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芦苇酶解对氢气-甲烷联产过程微生物群落演替规律的影响

贾璇 任连海 李鸣晓 席北斗 祝超伟

贾璇, 任连海, 李鸣晓, 席北斗, 祝超伟. 芦苇酶解对氢气-甲烷联产过程微生物群落演替规律的影响[J]. 环境科学研究, 2016, 29(1): 138-145.
引用本文: 贾璇, 任连海, 李鸣晓, 席北斗, 祝超伟. 芦苇酶解对氢气-甲烷联产过程微生物群落演替规律的影响[J]. 环境科学研究, 2016, 29(1): 138-145.
JIA Xuan, REN Lianhai, LI Mingxiao, XI Beidou, ZHU Chaowei. Effects of Enzymatic Pretreatment on Microbial Community Succession in Hydrogen and Methane Coproduction from Reed Straw[J]. Research of Environmental Sciences, 2016, 29(1): 138-145.
Citation: JIA Xuan, REN Lianhai, LI Mingxiao, XI Beidou, ZHU Chaowei. Effects of Enzymatic Pretreatment on Microbial Community Succession in Hydrogen and Methane Coproduction from Reed Straw[J]. Research of Environmental Sciences, 2016, 29(1): 138-145.

芦苇酶解对氢气-甲烷联产过程微生物群落演替规律的影响

基金项目: 国家自然科学基金项目(21406213,51408572)

Effects of Enzymatic Pretreatment on Microbial Community Succession in Hydrogen and Methane Coproduction from Reed Straw

  • 摘要: 采用10 mg/g纤维素酶R-10预处理芦苇秸秆,研究了酶解预处理对芦苇厌氧产气潜力的影响,分析了氢气-甲烷联产过程中微生物群落结构演替规律. 结果表明:酶解预处理后,芦苇秸秆在产氢阶段的累积产气量和φ(H2)分别达到42.5 mL/g和52.1%;在产甲烷阶段,累积产气量稳定上升,最高值可达137.5 mL/g,是对照组产气量的5倍. 由扫描电镜(SEM)观察可知,产氢阶段以短杆状和梭状菌为主,产甲烷阶段以长杆菌为主. PCR-DGGE(聚合酶链式反应-变性梯度凝胶电泳)分析表明,芦苇在酶解预处理后,其厌氧联产过程中微生物群落呈规律性演替,产氢阶段的优势微生物分别为具有降解纤维素产氢气功能的嗜热纤维素菌Clostridium thermocellum(条带B20)、具有高效产氢潜力的兼性厌氧产气肠杆菌Enterobacter aerogenes(条带B28);在产甲烷阶段,其优势微生物为可利用氢营养途径合成甲烷的产甲烷古菌Methanoculleus bourgensis(条带A3)、Methanoculleus horonobensis(条带A13). 经纤维素酶预处理后,芦苇秸秆厌氧联产的累积产气量、φ(H2) 提高显著,具有纤维素降解功能的细菌和可利用氢营养途径合成甲烷的古菌为主要优势微生物.

     

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出版历程
  • 收稿日期:  2015-06-09
  • 修回日期:  2015-08-26
  • 刊出日期:  2016-01-25

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