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青霉素菌渣堆肥过程中青霉素钠降解菌的分离与鉴定

赵娟 张振华 段会英 余冉 刘燕 王长永

赵娟, 张振华, 段会英, 余冉, 刘燕, 王长永. 青霉素菌渣堆肥过程中青霉素钠降解菌的分离与鉴定[J]. 环境科学研究, 2016, 29(2): 271-278.
引用本文: 赵娟, 张振华, 段会英, 余冉, 刘燕, 王长永. 青霉素菌渣堆肥过程中青霉素钠降解菌的分离与鉴定[J]. 环境科学研究, 2016, 29(2): 271-278.
ZHAO Juan, ZHANG Zhenhua, DUAN Huiying, YU Ran, LIU Yan, WANG Changyong. Isolation and Identification of a Penicillin-Degrading Strain during Composting of Penicillin Bacteria Residue[J]. Research of Environmental Sciences, 2016, 29(2): 271-278.
Citation: ZHAO Juan, ZHANG Zhenhua, DUAN Huiying, YU Ran, LIU Yan, WANG Changyong. Isolation and Identification of a Penicillin-Degrading Strain during Composting of Penicillin Bacteria Residue[J]. Research of Environmental Sciences, 2016, 29(2): 271-278.

青霉素菌渣堆肥过程中青霉素钠降解菌的分离与鉴定

基金项目: 国家自然科学基金青年科学基金项目(41401363);江苏省自然科学基金青年科学基金项目(BK20130102);国家环境保护公益性行业科研专项(201209024)

Isolation and Identification of a Penicillin-Degrading Strain during Composting of Penicillin Bacteria Residue

  • 摘要: 为了研究开发青霉素发酵菌渣堆肥资源化与无害化技术,采用传统的富集、分离、纯化等微生物学方法,在青霉素菌渣与猪粪混合堆肥过程中筛选出一株青霉素钠高效降解菌——PC-2,并对其进行形态表征和基于16S rRNA基因序列的微生物种属鉴定. 结果表明:菌株PC-2属螯合球菌属(Chelatococcus sp.),其能够利用青霉素钠为唯一碳源生长,但外加碳、氮源可显著提高菌株PC-2对青霉素钠的降解效率. 当葡萄糖为碳源、蛋白胨为氮源、菌株PC-2接种量为14%、pH为6~8时,菌株PC-2在37 ℃下振荡培养6 h,对初始ρ(青霉素钠)为400 mg/L的青霉素钠的降解率可达98%以上. 自堆肥过程中获取高效青霉素钠降解菌PC-2,预示着其在菌渣堆肥过程中的应用潜力,也有助于深入开展青霉素制药菌渣的安全有效与无害化处理处置方法的研究.

     

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出版历程
  • 收稿日期:  2015-09-04
  • 修回日期:  2015-11-06
  • 刊出日期:  2016-02-25

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