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反硝化除磷系统中PHB红外光谱解析及其与磷去除率的相关性

张 华 朱 菁 宋 箭 黄 健 张 勇 王 宽 黄 珊

张 华, 朱 菁, 宋 箭, 黄 健, 张 勇, 王 宽, 黄 珊. 反硝化除磷系统中PHB红外光谱解析及其与磷去除率的相关性[J]. 环境科学研究, 2015, 28(8): 1274-1280.
引用本文: 张 华, 朱 菁, 宋 箭, 黄 健, 张 勇, 王 宽, 黄 珊. 反硝化除磷系统中PHB红外光谱解析及其与磷去除率的相关性[J]. 环境科学研究, 2015, 28(8): 1274-1280.
ZHANG Hua, ZHU Jing, SONG Jian, HUANG Jian, ZHANG Yong, WANG Kuan, HUANG Shan. Infrared Spectrum Analysis of PHB and the Correlation between PHB Reduction and Orthophosphate Removal Rate during Denitrifying Phosphorus Removal[J]. Research of Environmental Sciences, 2015, 28(8): 1274-1280.
Citation: ZHANG Hua, ZHU Jing, SONG Jian, HUANG Jian, ZHANG Yong, WANG Kuan, HUANG Shan. Infrared Spectrum Analysis of PHB and the Correlation between PHB Reduction and Orthophosphate Removal Rate during Denitrifying Phosphorus Removal[J]. Research of Environmental Sciences, 2015, 28(8): 1274-1280.

反硝化除磷系统中PHB红外光谱解析及其与磷去除率的相关性

基金项目: 国家水体污染控制与治理科技重大专项(2014ZX07303003-09);安徽省科技攻关计划项目(1301032137-04);安徽建筑大学博士基金项目(2013-6)

Infrared Spectrum Analysis of PHB and the Correlation between PHB Reduction and Orthophosphate Removal Rate during Denitrifying Phosphorus Removal

  • 摘要: 为快速、准确测定生物除磷系统中微生物细胞内w(PHB)(PHB为poly-β-hydroxy butyrate,聚-β-羟基丁酸酯)并及时预测PO43--P去除率,采用红外光谱法对微生物细胞内PHB的转化规律进行解析,并结合气相色谱法建立w(PHB)的快速测定模型,研究w(PHB)减少量与PO43--P去除率的相关性. 结果表明:反硝化除磷系统中微生物细胞内PHB的特征峰位于波数1 730 cm-1处,PHB特征峰的吸光度与w(PHB)的增减趋势相同. 采用PLS(partial least squares,偏最小二乘法)对反硝化除磷系统中微生物细胞内PHB特征峰吸光度与w(PHB)建立PLS模型,对w(PHB)的校正和预测时的相关系数(R2)分别达到0.963 0、0.951 4,其均方根误差分别为0.031 0、0.037 9. 根据预测结果,进一步建立了反硝化除磷缺氧段w(PHB)减少量与PO43--P去除率的相关关系,相关系数为0.979 0. 研究表明,利用PLS模型对反硝化除磷系统中PHB红外光谱解析能够快速、准确地表征反硝化除磷系统中微生物细胞内w(PHB)的变化,并能进一步预测PO43--P去除率.

     

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