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稀土元素钕对菲律宾蛤仔金属硫蛋白的诱导效应

付海防 孟范平 高 鹰 赵顺顺

付海防, 孟范平, 高 鹰, 赵顺顺. 稀土元素钕对菲律宾蛤仔金属硫蛋白的诱导效应[J]. 环境科学研究, 2011, 24(12): 1435-1440.
引用本文: 付海防, 孟范平, 高 鹰, 赵顺顺. 稀土元素钕对菲律宾蛤仔金属硫蛋白的诱导效应[J]. 环境科学研究, 2011, 24(12): 1435-1440.
FU Hai-fang, MENG Fan-ping, GAO Ying, ZHAO Shun-shun. Induction Effect of Metallothionein Synthesis in Ruditapes philippinarum Exposed to Rare Earth Element Nd[J]. Research of Environmental Sciences, 2011, 24(12): 1435-1440.
Citation: FU Hai-fang, MENG Fan-ping, GAO Ying, ZHAO Shun-shun. Induction Effect of Metallothionein Synthesis in Ruditapes philippinarum Exposed to Rare Earth Element Nd[J]. Research of Environmental Sciences, 2011, 24(12): 1435-1440.

稀土元素钕对菲律宾蛤仔金属硫蛋白的诱导效应

基金项目: 国家海洋公益性行业科研专项(201005012)

Induction Effect of Metallothionein Synthesis in Ruditapes philippinarum Exposed to Rare Earth Element Nd

  • 摘要: 稀土元素能否诱导海洋生物合成MTs(金属硫蛋白),将影响到MTs对海水重金属的指示作用. 以菲律宾蛤仔(Ruditapes philippinarum)为试验动物,采用含稀土Nd3+的人工海水对其进行暴露培养,测定蛤仔鳃部和内脏中MTs含量随暴露剂量和时间的变化. 结果表明,Nd3+暴露能够增加蛤仔体内MTs含量. ρ(Nd3+)为0.1~10 μg/L时能明显促进蛤仔鳃和内脏MTs的合成;蛤仔在ρ(Nd3+)为1 μg/L下暴露培养7 d,内脏MTs含量达到最高水平;同时,光谱扫描及SDS-PAGE分析表明,Nd3+诱导的蛤仔内脏中MTs在258 nm处有特征吸收峰,主要以二聚体和三聚体的形式存在,分子质量分别为13.0和18.1 ku.

     

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出版历程
  • 收稿日期:  2011-01-16
  • 修回日期:  2011-04-12
  • 刊出日期:  2011-12-25

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