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改进型重组基因酵母TR-GRIP1检测化合物甲状腺激素干扰活性

李 剑 任姝娟 马 梅 王子健

李 剑, 任姝娟, 马 梅, 王子健. 改进型重组基因酵母TR-GRIP1检测化合物甲状腺激素干扰活性[J]. 环境科学研究, 2011, 24(10): 1172-1177.
引用本文: 李 剑, 任姝娟, 马 梅, 王子健. 改进型重组基因酵母TR-GRIP1检测化合物甲状腺激素干扰活性[J]. 环境科学研究, 2011, 24(10): 1172-1177.
LI Jian, REN Shu-juan, MA Mei, WANG Zi-jian. Use of Modified Recombinant Gene Yeast TR-GRIP1 to Screen Chemicals for Thyroid Disrupting Activity[J]. Research of Environmental Sciences, 2011, 24(10): 1172-1177.
Citation: LI Jian, REN Shu-juan, MA Mei, WANG Zi-jian. Use of Modified Recombinant Gene Yeast TR-GRIP1 to Screen Chemicals for Thyroid Disrupting Activity[J]. Research of Environmental Sciences, 2011, 24(10): 1172-1177.

改进型重组基因酵母TR-GRIP1检测化合物甲状腺激素干扰活性

基金项目: 国家自然科学基金项目(41001351);高等学校博士学科点专项科研基金项目(20100003120024)

Use of Modified Recombinant Gene Yeast TR-GRIP1 to Screen Chemicals for Thyroid Disrupting Activity

  • 摘要: 应用酵母双杂交技术构建重组TR(甲状腺激素受体)基因酵母,用以检测类/抗甲状腺激素化合物及环境样品的甲状腺激素干扰活性. 提取并纯化含有TR基因的酵母表达质粒pGBT9-TR以及含有TR共激活因子GRIP1基因的酵母表达质粒pGAD424-GRIP1,将pGBT9-TR和pGAD424-GRIP1同时转化至酵母细胞Y187,以营养缺陷型培养基(SD/-Trp/-Leu)筛选阳性菌落,构建TR-GRIP1双杂交酵母. 考察该酵母与天然甲状腺激素——T3(三碘甲状腺原氨酸)的结合情况,确定最佳暴露时间,建立剂量-效应关系曲线. 结果表明:TR-GRIP1双杂交酵母能够与T3结合诱导β-半乳糖苷酶活性,选择暴露时间为2 h,T3诱导酶活性的EC50值为1.1×10-7 mol/L;构建的重组基因酵母TR-GRIP1提供了检测化合物甲状腺激素干扰活性的新方法.

     

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出版历程
  • 收稿日期:  2011-01-18
  • 修回日期:  2011-04-09
  • 刊出日期:  2011-10-25

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