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培养基种类和培养条件对白腐真菌生长和产酶特性的影响

王 灿 胡洪营 于 茵 文湘华

王 灿, 胡洪营, 于 茵, 文湘华. 培养基种类和培养条件对白腐真菌生长和产酶特性的影响[J]. 环境科学研究, 2007, 20(2): 9-13.
引用本文: 王 灿, 胡洪营, 于 茵, 文湘华. 培养基种类和培养条件对白腐真菌生长和产酶特性的影响[J]. 环境科学研究, 2007, 20(2): 9-13.
WANG Can, HU Hong-ying, YU Yin, WEN Xiang_hua. Influence of Culturing Mediums and Conditions on Characterization of Growth and Enzyme Production of White Rot Fungi[J]. Research of Environmental Sciences, 2007, 20(2): 9-13.
Citation: WANG Can, HU Hong-ying, YU Yin, WEN Xiang_hua. Influence of Culturing Mediums and Conditions on Characterization of Growth and Enzyme Production of White Rot Fungi[J]. Research of Environmental Sciences, 2007, 20(2): 9-13.

培养基种类和培养条件对白腐真菌生长和产酶特性的影响

基金项目: 国家高技术研究发展计划 (863)项目(2004AA601061)

Influence of Culturing Mediums and Conditions on Characterization of Growth and Enzyme Production of White Rot Fungi

  • 摘要: 以白腐真菌(Phanerochaete chrysosporium)为研究对象,比较了该菌种在人工合成培养基和天然培养基中的生长和产酶特性,考察了竹子浸出液,pH,载体和抗生素等对白腐真菌在天然培养基中生长和产酶特性的影响.结果表明,天然培养基中白腐真菌的生物量、菌丝小球直径和木质素过氧化物酶(LiP)活性均大于人工培养基.以天然培养基为基础培养基,加入竹子浸出液可以促进白腐真菌的生长和提高木质素过氧化物酶活性;较低pH(4.5)条件下菌丝小球直径较小;载体的加入使得菌体以附着形式生长;抗生素两性霉素B对白腐真菌的生长和产酶的影响存在阈值,当ρ(两性霉素B)超过50 mg/L时,白腐真菌的生长和产酶受到明显的抑制.

     

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  • 刊出日期:  2007-04-25

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