留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

氮磷对铜绿微囊藻群体形态的影响

周 健 杨桂军 秦伯强 张光生 许慧萍

周 健, 杨桂军, 秦伯强, 张光生, 许慧萍. 氮磷对铜绿微囊藻群体形态的影响[J]. 环境科学研究, 2014, 27(11): 1251-1257.
引用本文: 周 健, 杨桂军, 秦伯强, 张光生, 许慧萍. 氮磷对铜绿微囊藻群体形态的影响[J]. 环境科学研究, 2014, 27(11): 1251-1257.
ZHOU Jian, YANG Gui-jun, QIN Bo-qiang, ZHANG Guang-sheng, XU Hui-ping. Effects of Nitrogen and Phosporous on Colony Formation of Microcystis aeruginosa[J]. Research of Environmental Sciences, 2014, 27(11): 1251-1257.
Citation: ZHOU Jian, YANG Gui-jun, QIN Bo-qiang, ZHANG Guang-sheng, XU Hui-ping. Effects of Nitrogen and Phosporous on Colony Formation of Microcystis aeruginosa[J]. Research of Environmental Sciences, 2014, 27(11): 1251-1257.

氮磷对铜绿微囊藻群体形态的影响

基金项目: 国家水体污染控制与治理科技重大专项(2012ZX07101-010);国家自然科学基金重点项目(41230744);国家自然科学基金项目(41101053)

Effects of Nitrogen and Phosporous on Colony Formation of Microcystis aeruginosa

  • 摘要: 在自然条件下,铜绿微囊藻主要以群体形态存在,其群体形态通常由10~103数量级的单位细胞组成,然而在实验室条件下经过几代培养之后,铜绿微囊藻的群体形态逐渐变为单细胞和少量双细胞. 在实验室条件〔温度为(25±1)℃,光照强度为2 000 lx,光暗比为12 h∶12 h〕下,采用不同ρ(TN)、ρ(TP)的BG11培养基培养群体形态的太湖铜绿微囊藻(FACHB912),其中T1试验组ρ(TN)、ρ(TP)分别为1.00、0.05 mg/L,T2试验组为5.00、0.25 mg/L,T3试验组为25.00、1.25 mg/L,T4试验组为125.00、6.25 mg/L,T5试验组为247.06、7.11 mg/L. 结果表明:T1、T2和T3试验组铜绿微囊藻群体形态细胞所占比例及群体大小均有所增加,T4和T5试验组则表现为减少,并且T1、T2、T3试验组与T4、T5试验组间差异显著(P<0.05). T1、T2、T3试验组出现了>100个细胞的群体,其中T2试验组的铜绿微囊藻群体最大,最大群体约由960个藻细胞组成;而T4、T5试验组中的群体却趋于消失. 相对于单细胞,群体形态的铜绿微囊藻在低ρ(TN)、ρ(TP)条件下能吸收更多的营养盐,有利于细胞的生长;高ρ(TN)、ρ(TP)条件下提供了丰富的营养盐,但可能抑制或者不能刺激胞外多糖的合成和分泌,从而不利于铜绿微囊藻群体形态的维持.

     

