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低氧条件下不同电子受体对克雷伯氏菌降解菲的影响

侯晓鹏 叶春 李春华 郑向勇 许士洪 郭士林

侯晓鹏, 叶春, 李春华, 郑向勇, 许士洪, 郭士林. 低氧条件下不同电子受体对克雷伯氏菌降解菲的影响[J]. 环境科学研究, 2016, 29(2): 227-233.
引用本文: 侯晓鹏, 叶春, 李春华, 郑向勇, 许士洪, 郭士林. 低氧条件下不同电子受体对克雷伯氏菌降解菲的影响[J]. 环境科学研究, 2016, 29(2): 227-233.
HOU Xiaopeng, YE Chun, LI Chunhua, ZHENG Xiangyong, XU Shihong, GUO Shilin. Effects of Different Electron Acceptors on the Biodegradation of Phenanthrene by Klebsiella sp. ZS1 under Low-Oxygen Condition[J]. Research of Environmental Sciences, 2016, 29(2): 227-233.
Citation: HOU Xiaopeng, YE Chun, LI Chunhua, ZHENG Xiangyong, XU Shihong, GUO Shilin. Effects of Different Electron Acceptors on the Biodegradation of Phenanthrene by Klebsiella sp. ZS1 under Low-Oxygen Condition[J]. Research of Environmental Sciences, 2016, 29(2): 227-233.

低氧条件下不同电子受体对克雷伯氏菌降解菲的影响

基金项目: 国家自然科学基金项目(41101487);浙江省亚热带水环境与海洋生物资源保护重点实验室开放基金项目

Effects of Different Electron Acceptors on the Biodegradation of Phenanthrene by Klebsiella sp. ZS1 under Low-Oxygen Condition

  • 摘要: 为考察低氧条件下不同电子受体对于克雷伯氏菌(Klebsiella sp.ZS1,下称ZS1)降解菲的影响,在8%氧分压下,分别添加20 mmol/L Na2SO4、20 mmol/L NaNO3、10 mmol/L FeCl3为电子受体进行降解菌的培养. 通过分光光度法和平皿计数法分别测定电子受体消耗率和菌体生长量,并采用气-质联用法(GC-MS)测定ρ(菲),对不同电子受体影响下的菌体生长量和ρ(菲)进行单因素方差分析. 结果表明,在低氧环境下ZS1降解菲过程中,SO42-、NO3-、Fe3+的消耗率分别为74.7%、0.2%、4.5%;电子转移速率分别为1 899、0.366 3、7.679 μmol/d. 未接种ZS1时,ρ(菲)只减少了10.1%;接种ZS1后,不添加电子受体和分别添加SO42-、NO3-、Fe3+下菲的降解率分别为68.9%、86.2%、72.9%和68.5%,一级动力学方程求得的降解速率常数分别为0.181、0.360、0.186、0.183 d-1. 添加SO42-组ZS1的生长量是不添加电子受体组的2.5倍,而添加NO3-或Fe3+时与不添加电子受体组基本相等. 研究显示,在低氧条件下,ZS1降解菲过程中可同时利用SO42-和O2为电子受体;添加SO42-作为外源电子受体对ZS1的生长及降解能力有很强的促进作用;而添加NO3-和添加Fe3+对ZS1降解菲和ZS1的生长没有显著影响.

     

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  • 收稿日期:  2015-09-05
  • 修回日期:  2015-11-24
  • 刊出日期:  2016-02-25

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