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海洋石油降解酵母的分离鉴定与降解特性

刘杰凤 马 超 刘正辉 王 春 周 天

刘杰凤, 马 超, 刘正辉, 王 春, 周 天. 海洋石油降解酵母的分离鉴定与降解特性[J]. 环境科学研究, 2013, 26(8): 899-905.
引用本文: 刘杰凤, 马 超, 刘正辉, 王 春, 周 天. 海洋石油降解酵母的分离鉴定与降解特性[J]. 环境科学研究, 2013, 26(8): 899-905.
LIU Jie-feng, MA Chao, LIU Zheng-hui, WANG Chun, ZHOU Tian. Isolation and Identification of Marine Petroleum-Degrading Yeast and Their Biodegradation Characteristics[J]. Research of Environmental Sciences, 2013, 26(8): 899-905.
Citation: LIU Jie-feng, MA Chao, LIU Zheng-hui, WANG Chun, ZHOU Tian. Isolation and Identification of Marine Petroleum-Degrading Yeast and Their Biodegradation Characteristics[J]. Research of Environmental Sciences, 2013, 26(8): 899-905.

海洋石油降解酵母的分离鉴定与降解特性

基金项目: 广东省海洋渔业科技推广专项(A201101B03,A201201C03)

Isolation and Identification of Marine Petroleum-Degrading Yeast and Their Biodegradation Characteristics

  • 摘要: 以原油为唯一碳源,采用富集培养、平板涂布分离、平板划线纯化及摇瓶复筛等方法,从表层海水或海泥中分离得到2株具有较强石油降解能力的菌株SYB-5和SYB-2. 根据菌落及菌体形态、生理生化特征和分子生物学分析、鉴定显示,SYB-5为季也蒙毕赤酵母(Meyerozyma guilliermondii/Pichia guilliermondii),SYB-2为长孢洛德酵母(Lodderomyces elongisporus). 对2株酵母菌的石油降解性能的研究结果表明,SYB-5和SYB-2以原油组分作为碳源,以(NH4)2SO4和(NH4)3PO4作为氮源,在ρ(NaCl)为30g/L、氧气充足的条件下,生长的最适温度分别为36和32℃,pH均为7.0. 在最适生长条件下,培养5d后的原油降解率分别达到45.8%和34.4%. 当2株菌混合培养时,培养5d的原油降解率可提高到53.9%,培养8d时达到56.4%,说明2株菌利用原油作碳源生长时具有协同作用.

     

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出版历程
  • 收稿日期:  2012-12-23
  • 修回日期:  2013-04-23
  • 刊出日期:  2013-08-25

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