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浑河底泥反硝化厌氧甲烷氧化菌群落结构时空特征

王 佳 宋永会 彭剑峰 袁林江

王 佳, 宋永会, 彭剑峰, 袁林江. 浑河底泥反硝化厌氧甲烷氧化菌群落结构时空特征[J]. 环境科学研究, 2015, 28(11): 1670-1676.
引用本文: 王 佳, 宋永会, 彭剑峰, 袁林江. 浑河底泥反硝化厌氧甲烷氧化菌群落结构时空特征[J]. 环境科学研究, 2015, 28(11): 1670-1676.
WANG Jia, SONG Yonghui, PENG Jianfeng, YUAN Linjiang. Spatial-Temporal Distribution and Community Structure Analysis of Nitrite-Dependent Anaerobic Methane-Oxidizing Bacteria in the Sediments of the Hun River[J]. Research of Environmental Sciences, 2015, 28(11): 1670-1676.
Citation: WANG Jia, SONG Yonghui, PENG Jianfeng, YUAN Linjiang. Spatial-Temporal Distribution and Community Structure Analysis of Nitrite-Dependent Anaerobic Methane-Oxidizing Bacteria in the Sediments of the Hun River[J]. Research of Environmental Sciences, 2015, 28(11): 1670-1676.

浑河底泥反硝化厌氧甲烷氧化菌群落结构时空特征

基金项目: 国家水体污染控制与治理科技重大专项(2012ZX07202-005)

Spatial-Temporal Distribution and Community Structure Analysis of Nitrite-Dependent Anaerobic Methane-Oxidizing Bacteria in the Sediments of the Hun River

  • 摘要: 为了解在反硝化厌氧甲烷氧化反应过程中起主要作用的微生物——Candidatus Methylomirabilis oxyfera(M. oxyfera)在河流底泥环境中的时空变化特征,利用克隆文库及实时荧光定量PCR技术考察了浑河流域部分底泥中M. oxyfera-like的16S rRNA和pmoA功能基因的多样性及丰度. 结果表明:从浑河上游至下游丰水期各采样点底泥中M. oxyfera-like的16S rRNA的多样性无明显地域性差异,其OTU(operational taxonomic unit,独立操作单元)数变幅不大;pmoA功能基因的多样性则存在较为明显的地域性差异,上游采样点A(章党大桥)底泥中OTU数为5,下游采样点E(三岔河大桥)底泥中仅为1. 采样点A底泥中M. oxyfera-like的16S rRNA和pmoA功能基因的多样性均存在一定的季节性差异,平水期16S rRNA和pmoA功能基因的Shannon-Wiener多样性指数分别为1.48和2.19,在各期中最高;而枯水期时二者均最低,分别为0.83和1.26. 丰度分析结果显示,所有采样点底泥中M. oxyfera-like的16S rRNA丰度均表现为平水期(2.42×106~1.74×107 copies/g)(以干质量计)最高、枯水期(2.87×104~4.19×106 copies/g)居中、丰水期(1.29×103~2.04×104 copies/g)最低,说明浑河底泥中M. oxyfera-like的丰度亦存在一定的季节性差异.

     

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出版历程
  • 收稿日期:  2015-03-27
  • 修回日期:  2015-07-14
  • 刊出日期:  2015-11-25

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