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含四环素沼肥对蔬菜品质及抗性基因的影响

李盼盼 宋雯 谷洁 张凯煜 张社奇 王小娟 高华

李盼盼, 宋雯, 谷洁, 张凯煜, 张社奇, 王小娟, 高华. 含四环素沼肥对蔬菜品质及抗性基因的影响[J]. 环境科学研究, 2016, 29(6): 907-915.
引用本文: 李盼盼, 宋雯, 谷洁, 张凯煜, 张社奇, 王小娟, 高华. 含四环素沼肥对蔬菜品质及抗性基因的影响[J]. 环境科学研究, 2016, 29(6): 907-915.
LI Panpan, SONG Wen, GU Jie, ZHANG Kaiyu, ZHANG Sheqi, WANG Xiaojuan, GAO Hua. Effects of Biogas Fertilizers Containing Tetracycline on Vegetable Quality and Resistance Genes[J]. Research of Environmental Sciences, 2016, 29(6): 907-915.
Citation: LI Panpan, SONG Wen, GU Jie, ZHANG Kaiyu, ZHANG Sheqi, WANG Xiaojuan, GAO Hua. Effects of Biogas Fertilizers Containing Tetracycline on Vegetable Quality and Resistance Genes[J]. Research of Environmental Sciences, 2016, 29(6): 907-915.

含四环素沼肥对蔬菜品质及抗性基因的影响

基金项目: 国家自然科学基金项目(41171203);引进国际先进农业科学技术计划(948计划)项目(2015-Z37);陕西省科技统筹创新工程计划项目(2015KTCL03-19)

Effects of Biogas Fertilizers Containing Tetracycline on Vegetable Quality and Resistance Genes

  • 摘要: 为探讨含四环素沼肥的生态毒性,采用盆栽试验研究了含四环素沼肥对小白菜品质、土壤微生物群落代谢功能多样性及抗性基因的影响. 结果表明:与CK相比,添加高浓度(15 mg/kg)四环素沼肥处理下,小白菜鲜质量和w(维生素C)分别降低了5.7%和16.7%,w(硝酸盐)增加了34.3%. Biolog微平板分析显示,添加高浓度四环素的沼肥显著提高了土壤微生物群落碳源利用多样性和物种丰富度. 实时荧光定量PCR分析发现,在小白菜幼苗期,添加高浓度四环素的沼肥处理下,土壤中四环素类抗性基因相对丰度比CK高出0.69~4.03倍. 无论沼肥中是否添加四环素,施肥后小白菜内生菌中均存在抗性基因,并且小白菜内生菌抗性基因相对丰度均以CK最高,比其他处理高出0.55~325.72倍. 研究显示,添加四环素的沼肥改变了小白菜品质和产量以及土壤微生物群落碳源利用多样性和物种丰富度,增加了土壤中四环素类抗性基因丰度,降低了小白菜内生菌的抗性基因水平.

     

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出版历程
  • 收稿日期:  2015-11-24
  • 修回日期:  2016-03-10
  • 刊出日期:  2016-06-25

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