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双酚A对豚鼠的免疫毒性

金明兰 李英燮 赵玉鑫 徐莹莹 薛洪海 王颖 金宁一

金明兰, 李英燮, 赵玉鑫, 徐莹莹, 薛洪海, 王颖, 金宁一. 双酚A对豚鼠的免疫毒性[J]. 环境科学研究, 2016, 29(9): 1311-1317.
引用本文: 金明兰, 李英燮, 赵玉鑫, 徐莹莹, 薛洪海, 王颖, 金宁一. 双酚A对豚鼠的免疫毒性[J]. 环境科学研究, 2016, 29(9): 1311-1317.
JIN Minglan, LI Yingxie, ZHAO Yuxin, XU Yingying, XUE Honghai, WANG Ying, JIN Ningyi. Immunotoxicity Evaluation of Bisphenol A on Guinea Pigs[J]. Research of Environmental Sciences, 2016, 29(9): 1311-1317.
Citation: JIN Minglan, LI Yingxie, ZHAO Yuxin, XU Yingying, XUE Honghai, WANG Ying, JIN Ningyi. Immunotoxicity Evaluation of Bisphenol A on Guinea Pigs[J]. Research of Environmental Sciences, 2016, 29(9): 1311-1317.

双酚A对豚鼠的免疫毒性

基金项目: 吉林省教育厅“十三五”科学研究规划项目(20150168);国家自然科学基金项目(51208226)

Immunotoxicity Evaluation of Bisphenol A on Guinea Pigs

  • 摘要: 为了研究BPA(bisphenol A,双酚A)对豚鼠的免疫毒性,经口灌服,以不同剂量(500、1 000 mg/kg)染毒14 d后接种O型口蹄疫疫苗,通过分析脏器系数、淋巴细胞增殖能力、NK细胞(natural killer cell,自然杀伤细胞)活性、特异性抗体水平以及细胞免疫水平,评价BPA染毒剂量对豚鼠体液免疫和细胞免疫的影响.结果表明:与阴性对照组相比,BPA染毒后豚鼠体质量增加,心脏、肝脏、肾脏、脑、脾脏和卵巢体质量也增加,相应脏器系数升高;而胸腺、睾丸和附睾萎缩,相应脏器系数降低.NK细胞数量虽有增加,但变化不显著;BPA染毒引起NK细胞对靶细胞的杀伤能力显著下降,并且随着BPA染毒剂量的增加,其对靶细胞的杀伤能力显著下降;淋巴细胞刺激指数显著升高,并与染毒剂量呈正相关.BPA对豚鼠的特异性抗体水平有一定抑制作用,并且随着染毒剂量的增加该抑制作用愈发显著.IL-2(白介素2,interleukin-2)和IFN-γ(干扰素-γ,interferon)水平低于阴性对照组,而IL-4(白介素4,interleukin-4)水平高于阴性对照组;随着染毒剂量的增加,淋巴细胞增殖能力、IL-2和IFN-γ水平显著降低,而IL-4水平显著升高.研究显示,BPA会损伤豚鼠的免疫器官,抑制机体产生体液免疫和细胞免疫,导致免疫功能紊乱.

     

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出版历程
  • 收稿日期:  2015-12-20
  • 修回日期:  2016-05-14
  • 刊出日期:  2016-09-25

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