留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

金雀异黄素对雄性斑马鱼卵黄原蛋白mRNA的诱导机理

王德鑫 陈 洁 王 蔚 汝少国

王德鑫, 陈 洁, 王 蔚, 汝少国. 金雀异黄素对雄性斑马鱼卵黄原蛋白mRNA的诱导机理[J]. 环境科学研究, 2015, 28(1): 46-51.
引用本文: 王德鑫, 陈 洁, 王 蔚, 汝少国. 金雀异黄素对雄性斑马鱼卵黄原蛋白mRNA的诱导机理[J]. 环境科学研究, 2015, 28(1): 46-51.
WANG Dexin, CHEN Jie, WANG Wei, RU Shaoguo. Induction Mechanism of Genistein on Vtg mRNA in Male Zebrafish (Danio rerio)[J]. Research of Environmental Sciences, 2015, 28(1): 46-51.
Citation: WANG Dexin, CHEN Jie, WANG Wei, RU Shaoguo. Induction Mechanism of Genistein on Vtg mRNA in Male Zebrafish (Danio rerio)[J]. Research of Environmental Sciences, 2015, 28(1): 46-51.

金雀异黄素对雄性斑马鱼卵黄原蛋白mRNA的诱导机理

基金项目: 国家自然科学基金项目(31170322)

Induction Mechanism of Genistein on Vtg mRNA in Male Zebrafish (Danio rerio)

  • 摘要: 采用质量浓度分别为1、10、100、1 000 μg/L的金雀异黄素对成年雄性斑马鱼进行水体暴露28 d,通过real-time PCR技术检测雄性斑马鱼肝脏内Vtg-1(卵黄原蛋白)、ERα和ERβ1 mRNA(雌激素受体)的表达水平,并利用同位素放射免疫法检测雄性斑马鱼性腺w(睾酮)和w(E2)(E2为17β-estradiol,17β-雌二醇). 结果表明:10~1 000 μg/L金雀异黄素显著诱导了雄性斑马鱼肝脏Vtg-1 mRNA的表达,二者呈剂量-效应关系,同时显著上调了雌激素受体ERβ1 mRNA的表达;但1~1 000 μg/L金雀异黄素却极显著地抑制了雄性斑马鱼肝脏内ERα mRNA的表达,同时造成雄性斑马鱼性腺w(E2)的升高和w(睾酮)的降低. 研究结果显示,金雀异黄素可能通过提高雄性斑马鱼体内ERβ1mRNA的表达和内源w(E2),增强ERβ1对内源雌激素的介导作用,从而促进Vtg-1的表达.

     

