留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

一株具砷氧化和反硝化功能的无色杆菌的筛选和鉴定

曾 琳 朱琼芳 卢贯能 陈来琳 匡庐峰 柯 林

曾 琳, 朱琼芳, 卢贯能, 陈来琳, 匡庐峰, 柯 林. 一株具砷氧化和反硝化功能的无色杆菌的筛选和鉴定[J]. 环境科学研究, 2011, 24(10): 1123-1128.
引用本文: 曾 琳, 朱琼芳, 卢贯能, 陈来琳, 匡庐峰, 柯 林. 一株具砷氧化和反硝化功能的无色杆菌的筛选和鉴定[J]. 环境科学研究, 2011, 24(10): 1123-1128.
ZENG Lin, ZHU Qiong-fang, LU Guan-neng, CHEN Lai-lin, KUANG Lu-feng, KE Lin. Screening and Identification of an Achromobacter Strain for Both Arsenic Oxidizing and Denitrifying Abilities[J]. Research of Environmental Sciences, 2011, 24(10): 1123-1128.
Citation: ZENG Lin, ZHU Qiong-fang, LU Guan-neng, CHEN Lai-lin, KUANG Lu-feng, KE Lin. Screening and Identification of an Achromobacter Strain for Both Arsenic Oxidizing and Denitrifying Abilities[J]. Research of Environmental Sciences, 2011, 24(10): 1123-1128.

一株具砷氧化和反硝化功能的无色杆菌的筛选和鉴定

基金项目: 国家自然科学基金项目(21047003);教育部留学回国人员科研启动基金项目(x2hjB7100180)

Screening and Identification of an Achromobacter Strain for Both Arsenic Oxidizing and Denitrifying Abilities

  • 摘要: 利用含As3+肉汤培养基,从广西河池砷污染地区水样和沉积物样中通过多次分离、纯化获取砷耐受菌.进一步从砷耐受菌中筛选出在好氧条件下可以同时进行砷氧化和反硝化的多功能菌株cll-35. 对该菌株进行形态观察,并利用16S rDNA序列分析方法进行鉴定,发现该菌株为革兰氏阴性菌,与Achromobacter denitrificans strain 22426和Achromobacter xylosoxidans strain C8B的同源性均达99%;该菌株在NO3-和As3+同时存在的条件下好氧反硝化能力和砷氧化速率均得到提高;在只含NO3-的条件下,NO3-的去除率为53.65%,而在As3+和NO3-同时存在的条件下,NO3-的去除率为75.27%.在不含NO3-和含NO3-的条件下,As3+的转化率都在99%以上,而在含NO3-的条件下,As3+的氧化速率更快.这种相互促进可能与反硝化过程中的电子传递和砷氧化过程中的动态平衡有关.

     

  • [1] CULLEN W R,REIMER K J.Arsenic speciation in the environment[J].Chemical Reviews,1989,89:713-764.
    [2] NG J C,WANG J,SHRAIM A.A global health problem caused by arsenic from natural sources[J].Chemosphere,2003,52:1353-1359.
    [3] RAVEN K P,JAIN A,LOEPPERT R H.Arsenite and arsenate adsorption on ferrihydrite:kinetics,equilibrium,and adsorption envelopes[J].Environ Sci Technol,1998,32:344-349.
    [4] SMEDLEY P L,KINNIBURGH D G.A review of the source,behavior and distribution of arsenic in natural waters[J].Applied Geochemistry,2002,17:517-568.
    [5] 戴树桂.环境化学[M].北京:高等教育出版社,1996(1999重印):100-105.
    [6] 孙威.叶类蔬菜贮存过程中硝酸盐和亚硝酸盐的含量研究[D].长春:东北师范大学,2006:3-4.
    [7] 国家环境保护总局.水和废水监测方法[M].4版.北京:中国环境科学出版社,2002:258-274.
    [8] 孙静,吴攀,韩志伟,等.高砷煤矿废水对表层岩溶水水质的影响[J].环境科学研究,2009,22(12):1440-1444.
    [9] 姚丽华,陈树茂.钨冶炼过程中含砷含氨氮废水的治理[J].稀有金属与硬质合金,2007,35(3):31-33.
    [10] 郭华明,陈思,任燕.反硝化菌的耐砷驯化及其对水铁矿吸附态砷迁移转化的影响[J].地学前缘,2008,15(5):317-323.
    [11] CHEN X P,ZHU Y G,HONG M N,et al. Effects of different forms of nitrogen fertilizers on arsenic uptake by rice plants[J].Environ Toxicol Chem,2008,27(4):881-887.
    [12] 冯克亮.水质砷污染及除砷新技术[J].海洋环境科学,1994,13(1):78-81.
    [13] 王耀龙,魏云霞,李晓丽,等.废水脱氮技术研究进展[J].环境工程,2010,28:119-123.
    [14] SUN W,SIERRA-ALVAREZ R,MILNER L,et al.Arsenite and ferrous iron oxidation linked to chemolithotrophic denitrification for the immobilization of arsenic in anoxic environments[J].Environ Sci Technol,2009,43:6585-6591.
    [15] BARCO R A,PATIL D G,XU W H,et al.The development of iodide-based methods for batch and on-line determinations of phosphite in aqueous samples[J].Talanta,2006,69:1292-1299.[16]林娜,郭楚玲,柯林,等.富营养化池塘中好氧反硝化菌的分布及脱氮研究[J].中国科技论文在线,2010,5(5):369-372.
    [16] 蔡林.细菌砷解毒基因的鉴定及功能研究[D].武汉:华中农业大学,2009:27.
    [17] GUO H M,YANG S Z,TANG X H,et al.Groundwater geochemistry and its implications for arsenic mobilization in shallow aquifers of the Hetao basin,Inner Mongolia[J].Sci Total Environ,2008,393(1):131-144.
    [18] GUO H M,TANG X H,YANG S Z,et al.Effect of indigenous bacteria on geochemical behaviors of arsenic in aquifer sediment s from the Hetao Basin,Inner Mongolia:evidence from sediment incubations[J].Applied Geochemistry,2008,23(12):3267-3277.
    [19] 孔庆鑫,李君文,王新为,等.一种新的好氧反硝化菌筛选方法的建立及新菌株的发现[J].应用与环境生物学报,2005,11(2):222-225.
    [20] ROBERSON L A,KUENEN J G.Aerobic denitrification:a controversy revived[J].Arch Microbiol,1984,139:351-354.
    [21] CAI L,LIU G H,RENSING C, et al.Genes involved in arsenic transformation and resistance associated with different levels of arsenic-contaminated soils[J].BMC Microbiology,2009,9:4.
    [22] SANTINI J M,SLY L I,SCHNAGL R D,et al.A new chemolithoautotrophic arsenite-oxidizing bacterium isolated from a goldmine:phylogenetic,physiological,and preliminary biochemical studies[J].Applied and Environmental Microbiology,2000,66:92-97.
  • 加载中
计量
  • 文章访问数:  1306
  • HTML全文浏览量:  17
  • PDF下载量:  107
  • 被引次数: 0
出版历程
  • 收稿日期:  2011-03-15
  • 修回日期:  2011-04-19
  • 刊出日期:  2011-10-25

目录

    /

    返回文章
    返回