留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

Fe3+对反硝化系统除磷效果及微生物产物的影响

操家顺 谢玉洁 方 芳 胡海兰

操家顺, 谢玉洁, 方 芳, 胡海兰. Fe3+对反硝化系统除磷效果及微生物产物的影响[J]. 环境科学研究, 2015, 28(1): 125-133.
引用本文: 操家顺, 谢玉洁, 方 芳, 胡海兰. Fe3+对反硝化系统除磷效果及微生物产物的影响[J]. 环境科学研究, 2015, 28(1): 125-133.
CAO Jiashun, XIE Yujie, FANG Fang, HU Hailan. Influences of Fe3+ on Phosphorus Removal and Microbial Products in Denitrifying Phosphorus Removal System[J]. Research of Environmental Sciences, 2015, 28(1): 125-133.
Citation: CAO Jiashun, XIE Yujie, FANG Fang, HU Hailan. Influences of Fe3+ on Phosphorus Removal and Microbial Products in Denitrifying Phosphorus Removal System[J]. Research of Environmental Sciences, 2015, 28(1): 125-133.

Fe3+对反硝化系统除磷效果及微生物产物的影响

基金项目: 国家自然科学基金项目(51008112);国家水体污染控制与治理科技重大专项(2012ZX07101-003,2011ZX07301-002)

Influences of Fe3+ on Phosphorus Removal and Microbial Products in Denitrifying Phosphorus Removal System

  • 摘要: 铁盐常作为化学药剂来辅助城市污水处理厂的生物除磷. 利用间歇试验考察投加不同ρ(FeCl3)时反硝化除磷系统中污染物的去除效果以及EPS(胞外聚合物)、PHA(聚羟基脂肪酸酯)、糖原的形成与转化,并通过分析胞内Fe3+含量来解析Fe3+对反硝化除磷系统的影响. 结果表明:①Fe3+投加量(以ρ计)<10 mg/L时,系统中PO43-P的去除率由未投加时的88.4%升至100%;Fe3+投加量>10 mg/L时,PO43-P的去除率随Fe3+投加量的增加而缓慢降至84.4%(Fe3+投加量为25 mg/L时). ②Fe3+投加量(10 mg/L)较低时,会增加污泥中w(总EPS);但由于Fe3+会与EPS中的羟基、氨基等官能团发生络合反应,导致Fe3+投加量(>10 mg/L)较高时可检出的w(总EPS)降低. ③投加Fe3+对厌氧段内w(PHA)、w(糖原)的变化及生物释磷的抑制作用影响不大,但Fe3+投加量(>10 mg/L)较高时对缺氧段NO3--N的生物利用、生物吸磷作用以及PHA和糖原的转化速率有明显的抑制作用. ④缺氧阶段末胞内Fe3+含量(以w计)增加144%(Fe3+投加量为25 mg/L时),说明抑制作用主要是因为缺氧段Fe3+随细胞吸磷作用一并进入胞内,直接影响生物酶活性.

     

