留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

文蛤谷胱甘肽抗氧化系统对北部湾沉积物中可提取态重金属的响应

付文超 孟范平 王 怡 王志峰 王 群

付文超, 孟范平, 王 怡, 王志峰, 王 群. 文蛤谷胱甘肽抗氧化系统对北部湾沉积物中可提取态重金属的响应[J]. 环境科学研究, 2014, 27(2): 186-195.
引用本文: 付文超, 孟范平, 王 怡, 王志峰, 王 群. 文蛤谷胱甘肽抗氧化系统对北部湾沉积物中可提取态重金属的响应[J]. 环境科学研究, 2014, 27(2): 186-195.
FU Wen-chao, MENG Fan-ping, WANG Yi, WANG Zhi-feng, WANG Qun. Response of Glutathione Antioxidant System in Bivalve Meretrix meretrix to Speciation and Content of Heavy Metals in Coastal Sediments in Beibu Bay, China[J]. Research of Environmental Sciences, 2014, 27(2): 186-195.
Citation: FU Wen-chao, MENG Fan-ping, WANG Yi, WANG Zhi-feng, WANG Qun. Response of Glutathione Antioxidant System in Bivalve Meretrix meretrix to Speciation and Content of Heavy Metals in Coastal Sediments in Beibu Bay, China[J]. Research of Environmental Sciences, 2014, 27(2): 186-195.

文蛤谷胱甘肽抗氧化系统对北部湾沉积物中可提取态重金属的响应

基金项目: 国家海洋公益性行业科研专项(201005012);国家自然科学基金项目(41240040)

Response of Glutathione Antioxidant System in Bivalve Meretrix meretrix to Speciation and Content of Heavy Metals in Coastal Sediments in Beibu Bay, China

  • 摘要: 为识别海洋双壳类谷胱甘肽抗氧化系统对滨海沉积物重金属可提取态和总量的响应特征差异,分析了北部湾潮间带沉积物中不同形态Cu、Pb、Zn、Cd、Cr的质量分数,文蛤(Meretrix meretrix)鳃组织中w(GSH)(GSH为还原型谷胱甘肽)、w(GSSG)(GSSG为氧化型谷胱甘肽)、GPx(谷胱甘肽过氧化物酶)和GST(谷胱甘肽硫转移酶)活性,并计算w(GSH)/w(GSSG)、tGSH(总谷胱甘肽)含量和OSI(氧化逆境指数). 结果表明:w(TE-Pb)(TE-Pb为可提取态Pb,下同)、w(TE-Cr)、w(TE-Cu)、w(TE-Zn)和w(TE-Cd)平均值分别为13.40、1.93、13.19、30.09和0.10mg/kg; w(TE-Cd)占w(T-Cd)(T-Cd为总Cd,下同)的60.2%~98.1%,并且主要为弱酸溶解态和有机物及硫化物结合态;部分采样点w(TE-Pb)和w(TE-Zn)较高,二者占w(T-Pb)和w(T-Zn)的平均值分别为44.6%和56.6%;Cr、Cu分别在全部或大多数采样点以残渣态为主,可提取态含量平均仅占总量的17.9%和36.5%. 重金属的可提取态质量分数与总量的空间分布基本一致(Cr除外). 文蛤鳃中w(GSH)、w(GSSG)分别为4.07~6.06、4.01~6.59mg/g. 抗氧化指标对沉积物中重金属总量和可提取态质量分数均有显著响应的为w(GSSG)与Cd,w(GSH)/w(GSSG)与Cr和Cd,OSI与Cr和Cd. 然而,w(GSH)/w(GSSG)和OSI只对w(T-Zn)有良好响应;GST只与w(TE-Cu)、w(TE-Zn)显著相关. 某些抗氧化指标对重金属总量和可提取态质量分数表现出不同程度的响应,因此,不能单独依据双壳类对重金属总量的生化响应特征来筛选用于沉积物综合毒性评价的生物标志物.

     

