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双酚AF暴露对胚胎期和幼鱼期斑马鱼的毒性效应

杨 洋 陈亚文 唐天乐 唐文浩

杨 洋, 陈亚文, 唐天乐, 唐文浩. 双酚AF暴露对胚胎期和幼鱼期斑马鱼的毒性效应[J]. 环境科学研究, 2015, 28(8): 1219-1226.
引用本文: 杨 洋, 陈亚文, 唐天乐, 唐文浩. 双酚AF暴露对胚胎期和幼鱼期斑马鱼的毒性效应[J]. 环境科学研究, 2015, 28(8): 1219-1226.
YANG Yang, CHEN Yawen, TANG Tianle, TANG Wenhao. Toxic Effects of Bisphenol AF on Zebrafish Embryos and Larvae[J]. Research of Environmental Sciences, 2015, 28(8): 1219-1226.
Citation: YANG Yang, CHEN Yawen, TANG Tianle, TANG Wenhao. Toxic Effects of Bisphenol AF on Zebrafish Embryos and Larvae[J]. Research of Environmental Sciences, 2015, 28(8): 1219-1226.

双酚AF暴露对胚胎期和幼鱼期斑马鱼的毒性效应

基金项目: 海南省中西部高校提升综合实力工作资金资助项目(ZXBJH-XK004)

Toxic Effects of Bisphenol AF on Zebrafish Embryos and Larvae

  • 摘要: 为明确不同发育阶段的斑马鱼对BPAF(双酚AF)暴露的易感性,采用暴露试验法初步研究了BPAF对胚胎期和幼鱼期斑马鱼的发育毒性. 结果表明:①BPAF暴露可延缓胚胎期斑马鱼的发育和孵化,使其出现心包水肿、卵黄囊异常、心率下降、心脏搏动停止等症状. ②暴露96 h后,ρ(BPAF)为2.0、2.5、3.0 mg/L暴露组胚胎期斑马鱼的畸形率高达100%,暴露24 h致畸的EC50(半数效应浓度)为2.00 mg/L,暴露96 h的LC50(半数致死浓度)为1.84 mg/L. ③暴露72 h后,对照组幼鱼期斑马鱼鱼鳔发育缺陷率为0;除ρ(BPAF)为1.0 mg/L暴露组外,其余BPAF暴露组幼鱼期斑马鱼鱼鳔发育缺陷率为100%. ④随着暴露时间的延长以及中毒程度的加深,幼鱼期斑马鱼出现的中毒症状依次表现为心包水肿、卵黄囊水肿、背脊弯曲、心跳停止,暴露48和72 h时其心包水肿的EC50分别为1.76、1.56 mg/L,暴露96 h的LC50为1.77 mg/L. 胚胎期和幼鱼期斑马鱼对BPAF暴露响应的差异分析显示,幼鱼期斑马鱼对BPAF暴露的反应更为敏感,幼鱼期斑马鱼的心包水肿症状可作为BPAF毒性响应的最佳指标之一.

     

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  • 刊出日期:  2015-08-25

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