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处理含酚废水曝气生物流化床中丝状菌种群结构的解析

董 婧 宋玉栋 周岳溪 杨婧晖 杨 健

董 婧, 宋玉栋, 周岳溪, 杨婧晖, 杨 健. 处理含酚废水曝气生物流化床中丝状菌种群结构的解析[J]. 环境科学研究, 2010, 23(8): 1068-1075.
引用本文: 董 婧, 宋玉栋, 周岳溪, 杨婧晖, 杨 健. 处理含酚废水曝气生物流化床中丝状菌种群结构的解析[J]. 环境科学研究, 2010, 23(8): 1068-1075.
DONG Jing, SONG Yu-dong, ZHOU Yue-xi, YANG Jing-hui, YANG Jian. Analysis of the Structure of Filamentous Microbial Groups from Aerated Biological Fluidized Tank Treating Phenolic Wastewater[J]. Research of Environmental Sciences, 2010, 23(8): 1068-1075.
Citation: DONG Jing, SONG Yu-dong, ZHOU Yue-xi, YANG Jing-hui, YANG Jian. Analysis of the Structure of Filamentous Microbial Groups from Aerated Biological Fluidized Tank Treating Phenolic Wastewater[J]. Research of Environmental Sciences, 2010, 23(8): 1068-1075.

处理含酚废水曝气生物流化床中丝状菌种群结构的解析

基金项目: 国家水体污染控制与治理科技重大专项(2008ZX07207-004)

Analysis of the Structure of Filamentous Microbial Groups from Aerated Biological Fluidized Tank Treating Phenolic Wastewater

  • 摘要: 应用曝气生物流化床反应器(ABFT)处理含酚废水,随着进水ρ(苯酚)由100 mg/L提高到400 mg/L,苯酚的去除率稳定在100%左右,且苯酚的去除主要发生在4个反应池中的第一池. 利用扫描电镜(SEM)、荧光原位杂交(FISH)和变性梯度凝胶电泳(DGGE)技术对反应器内微生物种群进行分析. 扫描电镜结果表明,随着反应器进水ρ(苯酚)的提高,填料生物膜微生物逐渐演变为以丝状菌为主. 针对SSU rRNA的荧光原位杂交结果表明,反应器内丝状微生物主要为真菌. 分别对细菌和真菌的16S rDNA和18S rDNA 进行PCR-DGGE分析,结果表明,反应器内主要的苯酚降解菌为克氏地霉(Geotrichum klebahnii),同时存在发硫菌等丝状细菌. 聚类及多样性分析表明,苯酚对微生物种群表现出很强的选择性.

     

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出版历程
  • 收稿日期:  2010-01-06
  • 修回日期:  2010-02-24
  • 刊出日期:  2010-08-25

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