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NO和H2O2对大豆幼苗铝毒害的缓解效应及其相互作用

宋洪明 邢承华 吴 坤 王芳妹 何贞泉 蔡妙珍

宋洪明, 邢承华, 吴 坤, 王芳妹, 何贞泉, 蔡妙珍. NO和H2O2对大豆幼苗铝毒害的缓解效应及其相互作用[J]. 环境科学研究, 2014, 27(9): 1061-1066.
引用本文: 宋洪明, 邢承华, 吴 坤, 王芳妹, 何贞泉, 蔡妙珍. NO和H2O2对大豆幼苗铝毒害的缓解效应及其相互作用[J]. 环境科学研究, 2014, 27(9): 1061-1066.
SONG Hong-ming, XING Cheng-hua, WU Kun, WANG Fang-mei, HE Zhen-quan, CAI Miao-zhen. Alleviation of Aluminum Toxicity in Soybean Seedlings by Nitric Oxide and Hydrogen Peroxide and Analysis of Their Interaction[J]. Research of Environmental Sciences, 2014, 27(9): 1061-1066.
Citation: SONG Hong-ming, XING Cheng-hua, WU Kun, WANG Fang-mei, HE Zhen-quan, CAI Miao-zhen. Alleviation of Aluminum Toxicity in Soybean Seedlings by Nitric Oxide and Hydrogen Peroxide and Analysis of Their Interaction[J]. Research of Environmental Sciences, 2014, 27(9): 1061-1066.

NO和H2O2对大豆幼苗铝毒害的缓解效应及其相互作用

基金项目: 国家自然科学基金项目(31101599);浙江省自然科学基金项目(Y3110561)

Alleviation of Aluminum Toxicity in Soybean Seedlings by Nitric Oxide and Hydrogen Peroxide and Analysis of Their Interaction

  • 摘要: NO和H2O2作为信号分子参与植物对非生物胁迫的响应,为了确定NO和H2O2介导的大豆耐铝作用以及二者的交互作用,以浙春3号大豆为材料,分析了铝毒胁迫下根尖内源及外源NO和H2O2对大豆铝毒的缓解效应,并研究了NO和H2O2代谢相关酶的活性变化, 其中外源NO由SNP(亚硝基铁氰化钠,Na2[Fe(CN)5NO]·2H2O)提供. 结果显示:Al处理显著抑制大豆根生长,促使Al在根尖积累并显著提高大豆根尖内源w(NO)和w(H2O2). 在H2O2+Al、SNP+Al和H2O2+SNP+Al处理下,大豆根相对伸长率比单独Al处理下分别升高了64.6%、63.5%和69.5%,根尖w(Al)则分别减少了34.6%、36.8%和32.9%,表明NO和H2O2对铝毒害均具有缓解作用.外源NO能够促使根尖内源H2O2显著积累,降低POD(过氧化物酶)活性,而外源H2O2对内源根尖w(NO)无影响.NO和H2O2在缓解铝毒的信号途径中存在信号互作,NO处于H2O2的上游,其通过POD活性调节内源w(H2O2).

     

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出版历程
  • 收稿日期:  2013-10-11
  • 修回日期:  2014-01-30
  • 刊出日期:  2014-09-25

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