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重金属离子胁迫对赤潮微藻的急性毒性

于小娣 师玥 周斌 王其翔 张鑫鑫 唐学玺 王悠

于小娣, 师玥, 周斌, 王其翔, 张鑫鑫, 唐学玺, 王悠. 重金属离子胁迫对赤潮微藻的急性毒性[J]. 环境科学研究, 2012, 25(9): 1047-1053.
引用本文: 于小娣, 师玥, 周斌, 王其翔, 张鑫鑫, 唐学玺, 王悠. 重金属离子胁迫对赤潮微藻的急性毒性[J]. 环境科学研究, 2012, 25(9): 1047-1053.
YU Xiao-di, SHI Yue, ZHOU Bin, WANG Qi-xiang, ZHANG Xin-xin, TANG Xue-xi, WANG You. Acute Toxic Effects of Heavy Metal Ions on Growth of Marine Bloom-Forming Microalgae[J]. Research of Environmental Sciences, 2012, 25(9): 1047-1053.
Citation: YU Xiao-di, SHI Yue, ZHOU Bin, WANG Qi-xiang, ZHANG Xin-xin, TANG Xue-xi, WANG You. Acute Toxic Effects of Heavy Metal Ions on Growth of Marine Bloom-Forming Microalgae[J]. Research of Environmental Sciences, 2012, 25(9): 1047-1053.

重金属离子胁迫对赤潮微藻的急性毒性

基金项目: 国家自然科学基金项目(41076066,31070458);海洋公益性行业科研专项(201105005)

Acute Toxic Effects of Heavy Metal Ions on Growth of Marine Bloom-Forming Microalgae

  • 摘要: 重金属污染和赤潮都是较为突出的海洋环境问题,为给相关研究提供基础试验数据,在实验室条件下研究了Cu2+、Cd2+、Zn2+对米氏凯伦藻和微小原甲藻的急性毒性效应. 通过2种藻的EC50(半数抑制浓度)比较了其敏感性差异,分析了不同浓度重金属胁迫对微藻的影响;同时,利用TEM (transmission electronic microscopy,透射电镜技术)和FCM (flow cytometry,流式细胞术)研究了重金属Cu2+的急性毒性胁迫在亚细胞水平上对米氏凯伦藻的损伤. 其中,Cu2+、Cd2+、Zn2+对微小原甲藻的48 h EC50和96 h EC50分别为0.514和0.276 mg/L、0.835和1.215 mg/L及4.376和7.976 mg/L;对米氏凯伦藻的48 h EC50和96 h EC50分别为1.881和1.881 mg/L、5.405和6.268 mg/L及13.134 和18.732 mg/L. 结果表明:2种微藻的种群相对增长率随着胁迫浓度的增加而下降,3种重金属离子的毒性表现出Cu2+>Cd2+>Zn2+的趋势,微小原甲藻对重金属胁迫更敏感;Cu2+胁迫可使米氏凯伦藻细胞体积增大,内容物复杂程度增高,但叶绿素a的MFI(mean fluorescence intensity,平均荧光强度)的变化不明显;Cu2+胁迫对藻膜结构的损伤较明显,但对线粒体及叶绿体的结构影响不明显.

     

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出版历程
  • 收稿日期:  2011-03-06
  • 修回日期:  2012-05-07
  • 刊出日期:  2012-09-25

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