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通用引物PCR方法在地表水病原菌检测中的应用

刘永军 张崇淼 王晓昌 孙 茵 薛小平

刘永军, 张崇淼, 王晓昌, 孙 茵, 薛小平. 通用引物PCR方法在地表水病原菌检测中的应用[J]. 环境科学研究, 2007, 20(2): 89-93.
引用本文: 刘永军, 张崇淼, 王晓昌, 孙 茵, 薛小平. 通用引物PCR方法在地表水病原菌检测中的应用[J]. 环境科学研究, 2007, 20(2): 89-93.
LIU Yong-jun, ZHANG Chong-miao, WANG Xiao-chang, SUN Yin, XUE Xiao-ping. Application of Pathogenic Bacteria Detection in Surface Water by Universal Primer PCR Method[J]. Research of Environmental Sciences, 2007, 20(2): 89-93.
Citation: LIU Yong-jun, ZHANG Chong-miao, WANG Xiao-chang, SUN Yin, XUE Xiao-ping. Application of Pathogenic Bacteria Detection in Surface Water by Universal Primer PCR Method[J]. Research of Environmental Sciences, 2007, 20(2): 89-93.

通用引物PCR方法在地表水病原菌检测中的应用

基金项目: 国家自然科学基金重大国际合作项目(50621140001); 国家自然科学基金资助项目(50478048)

Application of Pathogenic Bacteria Detection in Surface Water by Universal Primer PCR Method

  • 摘要: 为了探索通用引物PCR方法在地表水病原细菌检测中的应用价值,利用16S rRNA基因的高度保守性,设计并合成细菌的通用引物,采用合成的引物扩增参考菌株及西安市区不同地表水样,并对PCR产物分别进行序列测定及序列同源性分析,同时检测水样中的细菌总数和粪大肠杆菌浓度.结果显示,通用引物扩增4种参考菌株,320 bp处均可得到清晰的电泳条带,其特异性通过序列测定及同源性分析得到进一步验证;通用引物PCR可检出250 ng/L的埃希氏大肠杆菌标准菌株DNA,其PCR检测的灵敏度可达0.275 CFU/mL;用通用引物对西安市区不同地表水样进行PCR扩增,其中■河、兴庆湖、大唐芙蓉园北湖、北石桥污水处理厂出水样品320 bp处都得到了清晰的电泳条带,而黑河水样没有条带;与之相对应的粪大肠杆菌群检测结果显示,只有北石桥污水处理厂出水和兴庆湖水样有粪大肠杆菌检出.

     

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  • 刊出日期:  2007-04-25

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