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松花江吉林段土壤有机组分的雄激素干扰效应

李 剑 李沫蕊 孔东东 韩绍伦

李 剑, 李沫蕊, 孔东东, 韩绍伦. 松花江吉林段土壤有机组分的雄激素干扰效应[J]. 环境科学研究, 2014, 27(8): 898-903.
引用本文: 李 剑, 李沫蕊, 孔东东, 韩绍伦. 松花江吉林段土壤有机组分的雄激素干扰效应[J]. 环境科学研究, 2014, 27(8): 898-903.
LI Jian, LI Mo-rui, KONG Dong-dong, HAN Shao-lun. The Androgenic/Anti-Androgenic Effects of Soil Organic Extracts from Songhua River Basin in Jilin[J]. Research of Environmental Sciences, 2014, 27(8): 898-903.
Citation: LI Jian, LI Mo-rui, KONG Dong-dong, HAN Shao-lun. The Androgenic/Anti-Androgenic Effects of Soil Organic Extracts from Songhua River Basin in Jilin[J]. Research of Environmental Sciences, 2014, 27(8): 898-903.

松花江吉林段土壤有机组分的雄激素干扰效应

基金项目: 国家自然科学青年基金项目(41001351);高等学校博士学科点专项科研基金资助课题(20100003120024)

The Androgenic/Anti-Androgenic Effects of Soil Organic Extracts from Songhua River Basin in Jilin

  • 摘要: 利用重组AR(雄激素受体)基因酵母,结合土壤样品索氏提取、富集和净化方法,开展松花江流域吉林段土壤有机组分雄激素干扰效应及其分布特征研究. 结果表明:所有样品均未检测出显著的AR诱导活性;部分样品检测出AR抑制活性,检出率为62.50%,最大抑制率为61.74%;采用RIC20(抑制率为20.00%时,96孔板每孔所需要的土壤当量,单位为mg/well)表征样品的抑制活性,RIC20在1.01~9.03 mg/well之间, 表明松花江吉林段土壤中存在AR抑制活性物质,可能具有潜在的环境危害. 研究区土壤雄激素干扰效应的分布特征表明,干扰效应分布与产业布局呈一定的相关性;工业区的AR抑制活性明显高于农田区,表明化工原料及产品的生产和使用及工业废水的排放可能是该地区土壤中AR抑制活性的重要来源.

     

