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不同pH条件下CdCl2对紫花苜蓿种子萌发的胁迫效应

王文斌 金 樑 李 晶 邓志刚 杨 阳 王晓娟

王文斌, 金 樑, 李 晶, 邓志刚, 杨 阳, 王晓娟. 不同pH条件下CdCl2对紫花苜蓿种子萌发的胁迫效应[J]. 环境科学研究, 2013, 26(10): 1095-1102.
引用本文: 王文斌, 金 樑, 李 晶, 邓志刚, 杨 阳, 王晓娟. 不同pH条件下CdCl2对紫花苜蓿种子萌发的胁迫效应[J]. 环境科学研究, 2013, 26(10): 1095-1102.
WANG Wen-bin, JIN Liang, LI Jing, DENG Zhi-gang, YANG Yang, WANG Xiao-juan. Effects of CdCl2Stress under Different pH to Seed Germination in Alfalfa[J]. Research of Environmental Sciences, 2013, 26(10): 1095-1102.
Citation: WANG Wen-bin, JIN Liang, LI Jing, DENG Zhi-gang, YANG Yang, WANG Xiao-juan. Effects of CdCl2Stress under Different pH to Seed Germination in Alfalfa[J]. Research of Environmental Sciences, 2013, 26(10): 1095-1102.

不同pH条件下CdCl2对紫花苜蓿种子萌发的胁迫效应

基金项目: 国家自然科学基金项目(31270558)

Effects of CdCl2Stress under Different pH to Seed Germination in Alfalfa

  • 摘要: 采用培养皿滤纸萌发试验,研究在3种pH(4.0、6.0和8.0)条件下,不同质量浓度(0、5、10、20、40和80mg/L)的CdCl2溶液对紫花苜蓿种子萌发的胁迫效应. 结果表明,低质量浓度的CdCl2(≤20mg/L)显著提高了紫花苜蓿种子的发芽率、发芽势、发芽指数和活力指数,ρ(CdCl2)为20mg/L、pH为6.0时活力指数与对照组相比明显增加,达到177.52±16.61;随着ρ(CdCl2)的提高,CdCl2对苜蓿幼苗生长的促进效应不断增强,ρ(CdCl2)为20mg/L时,3种pH条件下的芽鲜质量分别为(6.60±0.11)、(6.68±0.20)和(9.51±0.16)mg,根鲜质量分别为(6.71±0.10)、(7.09±0.08)和(9.10±0.08)mg,达到最大; 当ρ(CdCl2)达到40和80mg/L时,紫花苜蓿种子萌发和幼苗生长则受到显著抑制,ρ(CdCl2)为80mg/L时发芽率和活力指数分别仅为对照组的14.8%和46.5%;ρ(CdCl2)为80mg/L时芽鲜质量最低,3种pH条件下分别为(3.48±0.15)、(4.03±0.19)和(7.00±0.22)mg. 研究还发现,紫花苜蓿种子发芽率和活力指数随pH降低而降低,弱碱(pH=8.0)条件下紫花苜蓿在ρ(CdCl2)为80mg/L时的发芽率为66.40%±3.19%,大于酸性条件(pH=4.0、6.0)下的发芽率(11.20%±3.61%、9.20%±4.22%),而活力指数(93.90±10.71)也高于酸性条件(pH=4.0、6.0)下的活力指数(49.77±3.25、56.67±3.48),表明pH由酸性到碱性的变化过程能够缓解CdCl2对紫花苜蓿种子萌发和幼苗生长的毒性.

     

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出版历程
  • 收稿日期:  2013-03-26
  • 修回日期:  2013-08-16
  • 刊出日期:  2013-10-25

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