留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

碳酸盐矿化菌固结Pb2+的生物矿化

成艳 赵兴青

成艳, 赵兴青. 碳酸盐矿化菌固结Pb2+的生物矿化[J]. 环境科学研究, 2016, 29(10): 1513-1520.
引用本文: 成艳, 赵兴青. 碳酸盐矿化菌固结Pb2+的生物矿化[J]. 环境科学研究, 2016, 29(10): 1513-1520.
CHENG Yan, ZHAO Xingqing. Study on the Consolidation and Mineralization of Pb2+ by Carbonate-Mineralization Bacteria[J]. Research of Environmental Sciences, 2016, 29(10): 1513-1520.
Citation: CHENG Yan, ZHAO Xingqing. Study on the Consolidation and Mineralization of Pb2+ by Carbonate-Mineralization Bacteria[J]. Research of Environmental Sciences, 2016, 29(10): 1513-1520.

碳酸盐矿化菌固结Pb2+的生物矿化

基金项目: 国家自然科学基金项目(41302025,41541016)

Study on the Consolidation and Mineralization of Pb2+ by Carbonate-Mineralization Bacteria

  • 摘要: 为了研究碳酸盐矿化菌在修复重金属污染过程中的生物矿化作用及其在不同污染体系下对生物矿化的影响,以从南京栖霞山土壤中筛选出的一株耐受Pb的碳酸盐矿化菌株——Bacillus cereus Pb21为研究对象,选用葡萄糖作为底物,采用扫描电子显微镜(SEM)和X射线衍射(XRD)等分析测试手段对其矿化产物进行表征.结果表明:碳酸盐矿化菌可以利用2%葡萄糖底物诱导下的酶化作用将游离态Pb2+转变为稳定态PbCO3,从而达到修复环境中Pb2+污染的目的.不同污染体系下的生物矿化分析结果显示,随着pH的升高,矿化产物的平均粒径也在变大,由pH为3时的约24 μm增至pH为9时的99 μm.而且,当环境溶液为中性或碱性(pH为7、9)时,矿物生成的速度较快,结晶较好,Pb2+的固结率达到90%以上;而当pH较低(pH为3、5)时,晶体不易成核生长,Pb2+的固结率低于60%.ρ(Pb2+)对Pb2+固结率的影响差异较小,几乎都在90%以上,但是其对矿化产物结构形态的影响较大,随着ρ(Pb2+)的增加,矿化产物的形态越清晰,对微生物矿化固结重金属形成矿化产物也越有利.

     

