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苯并[a]芘对鲫鱼生物标志物的影响研究

李 康 周忠良 陈立侨 孟艳玲 吴 鹰

李 康, 周忠良, 陈立侨, 孟艳玲, 吴 鹰. 苯并[a]芘对鲫鱼生物标志物的影响研究[J]. 环境科学研究, 2006, 19(1): 91-95.
引用本文: 李 康, 周忠良, 陈立侨, 孟艳玲, 吴 鹰. 苯并[a]芘对鲫鱼生物标志物的影响研究[J]. 环境科学研究, 2006, 19(1): 91-95.
LI Kang, ZHOU Zhong-liang, CHEN Li-qiao, MENG Yan-ling, WU Ying. Assessment of Various Biomarkers in Carassius auratus Exposed to Benzo[a]pyrene[J]. Research of Environmental Sciences, 2006, 19(1): 91-95.
Citation: LI Kang, ZHOU Zhong-liang, CHEN Li-qiao, MENG Yan-ling, WU Ying. Assessment of Various Biomarkers in Carassius auratus Exposed to Benzo[a]pyrene[J]. Research of Environmental Sciences, 2006, 19(1): 91-95.

苯并[a]芘对鲫鱼生物标志物的影响研究

基金项目: 上海市科委重大项目(002312015);华东师范大学2004年优秀博士培养基金(BYJ-02);国家自然科学基金资助项目(30028018)

Assessment of Various Biomarkers in Carassius auratus Exposed to Benzo[a]pyrene

  • 摘要: 研究了苯并[a]芘暴露对鲫鱼(Carassius auratus)的几种生物标志物的影响.在鲫鱼接受3个不同质量分数苯并[a]芘(BaP)的腹腔注射14 d后,分析了其肝脏7-乙氧基-3-异吩噁唑酮-脱乙基酶(EROD)活性,以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)等抗氧化酶活性和红细胞核异常(NA)率变化.结果显示:w(BaP)为1 mg/kg处理组的EROD活性显著升高(P<0.001).w(BaP)为0.1和1 mg/kg处理组SOD活性显著低于对照组(P<0.001),在暴露结束时,w(BaP)为1 mg/h处理组CAT活性显著低于对照组(P<0.01),而w(BaP)为0.1和1 mg/kg处理组GPx活性被显著抑制(P<0.001).表明BaP对EROD活性有诱导作用,而对SOD,CAT和GPx等抗氧化酶活性的抑制作用明显.所有处理组NA率与对照组均无显著差异.因此,鲫鱼EROD,SOD,CAT和GPx等酶活性的改变适于作为生物标志物,可以用来显示暴露于多环芳烃有机污染物鱼类的生活状态.

     

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出版历程
  • 收稿日期:  2004-12-20
  • 修回日期:  2006-01-30
  • 刊出日期:  2006-02-25

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