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稀酸预处理铜藻制备生物乙醇工艺

蒋媛媛 包海军 曾淦宁 艾 宁 王铁杆 张茹霞 吴 勇

蒋媛媛, 包海军, 曾淦宁, 艾 宁, 王铁杆, 张茹霞, 吴 勇. 稀酸预处理铜藻制备生物乙醇工艺[J]. 环境科学研究, 2014, 27(7): 804-812.
引用本文: 蒋媛媛, 包海军, 曾淦宁, 艾 宁, 王铁杆, 张茹霞, 吴 勇. 稀酸预处理铜藻制备生物乙醇工艺[J]. 环境科学研究, 2014, 27(7): 804-812.
JIANG Yuan-yuan, BAO Hai-jun, ZENG Gan-ning, AI Ning, WANG Tie-gan, ZHANG Ru-xia, WU Yong. Optimization of Sargassum horneri Pretreatment Process Using Diluted Acid for Bioethanol[J]. Research of Environmental Sciences, 2014, 27(7): 804-812.
Citation: JIANG Yuan-yuan, BAO Hai-jun, ZENG Gan-ning, AI Ning, WANG Tie-gan, ZHANG Ru-xia, WU Yong. Optimization of Sargassum horneri Pretreatment Process Using Diluted Acid for Bioethanol[J]. Research of Environmental Sciences, 2014, 27(7): 804-812.

稀酸预处理铜藻制备生物乙醇工艺

基金项目: 浙江省自然科学基金项目(LY12D06004);浙江省科技厅公益项目(2013C33005)

Optimization of Sargassum horneri Pretreatment Process Using Diluted Acid for Bioethanol

  • 摘要: 选取暖温带海洋生态环境生物修复的首选物种——铜藻(Sargassum horneri)为原料进行生物乙醇的制备,以稀硫酸水解后的还原糖收率为响应值,考察水解温度、液固比、水解时间和w(H2SO4)等参数对水解效率的影响. 为优化稀酸水解铜藻预处理的工艺条件,在单因素试验的基础上,利用Box-Benhnken中心组合设计法和响应面分析法,建立稀酸预处理工艺参数的回归模型,并与酶水解及发酵相结合验证了铜藻稀酸预处理效果. 结果表明:①稀酸水解铜藻的最优工艺参数. 水解温度为120 ℃,液固比为20∶1,水解时间为2.00 h,w(H2SO4)为4.50%. ②稀酸水解铜藻过程中各影响因素之间存在交互作用,水解时间和w(H2SO4)的非线性作用显著. ③对经最佳稀酸预处理工艺处理后的铜藻粉进行酶水解,其还原糖收率为44.05%,是未预处理下的8.14倍,并且后续进行发酵后,乙醇产率达7.80%,是未预处理下的2.00倍. 表明铜藻是一种潜在的生物乙醇原料,稀酸预处理方法对铜藻生物乙醇的制备行之有效.

     

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出版历程
  • 收稿日期:  2013-10-15
  • 修回日期:  2014-03-12
  • 刊出日期:  2014-07-25

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