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壬基酚对中国林蛙精巢中StAR表达的影响

李忻怡 王 慧 张育辉

李忻怡, 王 慧, 张育辉. 壬基酚对中国林蛙精巢中StAR表达的影响[J]. 环境科学研究, 2011, 24(5): 564-570.
引用本文: 李忻怡, 王 慧, 张育辉. 壬基酚对中国林蛙精巢中StAR表达的影响[J]. 环境科学研究, 2011, 24(5): 564-570.
LI Xin-yi, WANG Hui, ZHANG Yu-hui. Effects of Nonylphenol on StAR Expression in the Testes of Frog Rana Chensinensis[J]. Research of Environmental Sciences, 2011, 24(5): 564-570.
Citation: LI Xin-yi, WANG Hui, ZHANG Yu-hui. Effects of Nonylphenol on StAR Expression in the Testes of Frog Rana Chensinensis[J]. Research of Environmental Sciences, 2011, 24(5): 564-570.

壬基酚对中国林蛙精巢中StAR表达的影响

基金项目: 国家自然科学基金项目(30770243);陕西省自然科学基金项目(SJ08C101)

Effects of Nonylphenol on StAR Expression in the Testes of Frog Rana Chensinensis

  • 摘要: 为研究水体壬基酚(Nonylphenol,NP)污染对两栖动物精巢中类固醇生成急性调节蛋白(StAR)的影响,将雄性成体中国林蛙(Rana chensinensis)分别暴露于c(NP)为10-7,10-6和10-5 mol/L的水体中10,20和30 d.取其精巢组织,在成功扩增获得273 bp的StAR cDNA片段后合成探针,用原位杂交方法检测StAR mRNA在精巢的表达,用免疫组织化学方法检测StAR蛋白的表达.结果在对照组和各NP处理组中均检测到StAR mRNA和蛋白的阳性表达,阳性反应主要在精巢的Leydig细胞.StAR表达相对值的检测结果表明,c(NP)为10-5 mol/L 的水体对StAR表达有抑制趋势,c(NP)为10-7和10-6 mol/L 的水体可上调StAR的表达,表明NP对StAR表达由抑制转向诱导的临界浓度在c(NP)为10-6 mol/L与10-5 mol/L 之间.在c(NP)为10-7和10-6 mol/L 处理组,StAR表达值随c(NP)的增高而增加.在c(NP)为10-6 mol/L 处理组,暴露30 d后StAR表达相对值最大,呈现NP对StAR表达具有累积效应.c(NP)为10-7 mol/L对StAR表达不随时间延长显示累积效应.提示一定剂量范围的NP可通过调控StAR的表达干扰动物类固醇激素的合成.

     

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出版历程
  • 收稿日期:  2010-08-13
  • 修回日期:  2010-12-24
  • 刊出日期:  2011-05-25

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