  • [1] PAERL H W,OTTEN T G.Harmful cyanobacterial blooms:causes,consequences and controls.Environmental Microbiology,2013,5:995-1010.
    [2] WYNNE T T,STUMPF R P,TOMLINSON M C,et al.Characterizing a cyanobacterial bloom in western Lake Erie using satellite imagery and meteorological data.Limnology Oceanography,2010,5:2025-2036.
    [3] QIN Boqiang,ZHU Guangwei,GAO Guang,et al.A drinking water crisis in Lake Taihu,China:linkage to climatic variability and lake management.Environ Manage,2010,5:105-112.
    [4] BURKERT U,HYENSTRAND P,DRAKARE S,et al.Effects of the mixotrophic flagellate Ochromonas sp.on colony formation in Microcystis aeruginosa.Aquatic Ecology,2001,5:9-17.
    [5] BOLCH J S C,BLACKBUM I S.Isolation and purification of Australian isolates of the toxic cyanobacterium Microcystis aeruginosa Kütz.Journal of Applied Phycology,1996,5:5-13.
    [6] YANG Zhou,KONG Fanxiang,SHI Xiaoli,et al.Morphological response of Microcystis aeruginosa to grazing by different sorts of zooplankton.Hydrobiologia,2006,3:225-230.
    [7] LRLING M,VAN DONK E.Grazer-induced colony formation in Scenedesmus:are there costs to being colonial?.Oikos,2000,8:111-118.
    [8] VAN HOLTHOON F L,VAN BEEK T A,LRLING M,et al.Colony formation in Scenedesmus:a literature overview and further steps towards the chemical characterisation of the Daphnia kairomone.Hydrobiologia,2003,1:241-254.
    [9] GROSSART H P,CZUB G,SIMON M.Algae-bacteria interactions and their effects on aggregation and organic matter flux in the sea.Environmental Microbiology,2006,8:1074-1084.
    [10] SHEN Hong,NIU Yuan,XIE Ping,et al.Morphological and physiological changes in Microcystis aeruginosa as a result of interactions with heterotrophic bacteria.Freshwat Biology,2011,6:1065-1080.
    [11] GAN Nanqin,XIAO Yan,ZHU Lin,et al.The role of microcystins in maintaining colonies of bloom-forming Microcystis spp..Environmental Microbiology,2012,4:730-742.
    [12] KEHR J C,ZILLIGES Y,SPRINGER A.A mannan binding lectin is involved in cell-cell attachment in a toxic strain of Microcystis aeruginosa.Molecular Microbiology,2006,9:893-906.
    [13] SEDMAK B,ELEREK T.Microcystins induce morphological and physiological changes in selected representative phytoplanktons.Microbial Ecology,2005,0:298-305.
    [14] ELLIOTT J A,JONES I D,THACKERAY S J.Testing the sensitivity of phytoplankton communities to changes in water temperature and nutrient load,in a temperate lake.Hydrobiologia,2006,9:401-411.
    [15] LUP F M,FERNANDES H M L,S-CORREIA I,et al.Temperature profiles of cellular growth and exopolysaccharide synthesis by Botryococus braunii Kütz.UC 58.Journal of Applied Phycology,1991,3:35-42.
    [16] MORENO J,VARGAS M A,OLIVARES H,et al.Exopolysaccharide production by the cyanobacterium Anabaena sp.ATCC 33047 in batch and continuous culture.Journal of Biotechnology,1998,0:175-182.
    [17] KOBLIZEK M,KOMENDA J,MASOJIDEK J,et al.Cell aggregation of the cyanobacterium Synechococcus elongatus:role of the electron transport chain.Journal of Phycology,2000,6:662-668.
    [18] QIN Boqiang.A large-scale biological control experiment to improve water quality in eutrophic Lake Taihu,China.Lake Reserv Manage,2013,9:33-46.
    [19] BROOKES J D,CAREY C C.Resilience to blooms.Science,2011,4:46-47.
    [20] DE PHILIPPIS R,VINCENZINI M.Exocellular polysaccharides from cyanobacteria and their possible applications.FEMS Microbiology Reviews,1998,2:151-175.
    [21] PAERL H W,XU Hai,MCCARTHY M J,et al.Controlling harmful cyanobacterial blooms in a hyper-eutrophic lake (Lake Taihu,China):the need for a dual nutrient (N&P) management strategy.Water Res,2011,5:1973-1983.
    [22] VZIE C,RAPALA J,VAITOMAA J,et al.Effect of nitrogen and phosphorus on growth of toxic and nontoxic Microsystis strain and on intracellular microcystin concentrations.Microbial Ecology,2002,3:443-454.
    [23] XU Hai,PAERL H W,QIN Boqiang,et al.Nitrogen and phosphorus inputs control phytoplankton growth in eutrophic Lake Taihu,China.Limnology Oceanography,2010,5:420-432.
    [24] BERMAN T.The role of DON and the effect of N∶P ratios on occurrence of cyanobacterial blooms:implications from the outgrowth of Aphanizomenon in Lake Kinneret.Limnology Oceanography,2001,6:443-447.
    [25] FUJIMOTO N,SUDO R,SUGIURA N.Nutrient-limited growth of Microcystis aeruginosa and Phormidiumtenue and competition under various N∶P supply ratios and temperatures.Limnology Oceanography,1997,2:250-256.
