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不同SBR系统N2O排放及微生物群落比较

朴 哲 陈新兵 赵秀锦 刘艳萍 何成达 殷士学

朴 哲, 陈新兵, 赵秀锦, 刘艳萍, 何成达, 殷士学. 不同SBR系统N2O排放及微生物群落比较[J]. 环境科学研究, 2011, 24(6): 698-703.
引用本文: 朴 哲, 陈新兵, 赵秀锦, 刘艳萍, 何成达, 殷士学. 不同SBR系统N2O排放及微生物群落比较[J]. 环境科学研究, 2011, 24(6): 698-703.
PIAO Zhe, CHEN Xin-bing, ZHAO Xiu-jin, LIU Yan-ping, HE Cheng-da, YIN Shi-xue. Comparative Analysis of N2O Emissions and Microbial Communities from Different Sequencing Batch Reactor Systems[J]. Research of Environmental Sciences, 2011, 24(6): 698-703.
Citation: PIAO Zhe, CHEN Xin-bing, ZHAO Xiu-jin, LIU Yan-ping, HE Cheng-da, YIN Shi-xue. Comparative Analysis of N2O Emissions and Microbial Communities from Different Sequencing Batch Reactor Systems[J]. Research of Environmental Sciences, 2011, 24(6): 698-703.

不同SBR系统N2O排放及微生物群落比较

基金项目: 国家自然科学基金项目(22677047);国家水体污染控制与治理科技重大专项(2008ZX07317-007-1)

Comparative Analysis of N2O Emissions and Microbial Communities from Different Sequencing Batch Reactor Systems

  • 摘要: 为了解污水脱氮中微生物群落对N2O排放的影响,在相同的工艺条件下,研究了制药厂(A)和啤酒厂(B)2种不同来源污泥在SBR系统中的N2O排放特性.结果发现:①A和B 2个系统总氮去除率在97.5%和98.6%的情况下,脱氮中N2O态氮所占比例分别为6.35%和2.84%,相差2倍以上.②A系统的N2O排放时期主要集中在好氧硝化段,而B系统则主要集中在缺氧反硝化段.③在1个脱氮周期内,A系统只有1个N2O排放高峰,出现在好氧硝化段(第3小时);而B系统有2个N2O排放高峰,分别出现在好氧硝化段(第3小时)和缺氧反硝化段(第6小时).采用PCR-DGGE技术分析微生物群落特征发现,A系统和B系统的微生物群落有明显差异,表明污水脱氮中微生物群落是影响N2O排放的重要因素.通过优化微生物群落结构,可有效控制污水脱氮中N2O排放.

     