  • [1] 韩锋,张永忠,杨学军.大豆制品中异黄酮各组分含量测定及分析.大豆通报,2006(5):20-23.HAN Feng,ZHANG Yongzhong,YANG Hongjun.The content determination and the analyse of isoflavones component in soy products.Soybean Bulletin,2006(5):20-23.
    [2] 孙君明,孙宝利,韩粉霞,等.快速检测大豆籽粒中十二种异黄酮组分的HPLC方法.中国农业科学,2009,2(7):2491-2498.SUN Junming,SUN Baoli,HAN Fenxia,et al.A rapad HPLC method for determination of 12 isoflavone components in soybean seeds.Scientia Agriculturea Sinica,2009,2(7):2491-2498.
    [3] KIPARISSIS Y,HUGHES R,METCALFE C,et al.Identification of the isoflavonoid genistein in bleached kraft mill effluent.Environmental Science and Technology,2001,5(12):2423-2427.
    [4] KAWANISHI M,TAKAMURA-ENYA T,ERMAWATI R,et al.Detection of genistein as an estrogenic contaminant of river water in Osaka.Environmental Science & Technology,2004,8(23):6424-6429.
    [5] ISHIBASHI H,KOBAYASHI M,KOSHIISHI T,et al.Induction of plasma vitellogenin synthesis by the commercial fish diets in male goldfish(Carassius auratus) and dietary phytoestrogens.Journal of Health Science,2002,8(5):427-434.
    [6] 袁建平,王江海,刘昕.大豆异黄酮生理活性的研究进展:(1)大豆异黄酮的代谢及雌激素特性.中国食品学报,2003,3(3):81-85.YUAN Jianping,WANG Jianghai,LIU Xin.Research advance on the biological activities of soybean isoflavones:(1) metabolism and estrogenic behaviors of soybean isoflavones.Journal of Chinese Institute of Food Science and Technology,2003,3(3):81-85.
    [7] BENNETAU-PELISSERO C,BRETON B B.BENNETAU B,et al.Effect of genistein-enriched diets on the endocrine process of gametogenesis and on reproduction effciency of the rainbow trout Oncorhynchus mykiss.General Comparative Endocrinology,2001,1:173-187.
    [8] SCHOLZ S,KORDES C,HAMANN J,et al.Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes):comparison of gene expression and protein levels.Marine Environmental Research,2004,7:235-244.
    [9] NG Y,HANSON S,MALISON J A,et al.Genistein and other isoflavones found in soybeans inhibit estrogen metabolism in salmomid fish.Aquaculture,2006,4:658-665.
    [10] JOURDEHI A Y,SUDAGAR M,BAHMANI M,et al.Comparative study of dietary soy phytoestrogens genistein and equol effects on growth parameters and ovarian development in farmed female beluga sturgeon,Huso huso.Fish Physiology Biochemistry,2014,0:117-128.
    [11] ZHANG Luling,KHAN I A,FORAN C M.Characterization of the estrogenic response to genistein in Japanese medaka (Oryzias latipes).Comparative Biochemistry Physiology Part C,2002,2:203-211.
    [12] GREEN C C,KELLY A M.Effects of the estrogen mimic genistein as a dietary component on sex differentiation and ethoxyresorufin-O-deethylase (EROD) activity in channel catfish(Ictalurus punctatus).Fish Physiology Biochemistry,2009,5(3):377-384.
    [13] KIPARISSIS Y,BALCH G C,METCALFE T L,et al.Effects of the isoflavones genistein and equol on the gonadal development of Japanese madaka (Oryzias latipes).Environmental Health Perspectives,2003,1(9):1158-1163.
    [14] STEVENSON L M,BROWN A C,MONTGOMERY T M,et al.Reproductive consequences of exposure to waterborne phytoestrogens in male fighting fish Betta splendens.Archives of Environment Contamination and Toxicology,2011,0:501-510.
    [15] TINGAUD-SEQUEIRA A,ANDRE M,FORGUE J,et al.Expression patterns of three estrogen receptor genes during zebrafish(Danio rerio) development:evidence for high expression in neuromasts.Gene Expression Patterns,2004,4:561-568.
    [16] LATONNELLE K,FOSTIER A,MENN F L,et al.Binding affinities of hepatic nuclear estrogen receptors for phytoestrogens in rainbow trout (Oncorhynchus mykiss) and Siberian sturgeon (Acipenser baeri).General and Comparative Endocrinology,2002,9:69-79.
    [17] MENUET A,PAGE Y L,TORRES O,et al.Analysis of the estrogen regulation of the zebrafish estrogen receptor (ER) reveals distinct effects of ERα,ERβ1 and ERβ2.Journal of Molecular Endocrinology,2004,2:975-986.
    [18] TIAN Hua,RU Shaoguo,BING Xin,et al.Effects of monocrotophos on the reproductive axis in the male goldfish (Carassius auratus):potential mechanisms underlying vitellogenin induction.Aquatic Toxicology,2010,8:67-73.
    [19] ARUKWE A,GOKSOYR A.Eggshell and egg yolk proteins in fish:hepatic proteins for the next generation:oogenetic,population,and evolutionary implications of endocrine discription.Comparative Hepatology,2003,2:1-21.
    [20] BARUCCA M,CANAPA A,OLMO E.Analysis of vitellogenin gene induction as a valuable biomarker of estrogenic exposure in various Mediterranean fish species.Environmental Research,2006,1:68-73.
    [21] WANG Zhaoyi,ZHANG Xintian,SHEN Peng,et al.Identification,cloning,expression of human estrogen receptor-α36,a novel variant of human estrogen receptor-α66.Biochemical and Biophysical Research Communications,2005,6:1023-1027.
    [22] JIN Yuanxiang,CHEN Rujia,SUN Liwei,et al.Induction of estrogen-responsive gene transcription in the embryo,larval,juvenile and adult life stages of zebrafish as biomarkers of short-term exposure to endocrine disrupting chemicals.Comparative Biochemistry and Physiology Part C,2009,0(3):414-420.
    [23] SCHILLER V,WICHMANN A,KRIEHUBER R,et al.Studying the effects of genistein on gene expression of fish embryos as an alternative testing approach for endocrine disruption.Comparative Biochemistry and Physiology Part C,2013,7:41-53.
    [24] RIBEIRO C,PARDAL M A,TIRITAN M E,et al.Spatial distribution and quantification of endocrine-distributing chemicals in Sado River Estuary,Portugal.Environmental Monitoring and Assessment,2009,9(1/2/3/4):415-427.
    [25] FERRER I,BARBER L B,THURMAN E M.Gas chromatographic-mass spectrometric fragmentation study of phytoestrogens as their trimethylsiyl derivatives:identification in soy milk and wastewater samples.Journal of Chromatography A,2009,6:6024-6032.
    [26] MENUET A,PELLEGRINI E,ANGLADE I,et al.Molecular characterization of three estrogen receptor forms in zebrafish:binding characteristics,transacctivation properities,and tissue distributions.Biology of Reproduction,2002,6:1881-1892.
    [27] HAWKINS M B,THORNION J W,CREWS D,et al.Identification of a third estrogen receptor and reclassification of estrogen receptors in teleosts.Proceeding of the National Academy of Sciences,2000,7(20):10751-10756.
    [28] COSNEFROY A,BRION F,MAILLOT-MARECHAL E,et al.Selective activation of zebrafish estrogen receptor subtypes by chemicals by using stable receptor gene assay developed in a zebrafish liver cell line.Toxicological Sciences,2012,5(2):439-449.
    [29] SASSI-MESSAI S,GIBERT Y,BERNARD L,et al.The phytoestrogen genistein affects zebrafish development through two different pathways.Plos One,2009,4(3):e4935.1-e4935.12.
    [30] BAGHERI T,IMANPOOR M R,JAFARI V.Effects of diets containing genistein and diadzein in a long-term study on sex steroid dynamics of goldfish(Carassius auratus).Toxicology and Industrial Health,2014,0(2):132-140.
    [31] BENNETAU-PELISSERO C,FLOURIOT G,VALOTAIRE Y,et al.Induction of rainbow trout estradiol receptor (rtER) mRNA and vitellogenin (rtVtg) mRNA by phytoestrogens in hepatocyte cultures.Annals of the New York Academy of Sciences,1998,9:600-601.
  • 加载中
计量
  • 文章访问数:  2060
  • HTML全文浏览量:  15
  • PDF下载量:  3884
  • 被引次数: 0
出版历程
  • 收稿日期:  2014-01-14
  • 修回日期:  2014-05-26
  • 刊出日期:  2015-01-25

目录

    /

    返回文章
    返回