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抗生素和铜联合作用对蚕豆根尖细胞微核率的影响

赵 祥 王金花 朱鲁生 王 军

赵 祥, 王金花, 朱鲁生, 王 军. 抗生素和铜联合作用对蚕豆根尖细胞微核率的影响[J]. 环境科学研究, 2015, 28(7): 1085-1090.
引用本文: 赵 祥, 王金花, 朱鲁生, 王 军. 抗生素和铜联合作用对蚕豆根尖细胞微核率的影响[J]. 环境科学研究, 2015, 28(7): 1085-1090.
ZHAO Xiang, WANG Jinhua, ZHU Lusheng, WANG Jun. Influence of Antibiotics and Copper on Micronucleus Rate of Root-Tip Cells of Vicia-faba[J]. Research of Environmental Sciences, 2015, 28(7): 1085-1090.
Citation: ZHAO Xiang, WANG Jinhua, ZHU Lusheng, WANG Jun. Influence of Antibiotics and Copper on Micronucleus Rate of Root-Tip Cells of Vicia-faba[J]. Research of Environmental Sciences, 2015, 28(7): 1085-1090.

抗生素和铜联合作用对蚕豆根尖细胞微核率的影响

基金项目: 国家自然科学基金项目(21377075);环境污染过程与基准教育部重点实验室(南开大学)开放课题基金项目;山东省自然科学基金项目(ZR2013DQ007)

Influence of Antibiotics and Copper on Micronucleus Rate of Root-Tip Cells of Vicia-faba

  • 摘要: 为确定抗生素与重金属单一及联合作用对细胞遗传物质的损伤程度,以青皮蚕豆根尖细胞为试验材料,研究四环素类抗生素OTC(土霉素)、CTC(金霉素)、TTC(四环素)和重金属Cu单一以及联合作用对蚕豆根尖细胞微核率和微核指数的影响. 结果表明:①抗生素与Cu单独作用,二者c在0~1.6 mmol/L之间时,蚕豆根尖细胞微核率均高于3.33‰,微核指数均大于1.5,与CK(对照处理)相比差异显著(P<0.01);随着c(抗生素)的增大,蚕豆根尖细胞微核率呈先减后增的趋势. ②抗生素与Cu联合作用时,蚕豆根尖细胞微核率和微核指数均小于单独作用时二者之和,交互作用表现为拮抗作用,其中c(抗生素)∶c(Cu)为1∶4时拮抗作用最明显,c(抗生素)∶c(Cu)为1∶1和1∶2时根尖细胞微核率呈减少的趋势,c(抗生素)∶c(Cu)为1∶8和1∶16时根尖细胞微核率呈增加的趋势. 研究显示,四环素类抗生素与Cu单一及联合作用于蚕豆根尖细胞时,均会对植物根尖细胞造成遗传损伤,但是联合作用时,抗生素在溶液中与Cu发生络合反应,从而可以在一定程度上降低对根尖细胞的遗传损伤.

     

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  • 刊出日期:  2015-07-25

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