  • [1] SELVARAJ K,RAM M V,SZEFER P.Evaluation of metal contamination in coastal sediments of the Bay of Bengal,India:geochemical and statistical approaches.Marine Pollution Bulletin,2004,9(3):174-185.
    [2] RUMISHA C,ELSKENS M,LEERMAKERS M,et al.Trace metal pollution and its influence on the community structure of soft bottom molluscs in intertidal areas of the Dar es Salaam coast,Tanzania.Marine Pollution Bulletin,2012,4(3):521-531.
    [3] 孟范平,高鹰,赵顺顺,等.双壳类分子生物标志物对海水重金属的响应评述.中国海洋大学学报,2011,1(5):100-109.
    [4] VAN DER OOST R,BEYER J,VERMEULEN N P E.Fish bioaccumulation and biomarkers in environmental risk assessment:a review.Environ Toxicol Pharmacol,2003,3(2):57-149.
    [5] REGOLI F,PRINCIPATO G.Glutathione,glutathione-dependent and antioxidant enzymes in mussel,Mytilus galloprovincialis,exposed to metals under field and laboratory conditions:implications for the use of biochemical biomarkers.Aquatic Toxicology,1995,1(2):143-164.
    [6] OLSVIK P A,KRISTENSEN T,WAAGBO R,et al.mRNA expression of antioxidant enzymes (SOD,CAT and GSH-Px) and lipid peroxidative stress in liver of Atlantic salmon (Salmo salar) exposed to hyperoxic water during smoltification.Comparative Biochemistry and Physiology:Part C,2005,1(3):314-323.
    [7] CARNEY A B,ALBERTSSON E,STURVE J,et al.Oxidative stress,evident in antioxidant defences and damage products,in rainbow trout caged outside a sewage treatment plant.Ecotoxicology and Environmental Safety,2008,0(3):370-378.
    [8] S'WIERGOSZ-KOWALEWSKA R,BEDNARSKA A,KAFEL A.Glutathione levels and enzyme activity in the tissues of bank vole Clethrionomys glareolus chronically exposed to a mixture of metal contaminants.Chemosphere,2006,5(6):963-974.
    [9] FREITAS R,COSTA E,VELEZ C,et al.Looking for suitable biomarkers in benthic macroinvertebrates inhabiting coastal areas with low metal contamination:comparison between the bivalve Cerastoderma edule and the polychaete Diopatra neapolitana.Ecotoxicology and Environmental Safety,2012,5:109-118.
    [10] OLIVA M,JOS V J,GRAVATO C,et al.Oxidative stress biomarkers in Senegal sole,Solea senegalensis,to assess the impact of heavy metal pollution in a Huelva estuary (SW Spain):seasonal and spatial variation.Ecotoxicology and Environmental Safety,2012,5:151-162.
    [11] WON E J,HONG S,RA K,et al.Evaluation of the potential impact of polluted sediments using Manila clam Ruditapes philippinarum:bioaccumulation and biomarker responses.Environmental Science and Pollution Research,2012,9(7):2570-2580.
    [12] HEIER L S,LIEN I B,STRMSENG A E.Speciation of lead,copper,zinc and antimony in water draining a shooting range:time dependant metal accumulation and biomarker responses in brown trout (Salmo trutta L.).Sci Total Environ,2009,7(13):4047-4055.
    [13] FAN Wenhong,WANG Wenxiong.Sediment geochemical controls on Cd,Cr,and Zn assimilation by the clam ruditapes philippinarum.Environmental Toxicology and Chemistry,2001,0(10):2309-2317.
    [14] ABD EL-AZIM H,EL-MOSELHYKH M.Determination and partitioning of metals in sediments along the Suez Canal by sequential extraction.Journal of Marine Systems,2003,6(2):363-374.
    [15] 姜霞,石志芳,刘锋,等.疏浚对梅粱湾表层沉积物重金属赋存形态及其生物毒性的影响.环境科学研究,2010,3(9):1151-1157.
    [16] 谭伟福,蒋波,廖铮.广西北部湾经济区发展规划实施对滨海生态环境的影响分析.广西科学院学报,2009,5(1):50-53,7.
    [17] 王艳,方展强,周海云,等.北部湾海域江豚体内重金属含量及分布.海洋环境科学,2008,7(1):63-66.
    [18] 张志锋,王燕,韩庚辰,等.北部湾沉积物中重金属元素的地球化学特征及物源初探.海洋学报,2013,5(2):72-81.
    [19] QUEVAUVILLER P,RAURET G,LPEZ-SNCHEZ J F,et al.Certification of trace metal extractable contents in a sediment reference material (CRM 601) following a three-step sequential extraction procedure.Sci Total Environ,1997,5(2):223-234.
    [20] 国家质量监督检验检疫总局,国家标准化管理委员会.GB 17378—2007海洋监测规范.北京:中国标准出版社,2008.
    [21] 付文超,孟范平,王志峰,等.北部湾潮间带沉积物和双壳类动物中的重金属:污染特征与生物积累.环境科学学报,2013,3(5):1401-1409.
