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Pb2+对城市污水处理厂污泥中苏云金芽孢杆菌发酵的影响

常明 摆亚军 孙启宏 陈晨 倪晋仁

常明, 摆亚军, 孙启宏, 陈晨, 倪晋仁. Pb2+对城市污水处理厂污泥中苏云金芽孢杆菌发酵的影响[J]. 环境科学研究, 2012, 25(8): 933-940.
引用本文: 常明, 摆亚军, 孙启宏, 陈晨, 倪晋仁. Pb2+对城市污水处理厂污泥中苏云金芽孢杆菌发酵的影响[J]. 环境科学研究, 2012, 25(8): 933-940.
CHANG Ming, BAI Ya-jun, SUN Qi-hong, CHEN Chen, NI Jin-ren. Effect of Lead Ions on Bacillus thuringiensisFermentation with Sewage Sludge as Medium[J]. Research of Environmental Sciences, 2012, 25(8): 933-940.
Citation: CHANG Ming, BAI Ya-jun, SUN Qi-hong, CHEN Chen, NI Jin-ren. Effect of Lead Ions on Bacillus thuringiensisFermentation with Sewage Sludge as Medium[J]. Research of Environmental Sciences, 2012, 25(8): 933-940.

Pb2+对城市污水处理厂污泥中苏云金芽孢杆菌发酵的影响

基金项目: 国家自然科学基金项目(41130743);国家水体污染控制与治理科技重大专项(2009ZX07208-002-001)

Effect of Lead Ions on Bacillus thuringiensisFermentation with Sewage Sludge as Medium

  • 摘要: 以城市污水处理厂污泥为培养基,研究了添加Pb2+对Bt(Bacillus thuringiensis,苏云金芽孢杆菌)生长和晶体蛋白合成的影响,同时分析了Bt发酵过程中有效态Pb含量(以ρ计)的变化,并采用FTIR(傅立叶红外光谱)和XPS(X射线光电子能谱),初步探讨了Pb2+在Bt表面的作用机制. 结果表明:污泥培养基在支持Bt生长代谢和降低重金属有效态含量方面均优于商用培养基,当初始ρ(Pb2+)高达400 mg/L时,与未添加Pb2+相比,污泥培养基中Bt活菌数与晶体蛋白产量约分别下降48%和54%,而商用培养基中约分别下降82%和75%,证实了污泥作为Bt发酵培养基的可行性;Bt对Pb2+有较好的耐受性,当ρ(有效态Pb)在80 mg/L以下时,Bt生长代谢不会受到显著影响. 光谱学分析表明,Bt对Pb2+有少量吸附,从而降低Bt发酵过程中Pb的生物有效性,吸附位点主要为羟基,部分CO和SO也参与了吸附反应.

     

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出版历程
  • 收稿日期:  2011-12-20
  • 修回日期:  2012-05-04
  • 刊出日期:  2012-08-25

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