  • [1] KUBA E,MURNLEITNEM E,LOOSDRECHT M C M,et al.A metabolic model for the biological phosphorus removal by denitrifying organisms.Biotechnology and Bioengineering,1996,2(6):685-595.
    [2] MEINHOLD J,FILIPE C D M,DAIGGER G T,et al.Characterization of the denitrify fraction of phosphate accumulating organisms in biological phosphate removal.Water Science and Technology,1999,9(1):31-42.
    [3] KERRN-JESPERSEN J P,HENZE M.Biological phosphorus uptake under anoxic and aerobic conditions.Water Research,1993,7(4):617-624.
    [4] 邓仁建,张金松,曲志军.污泥浓度对双重后置反硝化工艺脱氮除磷的影响.环境科学研究,2014,7(7):797-803.DENG Renjian,ZHANG Jinsong,QU Zhijun.Characteristics of nitrogen and phosphorus removal in multiple post-denitrification processes with different sludge concentrations.Research of Environmental Sciences,2014,7(7):797-803.
    [5] 华清秀,朱光灿,袁俊,等.多点交替进水阶式A2/O工艺氮磷去除途径.环境科学研究,2014,7(7):749-757.HUA Qingxiu,ZHU Guangcan,YUAN Jun,et al.Phosphorus and nitrogen removal pathways in a commutative multi-influent cascade A2/O process.Research of Environmental Sciences,2014,7(7):749-757.
    [6] 冯华军,胡立芳,邱才娣,等.3类金属离子对活性污泥吸附水体腐殖酸的影响.环境科学,2008,9(1):77-81.FENG Huajun,HU Lifang,QIU Caidi,et al.Influence on three categories of metal ions on adsorption of humic acid by activated sludge.Environmental Science,2008,9(1):77-81.
    [7] 唐金花,许国仁,萧静,等.Fe2(SO4)3对活性微生物活性的影响.哈尔滨工业大学学报,2012,4(6):1-5.TANG Jinhua,XU Guoren,XIAO Jing,et al.The effect of Fe2(SO4)3 on microbial activity of activated sludge.Journal of Harbin Institute of Technology,2012,4(6):1-5.
    [8] 李久义,吴晓清,陈福泰,等.Fe3+对活性污泥絮体结构和生物絮凝作用的影响.环境科学学报,2003,3(5):582-587.LI Jiuyi,WU Xiaoqing,CHEN Futai,et al.Effect of Fe(Ⅲ) on floc surface properties and bioflocculation of activated sludge.Acta Scientiae Circumstantiae,2003,3(5):582-587.
    [9] 夏雪,邵明非,吕小梅,等.不同碳源驯化除磷污泥的除磷效果及菌群结构分析.环境科学研究,2014,7(8):936-942.XIA Xue,SHAO Mingfei,LYU Xiaomei,et al.Phosphorus removal performance and bacterial community structure of denitrifying phosphorus removal sludge operated with different carbon sources.Research of Environmental Sciences,2014,7(8):936-942.
    [10] 操家顺,肖敏艳,方芳,等.不同碳源培养的反硝化聚磷菌对电子受体适应性研究.河海大学学报(科学自然版),2013,1(3):211-217.CAO Jiashun,XIAO Minyan,FANG Fang,et al.Study on the adaptability of DPB cultivated in different carbon source conditions to different electron acceptors.Journal of Hohai University(Natural Sciences),2013,1(3):211-217.
    [11] 国家环境保护总局.水和废水监测分析方法.4版.北京:中国环境科学出版社,2002.
    [12] LIANG Zhiwei,LI Wenhong,YANG Shangyuan,et al.Extraction and structural characteristics of extracellular polymeric substances (EPS),pellets in autotrophic nitrifying biofilm and activated sludge.Chemosphere,2010,1(5):626-632.
    [13] LOWRY O H,FARR A L,RANDALL R J.Protein measurement with the folin phenol reagent.Journal of Biological Chemistry,1951,3(1):265-275.
    [14] DUBOIS M,GILLES K A,HAMILTON J K,et al.Colorimetric method for determination of sugars and related substance.Analytical Chemistry,1956,8(3):350-356.
    [15] 李夕耀,彭永臻,王淑莹,等.聚磷菌胞内多聚物的分析检测方法.四川环境,2009,8(2):106-111.LI Xiyao,PENG Yongzhen,WANG Shuying,et al.Analytical method for intracellular polymers of polyphosphate accumulating organisms.Sichuan Environment,2009,8(2):106-111.
    [16] HU Zhiqiang,CHANDRAN K,GRASSO D,et al.Impact of metal sorption and internalization on nitrification inhibition.Environmental Science & Technology,2003,7(4):728-734.
    [17] MURTHY S N,NOVAK J T,DE HAAS R D.Monitoring cation to predict and improve activated sluge sluge settling and dewatering properties of industrial wastewater.Water Science and Technology,1998,8(3):119-126.