    [22] RAURET G,LOPEZ-SANCHEZ J F,SAHUQUILLO A,et al.Improvement of the BCR three step sequential extraction procedure prior to the certification of new sediment and soil reference materials.Journal of Environmental Monitoring,1999,1(3):57-61.
    [23] NEMATI K,BAKAR N K U,ABAS M R,et al.Speciation of heavy metals by modified BCR sequential extraction procedure in different depths of sediments from Sungai Buloh,Selangor,Malaysia.J Hazard Mater,2011,2(1):402-410.
    [24] HABIG W H,JAKOBY W B.Assays for differentiation of glutathione S-transferase.Methods in Enzymology,1981,7(4):398-405.
    [25] 汤建林,周世文,徐传福.分光光度法测定小鼠组织中谷胱甘肽过氧化物酶活力.第三军医大学学报,1996,6(9):551-552.
    [26] 张迺哲,宋玉忱.组织中氧化型和还原型谷胱甘肽荧光测定法.生物化学与生物物理进展,1993(2):136-138.
    [27] ZHOU Yanwu,ZHAO Bo,PENG Yisheng,et al.Influence of mangrove reforestation on heavy metal accumulation and speciation in intertidal sediments.Marine Pollution Bulletin,2010,0(8):1319-1324.
    [28] CHAKRABORTY P,RAGHUNADH BABU P V,SARMA V V.A study of lead and cadmium speciation in some estuarine and coastal sediments.Chemical Geology,2012,4/295:217-225.
    [29] TESSIER A,CAMPBELL P G C.Partitioning of trace metals in sediments:relationships with bioavailability.Hydrobiologia,1987,9(1):43-52.
    [30] HALLIWELL B,GUTTERIDGE J M C.Free radicals in biology and medicine.Oxford:Oxford University Press,1999.
    [31] ALMEIDA E A,MIYAMOTO S,BAINY A C D,et al.Protective effect of phospholipid hydroperoxide glutathione peroxidase (PHGPx) against lipid peroxidation in mussels Perna perna exposed to different metals.Marine Pollution Bulletin,2004,9:386-392.
    [32] COSSU C,DOYOTTE A,BABUT M,et al.Antioxidant biomarkers in freshwater bivalves,Unio tumidus,in response to different contamination profiles of aquatic sediments.Ecotoxicology and Environmental Safety,2000,5(2):106-121.
    [33] TAYLOR A M,MAHER W A.Exposure-dose-response of Anadara trapezia to metal contaminated estuarine sediments:1.cadmium spiked sediments.Aquatic Toxicology,2012,9:234-242.
    [34] JILLETTE N,CAMMACK L,LOWENSTEIN M,et al.Down-regulation of activity and expression of three transport-related proteins in the gills of the euryhaline green crab,Carcinus maenas,in response to high salinity acclimation.Comparative Biochemistry and Physiology:Part A.Molecular & Integrative Physiology,2011,8(2):189-193.
    [35] HAMZA-CHAFFAI A,AMIARD J C,PELLERIN J,et al.The potential use of metallothionein in the clam Ruditapes decussatus as a biomarker of in situ metal exposure.Comparative Biochemistry and Physiology:Part C,2000,7(2):185-197.
    [36] 赵顺顺.海水重金属单一及复合污染对双壳类金属硫蛋白的影响和非生物因子的干扰.青岛:中国海洋大学,2012.
    [37] ROMO M,GNASSIA-BARELLI M.Metal distribution in different tissues and in subcellular fractions of the Mediterranean clam Ruditapes decussatus treated with cadmium,copper and zinc.Comparative Biochemistry and Physiology:Part C,1995,1(3):457-463.
    [38] MARIGMEZ I,SOTO M,CAJARAVILLE M P,et al.Cellular and subcellular distribution of metals in mollusks.Microscopy Research and Technique,2002,6(5):358-392.
    [39] 于瑞莲,胡恭任.泉州湾沉积物重金属形态特征及生态风险.华侨大学学报,2008,9(3):419-423.
    [40] 鲁双凤,王鹏,王军广,等.潮间带沉积物中重金属污染评价及生物有效性研究进展.热带林业,2011,9(1):17-20.
    [41] WANG Wenxiong,FISHER N S.Assimilation efficiencies of chemical contaminants in aquatic invertebrates:a synthesis.Environmental Toxicology and Chemistry,1999,8(9):2034-2045.
    [42] FERNNDEZ B,CAMPILLO J A,MARTNEZ-GMEZ C,et al.Antioxidant responses in gills of mussel (Mytilus galloprovincialis) as biomarkers of environmental stress along the Spanish Mediterranean coast.Aquatic Toxicology,2010,9(2):186-197.
    [43] WANG Zaosheng,YAN Changzhou,VULPE C D,et al.Incorporation of in situ exposure and biomarkers response in clams Ruditapes philippinarum for assessment of metal pollution in coastal areas from the Maluan Bay of China.Marine Pollution Bulletin,2012,4(1):90-98.
  • 加载中
计量
  • 文章访问数:  1364
  • HTML全文浏览量:  5
  • PDF下载量:  186
  • 被引次数: 0
出版历程
  • 收稿日期:  2013-08-09
  • 修回日期:  2013-10-01
  • 刊出日期:  2014-02-25

目录

    /

    返回文章
    返回