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蛋白核小球藻(Chlorella pyrenoidosa)微板毒性分析方法优化

袁 静 刘树深 王丽娟 邵玉敏

袁 静, 刘树深, 王丽娟, 邵玉敏. 蛋白核小球藻(Chlorella pyrenoidosa)微板毒性分析方法优化[J]. 环境科学研究, 2011, 24(5): 553-558.
引用本文: 袁 静, 刘树深, 王丽娟, 邵玉敏. 蛋白核小球藻(Chlorella pyrenoidosa)微板毒性分析方法优化[J]. 环境科学研究, 2011, 24(5): 553-558.
YUAN Jing, LIU Shu-shen, WANG Li-juan, SHAO Yu-min. Optimization of Microplate Toxicity Analysis Method Based on Chlorella Pyrenoidose[J]. Research of Environmental Sciences, 2011, 24(5): 553-558.
Citation: YUAN Jing, LIU Shu-shen, WANG Li-juan, SHAO Yu-min. Optimization of Microplate Toxicity Analysis Method Based on Chlorella Pyrenoidose[J]. Research of Environmental Sciences, 2011, 24(5): 553-558.

蛋白核小球藻(Chlorella pyrenoidosa)微板毒性分析方法优化

基金项目: 国家自然科学基金项目(20977065);长江水环境实验室自主课题(YRWEY1002)

Optimization of Microplate Toxicity Analysis Method Based on Chlorella Pyrenoidose

  • 摘要: 以蛋白核小球藻(Chlorella pyrenoidosa,CP)为指示生物,96孔微板为暴露载体,污染物对藻的72 h生长抑制率为毒性指标,通过系统地检测蛋白核小球藻的生长曲线和吸收光谱,确定藻细胞密度和683 nm波长处光密度(D683)之间的线性关系,考察不同初始藻密度、照度、暴露时间和暴露体积对藻生长的影响,建立了蛋白核小球藻微板毒性分析方法(CP-MTA法).将CP-MTA法应用于重金属盐、除草剂、杀虫剂以及离子液体等8种化学品对蛋白核小球藻的生长抑制毒性测试,以pEC50为毒性指标,毒性大小顺序为敌草快>CuSO4·5H2O≈CdCl2·2.5H2O>氯化1-甲基-3-辛基咪唑([Omim]Cl)>草甘膦>氯化1-甲基-3-丁基咪唑([Bmim]Cl)>敌敌畏>乐果,与文献结果一致.CP-MTA法由于以微板为反应载体,所需样品少,便于多次平行,数据重复性好.

     

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出版历程
  • 收稿日期:  2010-09-11
  • 修回日期:  2011-02-08
  • 刊出日期:  2011-05-25

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