  • [1] WANG X Y.Characteristic and environmental risk assessment of heavy metals in farmland soil of based on speciation analysis.Informatics and Management Science I,2013,204:213-220.
    [2] FAROUK S,MOSA A A,TAHA A A,et al.Protective effect of humic acid and chitosan on radish(Raphanus sativus,L.var.sativus) plants subjected to cadmium stress.Journal of Stress Physiology & Biochemistry,2011,7(2):99-116.
    [3] NEMECEK J,LHOTSKY O,TOMAS C.Nanoscale zero-valent iron application for in situ reduction of hexavalent chromium and its effects on indigenous microorganism populations.Science of the Total Environment,2014,485/486:739-747.
    [4] 罗辉,朱易春,冯秀娟.重金属污染土壤的生物修复技术研究进展.安徽农业科学,2015,43(5):224-227.LUO Hui,ZHU Yichun,FENG Xiujuan.Research progress of bioremediation on heavy-metal contaminated soil.Journal of Anhui Agricultural Sciences,2015,43(5):224-227.
    [5] YAO Zhitong,LI Jinhui,XIE Henghua,et al.Review on remediation technologies of soil contaminated by heavy metals.Procedia Environmental Sciences,2012,16:722-729.
    [6] 孙约兵,王永昕,李烨,等.Cd-Pb复合污染土壤钝化修复效率与生物标记物识别.环境科学研究,2015,28(6):951-958.SUN Yuebing,WANG Yongxin,LI Ye,et al.Effectiveness of immobilization remediation of Cd and Pb combined contaminated soil and biomarker identification.Research of Environmental Sciences,2015,28(6):951-958.
    [7] BEATH J M.Consider phytoremediation for waste site cleanup.Chemical Engineering Progress,2000,96(7):61-69.
    [8] BART V,JURGEN S.Earthworm biomass as additional information for risk assessment of heavy metal biomagnification:a case study for dredged sediment-derived soils and polluted floodplain soils.Environmental Pollution,2004,129(3):363-375.
    [9] MORGAN J A W,BENDING G D,WHITE P J.Biological costs and benefits to plant-microbe interactions in the rhizosphere.Journal of Experimental Botany,2005,56(417):1729-1739.
    [10] 赵庆龄,张乃弟,路文如.土壤重金属污染研究回顾与展望.Ⅱ:基于三大学科的研究热点与前沿分析.环境科学与技术,2010,33(7):102-106.ZHAO Qingling,ZHANG Naidi,LU Wenru.Research review and prospect on soil heavy metals pollution:Ⅱ.research focus and analysis based on three major disciplines.Environmental Science & Technology(China),2010,33(7):102-106.
    [11] LOWENSTAM H A,WEINER S.On biomineralization.New York:Oxford University Press,1989.
    [12] MANN S.Biomineralization:principles and concepts in bioinorganic materials chemistry.New York:Oxford University Press,2001.
    [13] GOVARTHANAN M,LEE K J,CHO M,et al.Significance of autochthonous Bacillus sp.KK1 on biomineralization of lead in mine tailings.Chemosphere,2013,90(8):2267-2272.
    [14] FATMA M H,HEMDAN R E,SHERIF M E,et al.Calcium carbonate precipitation induced by ureolytic bacteria Bacillus licheniformis.Ecological Engineering,2016,90:367-371.
    [15] GADD G M,FRY J C.Microbial control of pollution.Cambridge,UK:Cambridge University Press,1992:59-84.
    [16] WANG Lina,HUO Jichuan,LIU Shuxin,et al.A new route to the synthesis of barium carbonate crystals by the induction of Bacillus pasteurii.Chinese Journal of Structural Chemistry,2011,30(5):738-742.
    [17] 王瑞兴,钱春香,吴淼,等.微生物矿化固结土壤中重金属研究.功能材料,2007,38(9):1523-1526.WANG Ruixing,QIAN Chunxiang,WU Miao,et al.Study on heavy metals in soil mineralized by bacteria.Functional Materials,2007,38(9):1523-1526.
    [18] CHEN Zhi,PAN Xiaohong,CHEN Hui.Biomineralization of Pb(Ⅱ) into Pb-hydroxyapatite induced by Bacillus cereus 12-2 isolated from lead-zinc mine tailings.Journal of Hazardous Materials,2016,301(15):531-537.
    [19] 刘家源.硫化物矿山环境中铅耐受性微生物的分离、鉴定以及系统发育分析.常州:常州大学,2013:11-22.
    [20] 谢家仪,董光军,刘振英.扫描电镜的微生物样品制备方法.电子显微学报,2005,24(4):440.
    [21] 周胜波,李庚飞,侯新凯.矿渣水泥浆体中结晶矿物的XRD分析.中国矿业,2008,17(12):88-93.ZHOU Shengbo,LI Gengfei,HOU Xinkai.Analysis on crystal minerals of the slag cement paste by XRD.China Mining Magazine,2008,17(12):88-93.
    [22] MASON J A,GREENE R S B,JOECKEL R M.Laser diffraction analysis of the disintegration of aeolian sedimentary aggregates in water.Catena,2011,87(1):107-118.
    [23] LOWENSTAM H A.Minerals formed by organisms.Science,1981,211(4487):1126-1131.
    [24] WEINER B S,DOVE P M.An overview of biomineralization processes and the problem of the vital effect.Review in Mineralogy & Geochemistry,2003,54(1):1-29.
    [25] ACHAL V,PAN Xiangliang,ZHANG Daoyong.Bioremediation of strontium(Sr) contaminated aquifer quartz sand based on carbonate precipitation induced by Sr resistant Halomonas sp..Chemosphere,2012,89(6):764-768.
    [26] LIAN Bin,HU Qiaona,CHEN Jun,et al.Carbonate biomineralization induced by soil bacterium Bacillusmegaterium.Geochimica et Cosmochimica Acta,2006,70:5522-5535.
    [27] 黄婷,刘明学,董发勤,等.不同微生物诱导钙锶生物矿化的比较研究.高校地质学报,2015,21(4):584-593.HUANG Ting,LIU Mingxue,DONG Faqin,et al.The comparative studies on Ca2+/Sr2+ biomineralization induced by different microbes.Geological Journal of China Universities,2015,21(4):584-593.
    [28] 王明明,钱春香.磷酸盐矿化菌矿化重金属离子Zn2+的研究.功能材料,2013,44(3):393-395.WANG Mingming,QIAN Chunxiang.Study on heavy metal ion Zn2+ mineralized by phosphate ore implication of bacteria.Functional Materials,2013,44(3):393-395.
    [29] 陆兆文,钱春香,许燕波,等.不同污染条件下微生物矿化固结Zn2+的作用及机理.东南大学学报(自然科学版),2013,43(2):364-367.LU Zhaowen,QIAN Chunxiang,XU Yanbo,et al.Function and mechanism of microbial mineralization consolidation of Zn2+ under different pollution conditions.Journal of Southeast University(Natural Science Edition),2013,43(2):364-367.
    [30] 黄江波.多糖/蛋白质调控纳米铁矿物生物矿化作用机理研究.武汉:武汉理工大学,2006:21-22.
    [31] 成亮,钱春香,王瑞兴,等.碳酸盐矿化菌株A固结土壤Cd2+的生物矿化过程.硅酸盐学报,2008,36(S1):216-222.CHENG Liang,QIAN Chunxiang,WANG Ruixing,et al.Bioremediation process of Cd2+ removal from soil by bacteria a biomineralization.Journal of the Chinese Ceramic Society,2008,36(S1):216-222.
    [32] 竹文坤,牟涛,段涛,等.碳酸盐矿化菌对模拟放射性Sr2+的成矿作用.环境科学研究,2015,28(1):158-159.ZHU Wenkun,MOU Tao,DUAN Tao,et al.Metallogenesis of simulated radioactive Sr2+ by carbonate-mineralization bacteria.Research of Environmental Sciences,2015,28(1):158-159.
  • 加载中
计量
  • 文章访问数:  1541
  • HTML全文浏览量:  17
  • PDF下载量:  1309
  • 被引次数: 0
出版历程
  • 收稿日期:  2016-03-25
  • 修回日期:  2016-07-04
  • 刊出日期:  2016-10-25

目录

    /

    返回文章
    返回