, 16(4): 18-23.
XIE Bing, XU Ya-tong. The Effect of Zinc Ions on Activated Sludge Microbes and Its DNA Sequence Diversity Analysis[J]. Research of Environmental Sciences, 2003, 16(4): 18-23.
Citation: XIE Bing, XU Ya-tong. The Effect of Zinc Ions on Activated Sludge Microbes and Its DNA Sequence Diversity Analysis[J]. Research of Environmental Sciences, 2003, 16(4): 18-23.

锌离子对活性污泥微生物DNA序列多样性的影响

基金项目: 上海市科委项目(02JG05030);上海市生态学重点学科资助项目

The Effect of Zinc Ions on Activated Sludge Microbes and Its DNA Sequence Diversity Analysis

  • 摘要: 研究了锌离子对活性污泥微生物群落结构的影响.不同浓度的锌离子对有机物的降解和氮的转化均有抑制.经过驯化后,处理系统对有机物的降解可以逐步得到恢复,而对于氨氮和亚硝酸氮的降解则并不随着驯化时间的延长而得到改善.随机扩增多态性RAPD分析表明,在不同的驯化阶段,DNA序列的分子多样性是不同的.结果显示,加入锌离子的微生物多样性明显小于对照组,驯化成熟的实验组尽管在COD的去除率上和对照组没有什么差别,但在生物多样性、丰富度、均匀度和相似性上与驯化初期有较大的差别.从RAPD条带上可清楚地看到某些细菌的消失,以及耐受锌离子的种类的持续存活.

     

  • [1] Poon C P C, Bhayani K H. Metal toxicity to sewage organisms[J]. J Snit Engng Div Am Soc,civ Engrs, 1971,79:161-169.
    [2] Lamb A, Tollefson E L. Toxic effects of cupric, chromate and chromic ions on biological oxidation[J]. Water Res, 1973,7:599-613.
    [3] Mowat A. Measurement of metal toxicity by biochemical oxygen demand[J].J Water Pollut Control Fed, 1976,48:853-866.
    [4] Jerzy Mazierski. Effect of chromium on the growth rate of activated sludge bacterial[J]. Water Res, 1994,29:1479-1482.
    [5] Blok J. Disturbance of biological wastewater treatment by toxic industrial waste [J]. Prog, Water Technol, 1976,8 (17): 180.
    [6] Vankova S, Kupec J, Hoffmann J. Toxicity of chromium to activated sludge[J]. Ectoxic Environ Safety, 1999,42:16-21.
    [7] Madoni P, Davoli D, Guglielmi L. Response of SOUR and AUR to heavy metal contamination in activated sludge[J]. Water Res, 1999, 33 (10):2459-2464.
    [8] Barberio C, Fani R. Biodiversity of an Acinetobacter population isolated from activated sludge [J]. Res Microbiol, 1998,149: 665-673.
    [9] Yah H J, Si L D, Nai H W. RAPD analysis of natural population of Acanthopanax brachypus [J]. Cell Research, 1997,7: 99-106.
    [10] Williams J G, Kubeli K A R, Livak K J, et al. DNA phlymophisms amplified by arbitrary primers are useful as genetic markers[J]. Nucleic Acids Research, 1990,18:6531-6535.
    [11] Welsh J, Petersen C, McClelland M. Fingerprinting genomes using PCR arbitrary primers [J]. Nucleic Acids Research, 1990,18: 7213-7218.
    [12] Schierwater B, Ender A. Different thermostable DNA ploymerases may amplify different RAPD products[J]. Nucleic Acids Research, 1993,20:4647-4648.
    [13] Hardy H, Balick M, Schierwater B. Application of random amplified poly morphic DNA (RAPD) in molecular ecology [J]. Molecular Ecology,1992,1:55-63.
    [14] Thormann C E, Ferreira M E, Camargo L E A, et al. Comparison of RFLP and RAPD markers to estimating genetic relationships within and among crucifernus species[J]. Theor Appl Genet, 1994,88:973-980.
    [15] Xie B, Kang K S, Nakamura E,et al. The effect of heavy metals on activated sludge process and its microbial community analysis using 16S Ribosomal DNA [J]. International Journal of Environment and pollution,2003,18: 571-586.
    [16] APHA. Standard methods for examination of water and wastewater(15th edu) [M]. Washington DC: APHA (American Public Health Association), AWWA(American Water Works Association), WPCF(Water Pollution Control Federation), 1980.
    [17] Zhou J, Bruns M A, Tiedje J M. DNA recovery from soil of diverse composition[J]. Appl Environ Microbiol, 1996,62: 316-322.
    [18] Sokal R R,Sneath P H A.The Principle of numerical taxonomy[M].USA: San Fanciseo W. H. Freeman and Company, 1963.
    [19] Wan B, Xu W W, Wang J Z, et al. Tagging and mapping rice thermo-sensitive genic male sterile gene using molecular markers [J]. Theor Appl Genet,1995,91:1111-1114.
  • 加载中
计量
  • 文章访问数:  1342
  • HTML全文浏览量:  13
  • PDF下载量:  1504
  • 被引次数: 0
出版历程
  • 收稿日期:  2003-01-16
  • 刊出日期:  2003-08-25

目录

    /

    返回文章
    返回