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城市河流异养菌耐药状况及微生物群落结构特征

张崇淼 王 燕 杜 聪

张崇淼, 王 燕, 杜 聪. 城市河流异养菌耐药状况及微生物群落结构特征[J]. 环境科学研究, 2015, 28(5): 713-719.
引用本文: 张崇淼, 王 燕, 杜 聪. 城市河流异养菌耐药状况及微生物群落结构特征[J]. 环境科学研究, 2015, 28(5): 713-719.
ZHANG Chongmiao, WANG Yan, DU Cong. Antibiotic Resistance of Heterotrophic Bacteria and Characteristics of Microbial Community Structure in Urban Rivers in China[J]. Research of Environmental Sciences, 2015, 28(5): 713-719.
Citation: ZHANG Chongmiao, WANG Yan, DU Cong. Antibiotic Resistance of Heterotrophic Bacteria and Characteristics of Microbial Community Structure in Urban Rivers in China[J]. Research of Environmental Sciences, 2015, 28(5): 713-719.

城市河流异养菌耐药状况及微生物群落结构特征

基金项目: 国家自然科学基金项目(51248002);陕西省污水处理与资源化重点科技创新团队项目(2013KCT-13);国家科技支撑计划项目(2014BAC13B06)

Antibiotic Resistance of Heterotrophic Bacteria and Characteristics of Microbial Community Structure in Urban Rivers in China

  • 摘要: 为深入了解城市河流中细菌的耐药状况和微生物群落结构特征,在西安市浐河和灞河河段8个采样点以及某污水处理厂二级出水排放口采集水样,利用细菌培养技术、PCR检测和DGGE(变性梯度凝胶电泳)分析技术,对河流中异养菌耐药率、四环素抗性基因以及微生物群落结构进行研究. 结果表明:浐河和灞河水样中异养菌对SMZ(磺胺甲恶唑)、TET(四环素)、CIP(环丙沙星)和CTX(头孢噻肟)的平均耐药率分别为18.3%、6.2%、2.7%和1.3%,耐药率高低顺序与污水厂排放水中的异养菌相同. 城市河流中异养菌浓度与其耐药率并不存在显著的相关性. 与上游河段相比,污水厂排放口下游河段的异养菌浓度没有明显增加,但对多种抗生素的耐药率却有所升高. 浐河和灞河中四环素抗性基因主要是tetA和tetB,检出率分别为100%和75%,这意味着城市河流中异养菌对TET的耐药性可能主要通过编码外输泵蛋白的抗性机制来实现. 污水厂排放水进入城市河流,并未显著影响河流中的微生物群落结构,但却使某些原有的敏感物种消失,而病原菌或条件致病菌在邻近污水厂排放口的下游河段则有所增加.

     

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  • 刊出日期:  2015-05-25

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