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集胞藻胞外聚合物(EPS)与氯霉素的相互作用

付庆龙 张道勇 牟书勇 潘响亮

付庆龙, 张道勇, 牟书勇, 潘响亮. 集胞藻胞外聚合物(EPS)与氯霉素的相互作用[J]. 环境科学研究, 2012, 25(1): 58-62.
引用本文: 付庆龙, 张道勇, 牟书勇, 潘响亮. 集胞藻胞外聚合物(EPS)与氯霉素的相互作用[J]. 环境科学研究, 2012, 25(1): 58-62.
FU Qing-long, ZHANG Dao-yong, MU Shu-yong, PAN Xiang-liang. Interaction between Chloramphenicol and the Extracellular Polymeric Substances from Cyanobacterium Synechocystis sp.[J]. Research of Environmental Sciences, 2012, 25(1): 58-62.
Citation: FU Qing-long, ZHANG Dao-yong, MU Shu-yong, PAN Xiang-liang. Interaction between Chloramphenicol and the Extracellular Polymeric Substances from Cyanobacterium Synechocystis sp.[J]. Research of Environmental Sciences, 2012, 25(1): 58-62.

集胞藻胞外聚合物(EPS)与氯霉素的相互作用

基金项目: 中国科学院重要方向性项目(KZCX2-YW-335);中国科学院“百人计划”项目

Interaction between Chloramphenicol and the Extracellular Polymeric Substances from Cyanobacterium Synechocystis sp.

  • 摘要: 通过利用激发-发射矩阵(EEM)荧光光谱,研究蓝藻集胞藻EPS(胞外聚合物)与CAP(氯霉素)的相互作用.结果表明:EPS含有6个峰,其中峰A(Ex/Em=205/304)、峰B(Ex/Em=230/302)、峰C(Ex/Em=235/354)和峰D(Ex/Em=260/372)为类蛋白峰,峰E(Ex/Em=275/446)和峰F(Ex/Em=350/452)为类腐殖质峰.除峰F外,其他各峰都能被CAP猝灭,说明它们所代表的物质能够与CAP发生作用;而峰F则几乎不被CAP猝灭,即荧光峰F不与CAP发生作用.CAP与EPS中荧光基团的反应属于静态猝灭,生成稳定的不发荧光的EPS-CAP络合物,其有效猝灭常数为3.28~4.49,结合常数为4.54~8.13.EPS与CAP强的络合作用意味着环境中普遍存在的EPS可能深刻地影响CAP在水环境中的迁移与转化.

     

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  • 刊出日期:  2012-01-25

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