  • [1] IPCC.Climate change: the science of climate change[M].Cambridge:Cambridge University Press,1995:21-24.
    [2] KHALIL M A K,RASMUSSEN R A,SHEARER M J.Atmospheric nitrous oxide: patterns of global change during recent decades and centuries[J].Chemosphere,2002,47:807-821.
    [3] KHALIL M A K,RASMUSSEN R A.The global sources of nitrous oxide [J].Journal of Geophysical Research,1992,97(D13):14651-14660.
    [4] 刘秀红,杨庆,吴昌永,等.不同污水生物脱氮工艺中N2O释放量及影响因素[J].环境科学学报,2006,26(12):1940-1947.
    [5] 阮文权,陈坚.好氧颗粒污泥同步硝化反硝化脱氮过程中N2O的产生[J].无锡轻工大学学报,2004,23(4):37-40,64.
    [6] 吕锡武,稻森悠平,水落元之.同步硝化反硝化脱氮及处理过程中N2O的控制研究[J].东南大学学报:自然科学版,2001,31(1):95-99.
    [7] PARK K Y,LEE J W,INAMORI Y,et al.Effects of fillmodes on N2O emission from the SBR treating domestic wastewater[J].Water Sci Technol,2001,43(3):147-150.
    [8] ZENG R J,LEMAIRE R,YUAN Z,et al.Simultaneous nitrification,denitrification,and phosphorus removal in a lab-scale sequencing batch reactor[J].Biotechnology and Bioengineering,2003,84(2):170-178.
    [9] TAKASHI O,KAZUTAKA K,MICHIHIRO Y.Reducing nitrous oxide gas emissions from fill-and-draw activated sludge process[J].Water Res,1995,29(6):1607-1608.
    [10] SATOSHI T,MAKIO M,YUZURU K,et al. Effect of salinity on nitrous oxide emission in the biological nitrogen removal process for industrial wastewater [J].J Hazard Mater,2005,B119:93-98.
    [11] ITOKAWA H,HANAKI K,MATSUO T.Nitrous oxide production in high-loading biological nitrogen removal process under low COD/N ratio condition [J].Water Res,2001,35(3):657-664.
    [12] KISHIDA N,KIM J H,KIMOCHI Y,et al.Effect of C/N ratio on nitrous oxide emission from swine wastewater treatment process[J].Water Sci Technol,2004,49(5/6):359-365.
    [13] NODA N,KANEKO N,MIKAMI M,et al.Effects of SRT and DO on N2O reductase activity in an anoxicoxic activated sludge system[J].Water Sci Technol,2003,48(11/12):363-370.
    [14] TAKAYA N,CATALAN-SAKAIRI M A B,SAKAGUCHI Y,et al.Aerobic denitrifying bacteria that produce low levels of nitrous oxide[J].Applied and Environmental Microbiology,2003,69(6):3152-3157.
    [15] 尹明锐,汪苹,刘健楠,等.具有N2O控逸能力的异养硝化-好氧反硝化菌株的筛选鉴定[J].环境科学研究,2010,23(4):515-520.
    [16] KOSCHORRECK M,CONRAD R,MOORE E.Oxidation of nitric oxide by a new heterotrophic Pseudomonas sp.[J].Archives of Microbiology,1996,166:23-31.
    [17] INAMORI Y,WU X L,MIZUOCHI M.N2O producing capability of Nitrosomonas europaea,Nitrobacter winogradskyi and Alcaligenesfaecalis[J].Water Sci Technol,1997,36(10):65-72.
    [18] 国家环境保护总局.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002.
    [19] 高平平,赵立平.可用于微生物群落分子生态学研究的活性污泥总DNA提取方法研究[J].生态学报,2002,22(11):2015-2019.
    [20] PIAO Z,YANG L Z,ZHAO L P,et al.Actinobacterial community structure in soils receiving long-term organic and inorganic amendments[J].Appl Environ Microbiol,2008,74(2):526-530.
    [21] MUYZER G,WAAL E C,UITTERLINDEN A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of Polymerase chain reaction-amplified genes coding for 16S rRNA [J].Applied and Environmental Microbiology,1993,59(3):695-700.
    [22] MUYZER G,SMALLA K.Application of denaturing gradient gel electrophoresis(DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology[J].Antoni van Leeuwenhoek,1998,73:127-141.
    [23] 吕锡武.同时硝化反硝化的理论和实践[J].环境化学,2002,21(6):564-570.
    [24] HU Z,ZHANG J,LI S P,et al.Effect of aeration rate on the emission of N2O in anoxic-aerobic sequencing batch reactors (A/O SBRs) [J].Journal of Bioscience and Bioengineering,2010,109(5):487-491.
    [25] KIM S W,MIYAHARA M,FUSHINOBU S,et al. Nitrous oxide emission from nitrifying activated sludge dependent on denitrification by ammonia-oxidizing bacteria[J].Bioresource Technology,2010,101(11):3958-3963.
    [26] YANG Q,LIU X,PENG C,et al.N2O production during nitrogen removal via nitrite from domestic wastewater,main sources and control method[J].Environ Sci Technol,2009,43:9400-9406.
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出版历程
  • 收稿日期:  2010-06-30
  • 修回日期:  2010-12-08
  • 刊出日期:  2011-06-25

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