留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

非灭菌条件下白腐真菌在反应器中的形态特征变化

周 成 董 明 文湘华

周 成, 董 明, 文湘华. 非灭菌条件下白腐真菌在反应器中的形态特征变化[J]. 环境科学研究, 2014, 27(11): 1373-1378.
引用本文: 周 成, 董 明, 文湘华. 非灭菌条件下白腐真菌在反应器中的形态特征变化[J]. 环境科学研究, 2014, 27(11): 1373-1378.
ZHOU Cheng, DONG Ming, WEN Xiang-hua. Morphological Characteristics of Phanerochaete chrysosporium in a Reactor Operated under Non-Sterile Conditions[J]. Research of Environmental Sciences, 2014, 27(11): 1373-1378.
Citation: ZHOU Cheng, DONG Ming, WEN Xiang-hua. Morphological Characteristics of Phanerochaete chrysosporium in a Reactor Operated under Non-Sterile Conditions[J]. Research of Environmental Sciences, 2014, 27(11): 1373-1378.

非灭菌条件下白腐真菌在反应器中的形态特征变化

基金项目: 国家自然科学基金项目(20677033)

Morphological Characteristics of Phanerochaete chrysosporium in a Reactor Operated under Non-Sterile Conditions

  • 摘要: 揭示白腐真菌(Phanerochaete chrysosporium)在反应器中的形态变化有助于了解白腐真菌反应器难以连续运行问题的本质. 在耦合臭氧单元的反应器对酸性蓝45连续降解的过程中,考察了非固定化和固定化菌丝系统中白腐真菌的形态特性变化. 结果表明,在接种量为1 700 mg/L的非固定化系统中,染菌量为7.1×105 CFU/mL,平均脱色率为19%;但菌丝球形态不稳定,运行8~12 d时出现破裂、菌丝脱落、内容物大量流失,污染微生物的生长加剧. 在固定化系统中,接种量为1 700 mg/L时,染菌量降至8.4×104 CFU/mL,平均脱色率升至22%;接种量为4 300 mg/L时,染菌量降至4.7×104 CFU/mL,平均脱色率达29%. 在固定化菌丝系统中,白腐真菌的形态相对稳定,但21 d后,其稳定性降低,也出现了菌丝脱落现象,这与菌丝球或固定化菌丝的内部菌丝老化和自溶有关,可以考虑通过设计新载体从生物膜的内部和外部同时供给营养物和氧气解决该问题.

     

