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大气颗粒物对A549和HUVECs细胞的毒性作用

范兰兰 尚羽 张玲

范兰兰, 尚羽, 张玲. 大气颗粒物对A549和HUVECs细胞的毒性作用[J]. 环境科学研究, 2012, 25(10): 1166-1172.
引用本文: 范兰兰, 尚羽, 张玲. 大气颗粒物对A549和HUVECs细胞的毒性作用[J]. 环境科学研究, 2012, 25(10): 1166-1172.
FAN Lan-lan, SHANG Yu, ZHANG Ling. Toxic Effects of Ambient Particles on Human Lung Epithelial and Umbilical Vein Endothelial Cells[J]. Research of Environmental Sciences, 2012, 25(10): 1166-1172.
Citation: FAN Lan-lan, SHANG Yu, ZHANG Ling. Toxic Effects of Ambient Particles on Human Lung Epithelial and Umbilical Vein Endothelial Cells[J]. Research of Environmental Sciences, 2012, 25(10): 1166-1172.

大气颗粒物对A549和HUVECs细胞的毒性作用

基金项目: 国家水体污染控制与治理科技重大专项(2008ZX07526-003)国家自然科学基金青年科学基金项目(21107068);环境模拟与污染控制国家重点联合实验室(北京大学)开放课题(09K02ESPCP)

Toxic Effects of Ambient Particles on Human Lung Epithelial and Umbilical Vein Endothelial Cells

  • 摘要: 比较了A549和HUVECs 2种细胞对颗粒物的敏感度,以探讨大气颗粒物粒径对生物活性的影响. 采集北京市区PM10~2.5、PM2.5~0.1和PM0.1,将A549和HUVECs细胞暴露于不同浓度的颗粒物悬浮液24 h后,用噻唑蓝(MTT)法测定细胞存活率,并用LDH试剂盒测定细胞培养液中LDH(乳酸脱氢酶)的含量. 结果表明:随着染毒剂量的增大,细胞存活率逐渐降低,并且呈剂量-反应关系;培养液中LDH浓度呈剂量依赖型增加;当染毒剂量>200 μg/mL时,PM0.1的细胞致死率大于PM10~2.5和PM2.5~0.1(P<0.01);同一粒径的颗粒物对HUVECs的毒性比A549略大,但无统计意义. 因此,相对于粗颗粒物,细、超细颗粒物具有较大的细胞毒性,A549和HUVECs细胞对颗粒物的敏感度差异不显著.

     