    [26] REYNOLDS C S.What factors influence the species composition of phytoplankton in lakes of different trophic status?.Hydrobiologia,1998,9:11-26.
    [27] XIE Liqiang,XIE Ping,LI Sixin,et al.The low TN∶TP ratio,a cause or a result of Microcystis blooms?.Water Res,2003,7:2073-2080.
    [28] WANG Wei,LIU Ying,YANG Zhou.Combined effects of nitrogen content in media and Ochromonas sp.grazing on colony formation of cultured Microcystis aeruginosa.Journal of Limnology,2010,9:193-198.
    [29] HYENSTRAND P,RYDIN E,GUNNERHED M,et al.Response of the cyanobacterium Gloeotrichia to iron and boron additions:an experiment from Lake Erken.Freshwater Biology,2001,6:735-741.
    [30] RUETER J G,PETERSEN R R.Micronutrient effects on cyanobacterial growth and physiology.New Zealand Journal of Marine Freshwater Research,1987,1:434-435.
    [31] QIN Boqiang,GAO Guang,ZHU Guangwei,et al.Lake eutrophication and its ecosystem response.Chinese Sciehce Bulletin,2013,8:961-970.
    [32] MA Jianrong,BROOKES J D,QIN Boqiang,et al.Environmental factors controlling colony formation in blooms of the cyanobacteria Microcystis spp.in Lake Taihu,China.Harmful Algae,2014,1:136-142.
    [33] YANG Guijun,QIN Boqiang,TANG Xiangming,et al.Contrasting zooplankton communities of two bays of the large,shallow,eutrophic Lake Taihu,China:their relationship to environmental factors.Journal of Great Lakes Research,2012,8:299-308.
    [34] THORNTON D.Diatom aggregation in the sea:mechanisms and ecological.European Journal of Phycology,2002,7:149-161.
    [35] DE PHILIPPIS R,MARGHERI M C,PELOSI E,et al.Exopolysaccharide production by a unicellular cyanobacterium isolated from a hypersaline habitat.Journal of Applied Phycology,1993,5:387-394.
    [36] DE PHILIPPIS R,SILI C,PAPERI R,et al.Exopolysaccharide-producing cyanobacteria and their possible exploitation:a review.Journal of Applied Phycology,2001,3:293-299.
    [37] PAJDAK-STS A,FIALKOWSKA E,FYDA J.Phormidium autumnale (cyanobacteria) defense against three ciliate grazer species.Aquatic Microbial Ecology,2001,3:237-244.
    [38] VAN RIJSSEL M,JANSE I,NOORDKAMP D J B,et al.An inventory of factors that effect polysaccharide production by Phaeocystis globosa.Journal of Sea Research,2000,3:297-306.
    [39] HECKY R E,KILHAM P.Nutrient limitation of phytoplankton in freshwater and marine environments:a review of recent evidence on the effects of enrichment.Limnology Oceanography,1988,3:796-822.
    [40] MAGALETTI E,URBANI R,SIST P,et al.Abundance and chemical characterization of extracellular carbohydrates released by the marine diatom Cylindrotheca fusiformis under N-and P-limitation.European Journal of Phycology,2004,9:133-142.
    [41] GRANUM E,MYKLESTAD S M.A simple combined method for determination of β-1,3-glucan and cell wall polysaccharides in diatoms.Hydrobiologia,2002,7:155-161.
    [42] STAATS N,STAL L J,MUR L R.Exopolysaccharide production by the epipelic diatom Cylindrotheca closterium:effects of nutrient conditions.Journal of Experimental Marine Biology Ecology,2000,9:13-27.
    [43] ARAD S,FRIEDMAN O,ROTEM A.Effect of nitrogen on polysaccharide production in a Porphyridium sp..Appl Environ Microbiol,1988,4:2411-2414.
    [44] GUERRINI F,CANGINI M,BONI L,et al.Metabolic responses of the diatom Achnanthes brevipes (Bacillariophyceae) to nutrient limitation.Journal Phycology,2000,6:882-890.
    [45] CHU Zhaosheng,JIN Xiangcan,YANG Bo,et al.Buoyancy regulation of Microcystis flos:aquae during phosphorus-limited and nitrogen-limited growth.Journal of Plankton Research,2007,9:739-745.
    [46] 阳振.微囊藻群体形成的驱动因子研究.南京:中国科学院南京地理与湖泊研究所,2010:37-38.
    [47] RAI H.The influence of photon flux density (PFD) on short term 14C incorporation into proteins,carbohydrates and lipids in freshwater algae.Hydrobiologia,1995,8:51-59.
    [48] SHEN Hong,SONG Lirong.Comparative studies on physiological responses to phosphorus in two phenotypes of bloom-forming Microcystis.Hydrobiologia,2007,2:475-486.
    [49] WU Zhongxing,GAN Nanqin,HUANG Qun,et al.Response of Microcystis to copper stress:do phenotypes of Microcystis make a difference in stress tolerance?.Environ Pollut,2007,7:324-330.
    [50] WU Zhongxing,SONG Lirong.Physiological comparison between colonial and unicellular forms of Microcystis aeruginosa Kütz.(Cyanobacteria).Phycologia,2008,7:98-104.
  • 加载中
计量
  • 文章访问数:  1773
  • HTML全文浏览量:  10
  • PDF下载量:  85
  • 被引次数: 0
出版历程
  • 收稿日期:  2013-11-21
  • 修回日期:  2014-02-10
  • 刊出日期:  2014-11-25

目录

    /

    返回文章
    返回