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超声灭活水中隐孢子虫影响因素及机制

李绍峰 冉治霖 胡健龙 张朝升

李绍峰, 冉治霖, 胡健龙, 张朝升. 超声灭活水中隐孢子虫影响因素及机制[J]. 环境科学研究, 2014, 27(4): 434-440.
引用本文: 李绍峰, 冉治霖, 胡健龙, 张朝升. 超声灭活水中隐孢子虫影响因素及机制[J]. 环境科学研究, 2014, 27(4): 434-440.
LI Shao-feng, RAN Zhi-lin, HU Jian-long, ZHANG Chao-sheng. Influence Factors and Mechanism of Ultrasonic Inactivating Cryptosporidium in Water[J]. Research of Environmental Sciences, 2014, 27(4): 434-440.
Citation: LI Shao-feng, RAN Zhi-lin, HU Jian-long, ZHANG Chao-sheng. Influence Factors and Mechanism of Ultrasonic Inactivating Cryptosporidium in Water[J]. Research of Environmental Sciences, 2014, 27(4): 434-440.

超声灭活水中隐孢子虫影响因素及机制

基金项目: 广东省科技计划项目(2011B030800018);深圳市科技计划项目(JCYJ20120617141700417);广东省自然科学基金项目(S2012040007855)

Influence Factors and Mechanism of Ultrasonic Inactivating Cryptosporidium in Water

  • 摘要: 采用超声灭活隐孢子虫卵囊,超声频率为19.8kHz,功率为151W,温度控制在(20±1)℃, 探讨了浊度、ρ(腐殖酸)及无机离子质量浓度对超声灭活隐孢子虫的影响,并通过扫描电镜观察及动力学分析探究了超声灭活隐孢子虫的机制. 结果表明:在浊度为0~21.46NTU范围内,隐孢子虫的灭活率先升后降,浊度为1.13NTU时灭活率最高,为94.7%;隐孢子虫灭活率随ρ(腐殖酸)的增加而降低,当ρ(腐殖酸)为50.0mg/L时灭活率降至78.4%. Cu2+、Mn2+和SO42-对灭活率影响较小,当ρ(Cu2+)为0.5mg/L时,隐孢子虫灭活率(93.2%)比未添加Cu2+时增加了0.7%;ρ(CO32-)增加,隐孢子虫灭活率下降,当ρ(CO32-)为150mg/L时灭活率降至82.5%. 扫描电镜形态学观察表明,超声可破坏隐孢子虫细胞结构;动力学分析表明,自由基氧化作用对灭活率的贡献为15.6%,说明超声灭活隐孢子虫的主要机制为空化作用产生的机械剪切作用.

     

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出版历程
  • 收稿日期:  2013-08-06
  • 修回日期:  2013-12-05
  • 刊出日期:  2014-04-25

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