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不同pH下糖蜜废水的厌氧产酸发酵类型及微生物群落结构解析

昌盛 刘枫

昌盛, 刘枫. 不同pH下糖蜜废水的厌氧产酸发酵类型及微生物群落结构解析[J]. 环境科学研究, 2016, 29(9): 1370-1377.
引用本文: 昌盛, 刘枫. 不同pH下糖蜜废水的厌氧产酸发酵类型及微生物群落结构解析[J]. 环境科学研究, 2016, 29(9): 1370-1377.
CHANG Sheng, LIU Feng. Analysis of the Dynamics of the Microbial Community and Changing of Acidic Fermentation Type of an Anaerobic Reactor Treating Refinery Molasses Wastewater under Different Controlled pH Levels[J]. Research of Environmental Sciences, 2016, 29(9): 1370-1377.
Citation: CHANG Sheng, LIU Feng. Analysis of the Dynamics of the Microbial Community and Changing of Acidic Fermentation Type of an Anaerobic Reactor Treating Refinery Molasses Wastewater under Different Controlled pH Levels[J]. Research of Environmental Sciences, 2016, 29(9): 1370-1377.

不同pH下糖蜜废水的厌氧产酸发酵类型及微生物群落结构解析

基金项目: [ZK(]国家自然科学基金项目(51508539);国家水体污染控制与治理科技重大专项(2014ZX07405-001)[ZK)]

Analysis of the Dynamics of the Microbial Community and Changing of Acidic Fermentation Type of an Anaerobic Reactor Treating Refinery Molasses Wastewater under Different Controlled pH Levels

  • 摘要: 为寻求厌氧产酸发酵反应器的适宜控制参数和微生物学机制,以ACR(厌氧接触式发酵制氢反应器)为试验平台,通过分阶段调控反应器的pH,考察不同pH下ACR系统的产酸发酵类型和产氢性能,并采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术对微生物群落结构进行了解析.以糖蜜废水为基质,以城市污水厂剩余污泥为种泥,在污泥接种量(以MLVSS计)为3.58 g/L、进水ρ(CODCr)为5 000 mg/L、HRT为12 h条件下,分别考察了系统在pH为6.0~6.5、5.5~6.0、5.0~5.5、4.5~5.0条件下的运行特性.结果表明,在pH为4.5~5.0时,系统中以Ethanoligenens harbinense YUAN-3为代表的产氢菌群占优,呈现典型的乙醇型发酵特征,活性污泥表现出的产氢性能最佳,其氢气转化率和污泥的比产氢速率分别为1.9 mol/mol和7.8 mmol/(g·d).在pH为5.5~6.5、5.0~5.5的条件下,Clostridium tyrobutyricum strain A1-3、Propionibacterium sp.B2M2分别成为优势菌群,系统分别呈现出丁酸型发酵、混合酸发酵类型.研究显示,随着pH的改变,系统内的产酸发酵菌群随之发生更迭,在不同pH控制水平下形成不同的顶级微生物群落结构,进而使得系统呈现出不同发酵类型和产氢性能.

     

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出版历程
  • 收稿日期:  2016-01-26
  • 修回日期:  2016-04-28
  • 刊出日期:  2016-09-25

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