  • [1] BUMPUS J A,TIEN M,WRIGHT D,et al.Oxidation of persistent environmental pollutants by a white rot fungus.Science,1985,8:1434-1435.
    [2] FIELD J A,DE JONG E,COSTA G F,et al.Biodegradation of polycyclic aromatic hydrocarbons by new isolates of white rot fungi.Appl Environ Microbiol,1992,8:2219-2226.
    [3] PANT D,ADHOLEYA A.Nitrogen removal from bio-methanated spent wash using hydroponic treatment followed by fungal decolorization.Environmental Engineering Science,2009,6:559-565.
    [4] 张晓昱,任拥政,章北平,等.白腐菌-厌氧菌-好氧菌三段式处理蒸煮黑液工艺研究.给水排水,2003,9(11):38-41.
    [5] 李慧蓉.白腐真菌的研究进展.环境科学进展,1996,4(6):71-77.
    [6] REDDYC A.The potential for white-rot fungi in the treatment of pollutants.Environmental Biotechnology,1995,6:320-328.
    [7] 王灿,胡洪营,于茵,等.培养基种类和培养条件对白腐真菌生长和产酶特性的影响.环境科学研究,2007,0(2):9-13.WANG Can,HU Hongying,YU Yin,et al.Influence of culturing mediums and conditions on characterization of growth and enzyme production of white rot fungi.Research of Environmental Sciences,2007,0(2):9-13.
    [8] CERRONE F,BARGHINI P,PESCIAROLI C,et al.Efficient removal of pollutants from olive washing wastewater in bubble-column bioreactor by Trametes versicolor.Chemosphere,2011,4:254-259.
    [9] ENK N,TROT N,ULA M,et al.The use of the fungus Dichomitus squalens for degradation in rotating biological contactor conditions.Bioresource Technology,2012,4:241-246.
    [10] RODARTE-MORALES A I,FEIJOO G,MOREIRA M T,et al.Operation of stirred tank reactors (STRs) and fixed-bed reactors (FBRs) with free and immobilized Phanerochaete chrysosporium for the continuous removal of pharmaceutical compounds.Biochemical Engineering Journal,2012,6:38-45.
    [11] VIPUL R P,NIKHIL S B,BHATT H B.Involvement of ligninolytic enzymes of Myceliophthora vellerea HQ871747 in decolorization and complete mineralization of reactive blue 220.Chemical Engineering Journal,2013,3:98-108.
    [12] CRUZ-MORAT C,FERRANDO-CLIMENT L,RODRIGUEZ-MOZAZ S,et al.Degradation of pharmaceuticals in non-sterile urban wastewater by Trametes versicolor in a fluidized bed bioreactor.Water Res,2013,7:5200-5201.
    [13] GUIMARES C,PORTO P,OLIVEIRA R,et al.Continuous decolourization of a sugar refinery wastewater in a modified rotating biological contactor with Phanerochaete chrysosporium immobilized on polyurethane foam disks.Process Biochemistry,2005,0:535-540.
    [14] BLNQUEZ P,CAMINAL P,MONTSERRAT S,et al.The effect of HRT on the decolourisation of the grey lanaset G textile dye by Trametes versicolor.Chemical Engineering Journal,2007,6:163-169.
    [15] COUTO S R,ROSALES E,SANROMAN M A.Decolourization of synthetic dyes by Trametes hirsute in expanded-bed reactors.Chemosphere,2006,2:1558-1563.
    [16] D'ANNIBALE A,QUARATINO D,FEDERICI F,et al.Effect of agitation and aeration on the reduction of pollutant load of olive mill wastewater by the white-rot fungus Panus tigrinus.Biochemical Engineering Journal,2006,9:243-249.
    [17] DHOUBIB A,ALOUI F,HAMAD N,et al.Pilot-plant treatment of olive mill wastewaters by Phanerochaete chrysosporium coupled to anaerobic digestion and ultrafiltration.Process Biochemistry,2006,1:159-167.
    [18] AGGELIS G,ICONOMOU D,CHRISTOU M,et al.Phenolic removal in a model olive oil mill wastewater using Pleurotus ostreatus in bioreactor cultures and biological evaluation of the process.Water Res,2003,7:3897-3904.
    [19] FUJIT A M,ERA A,IKE M,et al.Decolorization of heat-treatment liquor of waste sludge by a bioreactor using polyurethane foam-immobilized white rot fungus equipped with an ultra-membrane filtration unit.Journal of Bioscience and Bioengineering,2000,0:387-394.
    [20] BORCHERT M,LIBRA J.Decolorization of reactive dyes by the white rot fungus Trametes versicolor in sequencing batch reactors.Biotechnology and Bioengineering,2001,5(3):313-321.
    [21] LIBRA J A,BORCHERT M,BANIT S.Competition strategies for the decolorization of a textile-reactive dye with the white-rot fungi Trametes versicolor under non-sterile conditions.Biotechnology and Bioengineering,2003,2:736-744.
    [22] 周成,文湘华.非灭菌条件下白腐真菌反应器对酸性蓝45的降解特性.环境科学,2009,0(6):235-239.ZHOU Cheng,WEN Xianghua.Degradation of acid blue 45 in a white-rot fungi reactor operated under(nonsterile) conditions.Environmental Science,2009,0(6):235-239.
    [23] ZHOU Cheng,WEN Xianghua,NING Ping.Continuous acid blue 45 decolorization by using a novel open fungal reactor system with ozone as the bactericide.Biochemical Engineering Journal,2013,9:246-252.
    [24] TIEN M,KIRK T K.Lignin peroxidase of Phanerochaete chrysosporium.Methods Enzymol,1988,1:238-248.
    [25] MOREIRA M T,PALMA C,FEIJOO G,et al.Strategies for the continuous production of ligninolytic enzymes in fixed and fluidised bed bioreactors.Journal of Biotechnology,1998,6:27-39.
    [26] 周成,文湘华.打结棉线载体对白腐真菌生长与合成MnP的促进作用.清华大学学报,2009,9(6):846-849,4.ZHOU Cheng,WEN Xianghua.Enhancement of the growth and MnP production of Phanerochaete chrysosporium using knotted cotton-thread carriers.Journal of Tsinghua University,2009,9(6):846-849,4.
    [27] ZHOU Cheng,WEN Xianghua.A novel knotted cotton-thread carrier:its potential use in achieving the biomass renewal of Phanerochaete chrysosporium in an immobilized growth system.Science in China:B,2009,2(5):670-675.
    [28] PASZCZYNSKI A,CRAWFORD R L,HUYNH V B.Manganese peroxidase of Phanerochaete chrysosporium:purification.Methods in Enzymology,1988,1:264-270.
    [29] ROGALSKI J,SZCZODRAK J,JANUSZ G.Manganese peroxidase production in submerged cultures by free and immobilized mycelia of Nematoloma frowardii.Bioresource Technology,2006,7:469-476.
    [30] SAYADI S,ZORGANI F,ELLOUZ R.Decolorization of olive mill waste-waters by free and immobilized Phanerochaete chrysosporium cultures.Applied Biotechnology and Bioengineering,1996,6:265-276.
    [31] LIAO W L,TSENG D H,TSAI YC,et al.Microbial removal of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium.Water Sci Technol,1997,5(8):255-164.
    [32] WHITE S,MCINTYRE M,BERRY D R,et al.The autolysis of industrial filamentous fungi.Critical Reviews in Biotechnology,2002,2(1):1-14.
    [33] MCNEIL B,BERRY D R,HARVEY L M,et al.Measurement of autolysis in submerged batch cultures of Penicilium chrysogenum.Biotechnology and Bioengineering,1998,7:297-305.
    [34] 袁立,许修宏.木耳菌丝老化过程的微形态学研究.菌物学报,2011,0(4):566-571.YUAN Li,XU Xiuhong.Micromorpholgical studies on senescence process of hyphae in cultivated Auricularia auricula-judae.Mycosystema,2011,0(4):566-571.
  • 加载中
计量
  • 文章访问数:  2072
  • HTML全文浏览量:  5
  • PDF下载量:  42
  • 被引次数: 0
出版历程
  • 收稿日期:  2013-11-23
  • 修回日期:  2014-07-20
  • 刊出日期:  2014-11-25

目录

    /

    返回文章
    返回