  • [1] KROLL A, GUNSEL A, KLEMM O, et al.In vitro toxicology of ambient particulate matter:correlation of cellular effects with particle size and components.Environ Toxicol,2011,26(8):1-15.
    [2] FARINA F, SANCINI G, MANTECCA P, et al.The acute toxic effects of particulate matter in mouse lung are related to size and season of collection.Toxicology Letters,2011,202(3):209-217.
    [3] LOPEZ-VILLARRUBIA E, INIGUEZ C, PERAL N, et al.Characterizing mortality effects of particulate matter size fractions in the two capital cities of the Canary Islands.Environ Res,2012,112:129-138.
    [4] POPE C A, BURNETT R T, THUN M J, et al.Lung cancer, cardiopulmonary mortality, and long-time exposure to fine particulate air pollution.JAMA,2002,287(9):1132-1141.
    [5] TERZANO C, DI STEFANO F, CONTI V, et al.Air pollution ultrafine particles:toxicity beyond the lung.European Review for Medical and Pharmacological Sciences,2010,14(10):809-821.
    [6] 刘芳盈,丁明玉,李杰,等.燃煤PM2.5不同组分对血管内皮细胞的毒性.环境科学研究,2011,24(6):684-690.
    [7] DELFINO R J, SIOUTAS C, MALIK S.Potential role of ultrafine particles in associations between airborne particle mass and cardiovascular health.Environmental Health Perspectives,2005,113(8):934-946.
    [8] HOEK G, BOOGAARD H, KNOL A, et al.Concentration response functions for ultrafine particles and all-cause mortality and hospital admissions:results of a European expert panel elicitation.Environ Sci Technol,2010,44(1):476-482.
    [9] POLITIS M, PILINIS C, LEKKAS T D.Ultrafine particles(UFP) and health effects.Dangerous.Like no other PM? Review and analysis.Global Nest Journal,2008,10(3):439-452.
    [10] 陈威,郭新彪.大气颗粒物对心血管系统影响的研究进展.环境与健康杂志,2005,22(6):89-91.
    [11] 杨辉,杨丹凤,张华山,等.4种典型纳米材料对小鼠胚胎成纤维细胞毒性的初步研究.生态毒理学报,2007,2(4):427-434.
    [12] 曲凡,丁文军,伊硕,等.PM2.5对肺泡Ⅱ型上皮细胞MLE-12的毒性研究.毒理学杂志,2010,24(1):19-23.
    [13] MOLLER P, JACOBSEN N R, FOLKMANN J K, et al.Role of oxidative damage in toxicity of particulates.Free Radical Research,2010,44(1):1-46.
    [14] TAO F B, GONZALEZ-FLECHA L, KOBZIK L.Reactive oxygen species in pulmonary inflammation by ambient particulates.Free Radical Biology and Medicine,2003,35(4):327-340.
    [15] KONCZOL M, EBELING S, GOLDENBERG E, et al.Cytotoxicity and genotoxicity of size-fractionated iron oxide(magnetite) in A549 human lung epithelial cells:role of ROS, JNK, and NF-kappa B.Chemical Research in Toxicology,2011,24(9):1460-1475.
    [16] KOUASSI K S, BILLET S,GARCON G, et al.Oxidative damage induced in A549 cells by physically and chemically characterized air particulate matter (PM2.5) collected in Abidjan, Cote d′Ivoire.Journal of Applied Toxicology,2010,30(4):310-320.
    [17] MONTIEL-DAVALOS A, BARRA-SANCHEZ M D, VENTURA-GALLEGOS J L, et al.Oxidative stress and apoptosis are induced in human endothelial cells exposed to urban particulate matter.Toxicology in Vitro,2010,24(1):135-141.
    [18] L Senlin, FENG Man, YAO Zhenkun, et al.Physicochemical characterization and cytotoxicity of ambient coarse, fine, and ultrafine particulate matters in Shanghai atmosphere.Atmos Environ,2011,45(3):736-744.
    [19] HAPPO M S, SALONEN R O, HALINEN A I, et al.Inflammation and tissue damage in mouse lung by single and repeated dosing of urban air coarse and fine particles collected from six European cities.Inhalation Toxicology,2010,22(5):402-416.
    [20] WESSELS A, BIRMILI W, ALBRECHT C, et al.Oxidant generation and toxicity of size-fractionated ambient particles in human lung epithelial cells.Environ Sci Technol,2010,44(9):3539-3545.
    [21] GUALTIERI M, OVREVIK J, HOLME J A, et al.Differences in cytotoxicity versus pro-inflammatory potency of different PM fractions in human epithelial lung cells.Toxicology in Vitro,2010,24(1):29-39.
    [22] OSORNIO-VARGAS A R, BONNER J C, ALFARO-MORENO E, et al.Pro-inflammatory and cytotoxic effects of Mexico City air pollution particulate matter in vitro are dependent on particle size and composition.Environmental Health Perspectives,2003,111(10):1289-1293.
    [23] SCHWARZE P E, OVREVIK J, HETLAND R B, et al.Importance of size and composition of particles for effects on cells in vitro.Inhalation Toxicology,2007,19:17-22.
    [24] STEENHOF M, GOSENS I, STRAK M, et al.In vitro toxicity of particulate matter (PM) collected at different sites in the Netherlands is associated with PM composition, size fraction and oxidative potential-the RAPTES project.Particle and Fibre Toxicology,2011,26(8):1-15.
    [25] 李怡,朱彤.大气颗粒物致机体损伤的OH自由基机制.生态毒理学报,2007,2(2):142-149.
    [26] HAN J Y, TAKESHITA K, UTSUMI H.Noninvasive detection of hydroxyl radical generation in lung by diesel exhaust particles.Free Radical Biology and Medicine,2001,30(5):516-525.
    [27] CHUNG S W, CHUNG H Y, TORIBA A, et al.An environmental quinoid polycyclic aromatic hydrocarbon, acenaphthenequinone, modulates cyclooxygenase-2 expression through reactive oxygen species generation and nuclear factor kappa B activation in A549 cells.Toxicological Sciences,2007,95(2):348-355.
    [28] 孙秀明,郭新彪,邓芙蓉.内毒素对可吸入颗粒物所诱发的巨噬细胞毒性和前炎性细胞因子mRNA表达的影响.环境与健康杂志,2011,28(7):565-568.
    [29] CAMATINI M, CORVAJA V, PEZZOLATO E, et al.PM10-biogenic fraction drives the seasonal variation of proinflammatory response in A549 cells.Environ Toxicol,2012,27(2):63-73.
    [30] 洪也,马雁军,李潮流,等.沈阳冬季灰霾日大气颗粒物元素粒径分布特征.环境科学研究,2011,24(6):637-644.
    [31] 原福胜,马亚萍,武忠诚.不同粒径大气颗粒物中金属元素含量及其对人双核淋巴细胞微核率的影响.卫生研究,1999,28(1):23-24.
    [32] 周家斌,王铁冠,黄碧云,等.不同粒径大气颗粒物中多环芳烃的含量及分布特征.环境科学,2005,26(2):40-44.
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出版历程
  • 收稿日期:  2012-02-14
  • 修回日期:  2012-06-04
  • 刊出日期:  2012-10-25

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