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猪粪中典型类固醇雌激素的固相萃取/GC-MS检测

刘 敏 岳 波 尹平河 黄泽春 王 琪 黄启飞 何 洁

刘 敏, 岳 波, 尹平河, 黄泽春, 王 琪, 黄启飞, 何 洁. 猪粪中典型类固醇雌激素的固相萃取/GC-MS检测[J]. 环境科学研究, 2011, 24(7): 763-768.
引用本文: 刘 敏, 岳 波, 尹平河, 黄泽春, 王 琪, 黄启飞, 何 洁. 猪粪中典型类固醇雌激素的固相萃取/GC-MS检测[J]. 环境科学研究, 2011, 24(7): 763-768.
LIU Min, YUE Bo, YIN Ping-he, HUANG Ze-chun, WANG Qi, HUANG Qi-fei, HE Jie. Analysis of Typical Steroid Estrogens in Pig Manure by Solid Phase Extraction/GC-MS[J]. Research of Environmental Sciences, 2011, 24(7): 763-768.
Citation: LIU Min, YUE Bo, YIN Ping-he, HUANG Ze-chun, WANG Qi, HUANG Qi-fei, HE Jie. Analysis of Typical Steroid Estrogens in Pig Manure by Solid Phase Extraction/GC-MS[J]. Research of Environmental Sciences, 2011, 24(7): 763-768.

猪粪中典型类固醇雌激素的固相萃取/GC-MS检测

基金项目: 国家环保公益性行业科研专项(200909042)

Analysis of Typical Steroid Estrogens in Pig Manure by Solid Phase Extraction/GC-MS

  • 摘要: 以猪粪中常见且具有较大潜在危害的E1(雌酮),17α-E2(17α-雌二醇),17β-E2(17β-雌二醇)和E3(雌三醇)为研究对象,建立了猪粪中这4种典型类固醇雌激素的GC-MS(气相色谱-质谱)分析检测方法.着重优化了样品的前处理方法,包括对超声提取剂、超声提取时间、固相萃取淋洗剂、衍生化温度和时间的优化选择.结果表明:在优化的试验条件下,猪粪中的典型雌激素E1,17α-E2,17β-E2和E3检出限分别为8.0,2.5,5.1和3.3 μg/kg,平均加标回收率在92.3%~137.4%之间.

     

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出版历程
  • 收稿日期:  2010-10-17
  • 修回日期:  2011-01-07
  • 刊出日期:  2011-07-25

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