  • [1] 韩伟,李艳霞,杨明,等.环境雄激素的危害﹑来源与环境行为.生态学报,2010,0(6):1594-1603.HAN Wei,LI Xiaoxia,YANG Ming,et al.Effects,sources and behaviors of environmental androgens.Acta Ecologica Sinica,2010,0(6):1594-1603.
    [2] 胡晓齐,王晶晶,王厚鹏,等.稀有的鲫雄激素受体基因的克隆和内分泌干扰物对其表达的影响.西北农业学报,2011,0(2):8-14.HU Xiaoqi,WANG Jingjing,WANG Houpeng,et al.Full-length cDNA cloning of androgen receptor gene from rare minnow(Gobiocypris rarus)and effect of endocrine disrupting chemicals on its mRNA expression.Acta Agriculturae Boreali-Occidentalis Sinica,2011,0(2):8-14.
    [3] 李靖华,王晓春,杨季红.雄激素受体与乳腺癌关系的研究进展.河北医药,2011,3(2):259-260.
    [4] 李雅娜,王东,殷彦君.外源性雄激素对大鼠胸腺细胞凋亡及Bcl-2、Bax表达的影响.滨州医学院学报,2011,4(4):249-252.LI Yana,WANG Dong,YIN Yanjun.Effects of exogenous testosterone on thymus cell apoptosis and expression of Bcl-2 and Bax in rats.Journal of Binzhou Medical University,2011,4(4):249-252.
    [5] LORENZEN A,HENDEL J G,CONN K L,et al.Survey of hormone activities in municipal biosolids and animal manures.Environ Toxicol,2004,9(3):216-225.
    [6] 朱威,朱心强.农药的抗雄激素作用及其机制.国外医学:卫生学分册,2005,2(1):14-19.
    [7] 张国军.环境抗雄激素影响及机制.国外医学:卫生学分册,2003,0(2):79-83.
    [8] FENET H,GOMEZ E,PILLON A,et al.Estrogenic activity in water and sediments of a French river:contribution of alkylphenols.Archives of Environmental Contamination and Toxicology,2003,4(1):1-6.
    [9] NOPPE H,VERSLYCKE T,DE WULF E,et al.Occurrence of estrogens in the Scheldt estuary:a 2-year survey.Ecotoxicology and Environmental Safety,2007,6(1):1-8.
    [10] 张国军.环境抗雄激素检测方法.国外医学:卫生学分册,2003,0(2):83-86.
    [11] CHANG H S,CHOO K H,LEE B, et al.The methods of identification, analysis, and removal of endocrine disrupting compounds (EDCs) in water.J Hazard Mater,2009,2(1):1-12.
    [12] SANGSUPAN H A,RADCLIFFE D E,HARTEL P G,et al.Sorption and transport of 17β-estradiol and testosterone in undisturbed soil columns.J Environ Quan,2006,5(6):2261-2272.
    [13] PARKERTON T F.An assessment of the potential environmental risks posed by phthalates in soil and sediment.Series Anthropogenic Compounds:Springer Berlin Heidelberg,2003:317-349.
    [14] ZHAO C,XIE H,ZHANG J,et al.Spatial distribution of organochlorine pesticides(OCPs)and effect of soil characters:a case study of a pesticide producing factory.Chemosphere,2013,0(9):2381-2387.
    [15] KHAN B,LEE L S.Soil temperature and moisture effects on the persistence of synthetic androgen 17alpha-trenbolone,17beta-trenbolone and trendione.Chemosphere,2010,9(8):873-879.
    [16] XIAO R,WANG Z,WANG C,et al.Soil screening for identifying ecological risk stressors using a battery of in vitro cell bioassays.Chemosphere,2006,4(1):71-78.
    [17] WANG Xiaofei,WANG Dianzhong,QIN Xiaofei,et al.Residues of organochlorine pesticides in surface soils from college school yards in Beijing.J Environ Sci,2008,0(9):1090-1096.
    [18] LI J,MA M,GIESY J P,et al.In vitro profling of endocrine disrupting potency of organchlorine pesticides.Toxicology Letters,2008,3:65-71.
    [19] 禹果,肖睿洋,王春霞,等.利用umu/SOS实验评价污灌土壤的遗传毒性.环境科学,2006,7(6):1162-1165.YU Guo,XIAO Ruiyang,WANG Chunxia,et al.Genotoxicity of soil irrigated by wastewater using umu/SOS test.Environmental Science,2006,7(6):1162-1165.
    [20] 庄丽丽,马梅,饶凯峰,等.天津市污水以及再生水处理过程中的雌/孕激素干扰效应.生态毒理学报,2010,5(2):222-228.ZHUANG Lili,MA Mei,RAO Kaifeng,et al.Estrogen and progesterone interference effect of sewage and reclaimed water treatment process in Tianjin.Asian Journal of Ecotoxicology,2010,5(2):222-228.
    [21] LI Jian,WANG Zijian,MA Mei,et al.Analysis of environmental endocrine disrupting activities using recombinant yeast assay in wastewater treatment plant effluents.Bull Environ Contam Toxicol,2010,4:529-535.
    [22] 汪多仁.邻苯二甲酸二(2-丙基庚基)酯的开发与应用.上海塑料,2010,1(3):10-13.WANG Duoren.Development and application of Bis(2-pro pylheptyl) phthalate.Shanghai Plastics,2010,1(3):10-13.
    [23] 陈明,任仁,王子健,等.北京工业废水和城市污水环境激素污染状况调查.环境科学研究,2007,0(6):1-7.CHEN Ming,REN Ren,WANG Zijian,et al.Investigation on status of environmental hormone pollution in the industrial wastewater and urban sewage in Beijing.Research of Environmental Sciences,2007,0(6):1-7.
    [24] CAI Quanying,MO Cehui,WU Qitang,et al.Occurrence of organic contaminants in sewage sludges from eleven wastewater treatment plants,China.Chemosphere,2007,8(9):1751-1762.
    [25] 陆继龙,郝立波,王春珍,等.阎冬第二松花江中下游水体邻苯二甲酸酯分布特征.环境科学与技术,2007,0(12):35-37.LU Jilong,HAO Libo,WANG Chunzhen,et al.Distribution characteristics of phthalic acid esters in middle and lower reaches of No.2 Songhua River.Environmental Science and Technology China,2007,0(12):35-37.
    [26] SUN Ke,JIN Jie,GAO Bo,et al.Sorption of 17 α-ethinyl estradiol, bisphenol A and phenanthrene to different size fractions of soil and sediment.Chemosphere,2012,8(5):577-583.
    [27] HUANG Y Q,WONG C K,ZHENG J S,et al.Bisphenol A(BPA)in China:a review of sources, environmental levels,and potential human health impacts.Environ Intern,2012,2:91-99.
    [28] ZENG Feng,CUI Kunyan,XIE Zhiyong,et al.Phthalate esters(PAEs):emerging organic contaminants in agricultural soils in peri-urban areas around Guangzhou,China.Environ Pollut,2008,6(2):425-434.
    [29] PARIS F,BALAGUER P,TéROUANNE B,et al.Phenylphenols, biphenols, bisphenol-A and 4-tert-octylphenol exhibit α and β estrogen activities and antiandrogen activity in reporter cell lines.Molecular and Cellular Endocrinology,2002,3(1):43-49.
    [30] EMA M.Antiandrogenic effects of dibutyl phthalate and its metabolite,monobutyl phthalate,in rats.Congenit Anom(Kyoto),2002,2:297-308.
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出版历程
  • 收稿日期:  2013-08-19
  • 修回日期:  2013-12-30
  • 刊出日期:  2014-08-25

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