    [18] 姚婧梅,张智,任丽平,等.化学除磷中Fe(Ⅲ)和Fe(Ⅱ)对活性污泥系统的影响.中国给水排水,2012,8(9):20-25.YAO Jingmei,ZHANG Zhi,REN Liping,et al.Effects of Fe(Ⅲ) salt and Fe(Ⅱ) salt on activated sludge system during chemical phosphorus removal.China Water & Wastewater,2012,8(9):20-25.
    [19] LIU Yancheng,SHI Hanchang,LI Wenlin,et al.Inhibition of chemical dose in biological phosphorus and nitrogen removal in simultaneous chemical precipitation for phosphorus removal.Bioresource Technology,2011,2(5):4008-4012.
    [20] ACEVEDO B,OEHMEN A,CARVALHO G,et al.Metabolic shift of polyphosphate-accumulating organisms with different levels of polyphosphate storage.Water Research,2012,6(6):1889-1900.
    [21] BASSIN J P,KLEEREBEZEM R,DEZOTTI M,et al.Simultaneous nitrogen and phosphate removal in aerobic granular sludge reactors operated at different temperatures.Water Research,2012,6(12):3805-3816.
    [22] 倪丙杰,徐得潜,刘绍根,等.污泥性质的重要影响物质胞外聚合物(EPS).环境科学与技术,2006,9(3):108-110.NI Bingjie,XU Deqian,LIU Shaogen,et al.Extracellular polymeric substance (EPS) and its influence on properties of activated sludge.Environmental Science & Technology(China),2006,9(3):108-110.
    [23] 康福星,龙健,王倩,等.微生物胞外聚合物对水体重金属和富营养元素的环境生化效应研究展望.应用与环境生物学报,2010,6(1):129-134.KANG Fuxing,LONG Jian,WANG Qian,et al.Environmental & biochemical effects of MiCrobial extracellular polymeric substances on the heavy metals and entrophic elements in water areas:a review.Chinese Journal of Applied and Environmental Biology,2010,6(1):129-134.
    [24] 傅平青.水环境中的溶解有机质及其与金属离子的相互作用:荧光光谱学研究.北京:中国科学院研究生院,2004.
    [25] 王强.腐殖酸与铁锰铝及其氧化物的相互作用机理研究.重庆:西南农业大学,2005.
    [26] CHEN J H,LION W,GHIORSE W C,et al.Mobilization of adsorbed cadmium and lead in aquifer material by bacterial extracellular polymers.Water Research,1995,9(2):421-30.
    [27] SHENG Guoping,XU Juan,LI Weihua,et al.Quantification of the interactions between Ca2+,Hg2+ and extracellular polymeric substances (EPS) of sludge.Chemosphere,2013,3(7),1436-1441.
    [28] CHEN Wen,WESTERHOFF P,LEENHEER J A,et al.Fluorescence excitation:emission matrix regional integration to quantify spectra for dissolved organic matter.Environmental Science & Technology,2003,7(24):5701-5710.
    [29] ZHANG Daoyong,LEE D J,PAN Xiangliang.Fluorescent quenching for biofilm extracellular polymeric substances (EPS) bound with Cu(Ⅱ).Journal of the Taiwan Institute of Chemical Engineers,2012,3(3):450-454.
    [30] WANG Yayi,GENG Junjun,PENG Yongzhen,et al.A comparison of endogenous processes during anaerobic starvation in anaerobic end sludge and aerobic end sludge from an anaerobic/anoxic/oxic sequencing batch reactor performing denitrifying phosphorus removal.Bioresource Technology,2012,4:19-27.
    [31] 肖凡,顾国维.生物强化除磷机理的探讨.环境科学与技术,2005,8(S1):7-9.
    [32] WANG Yayi,REN Zhongjia,JIANG Fan,et al.Effect of copper ion on the anaerobic and aerobic metabolism of phosphorus accumulating organisms linked to intracellular storage compounds.Journal of Hazardous Materials,2011,6(1):313-319.
    [33] YOU Shengjie,TSAI Yungpin,CHO Bochuan,et al.Metabolic influence of lead on polyhydroxyalkanoates (PHA) production and phosphate uptake in activated sludge fed with glucose or acetic acid as carbon source.Bioresource Technology,2011,2(17):8165-8170.
    [34] LEDIN M.Accumulation of metals by microorganisms:processes and importance for soil systems.Earth-Science Reviews,2000,1(1/2/3/4):1-31.
    [35] BOSWELL C D,DICK R E,ECCLES H,et al.Phosphate uptake and release by Acinetobacter johnsonii in continuous culture and coupling of phosphate release to heavy metal accumulation.Journal of Industrial Microbiology and Biotechnology,2001,6(6):333-340.
  • 加载中
计量
  • 文章访问数:  2272
  • HTML全文浏览量:  9
  • PDF下载量:  3915
  • 被引次数: 0
出版历程
  • 收稿日期:  2014-01-09
  • 修回日期:  2014-08-06
  • 刊出日期:  2015-01-25

目录

    /

    返回文章
    返回