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SO2污染激活AT1R改变运动大鼠心肌胶原纤维形态学并导致心功能降低

陈平 胡琰茹 刘晓莉

陈平, 胡琰茹, 刘晓莉. SO2污染激活AT1R改变运动大鼠心肌胶原纤维形态学并导致心功能降低[J]. 环境科学研究, 2017, 30(11): 1739-1745. doi: 10.13198/j.issn.1001-6929.2017.02.99
引用本文: 陈平, 胡琰茹, 刘晓莉. SO2污染激活AT1R改变运动大鼠心肌胶原纤维形态学并导致心功能降低[J]. 环境科学研究, 2017, 30(11): 1739-1745. doi: 10.13198/j.issn.1001-6929.2017.02.99
CHEN Ping, HU Yanru, LIU Xiaoli. SO2 Pollution Activation of AT1R Altering the Morphology of Myocardial Collagen Fibers of Exercised Rats and Leading to Decreased Cardiac Function[J]. Research of Environmental Sciences, 2017, 30(11): 1739-1745. doi: 10.13198/j.issn.1001-6929.2017.02.99
Citation: CHEN Ping, HU Yanru, LIU Xiaoli. SO2 Pollution Activation of AT1R Altering the Morphology of Myocardial Collagen Fibers of Exercised Rats and Leading to Decreased Cardiac Function[J]. Research of Environmental Sciences, 2017, 30(11): 1739-1745. doi: 10.13198/j.issn.1001-6929.2017.02.99

SO2污染激活AT1R改变运动大鼠心肌胶原纤维形态学并导致心功能降低

doi: 10.13198/j.issn.1001-6929.2017.02.99
基金项目: 

国家自然科学基金项目 31571221

详细信息
    作者简介:

    陈平(1983-), 男, 湖北郧县人, 315339318@qq.com

    通讯作者:

    刘晓莉(1958-), 女, 山西太原人, 教授, 博士, 博导, 主要从事体育保健与运动营养研究, xiaolil@bnu.edu.cn

  • 中图分类号: X503;G804.34

SO2 Pollution Activation of AT1R Altering the Morphology of Myocardial Collagen Fibers of Exercised Rats and Leading to Decreased Cardiac Function

Funds: 

 31571221

  • 摘要: 为了观察SO2污染环境下运动对大鼠心功能的影响,从心脏局部肾素-血管紧张素系统(renin-angiotensin system,RAS)核心成员——血管紧张素Ⅱ(angiotensin Ⅱ,AngⅡ)Ⅰ型受体(AT1R)介导的心肌胶原纤维形态结构重塑的角度出发,应用心脏插管技术观察大鼠的心脏功能;采用放射免疫技术(ELISA)、免疫组织化学和胃酶酸解法等方法对心肌局部ρ(AngⅡ)、AT1R蛋白表达水平、w(HYP)(HYP为羟脯氨酸)及胶原容积分数进行检测.结果表明:①单纯运动组(EG)大鼠主动脉收缩压、左室内压峰值、±dp/dtmax(左室内压最大上升速率/下降速率)显著升高(P < 0.01),舒张压、AT1R蛋白表达显著降低(P < 0.01),w(HYP)、w(CC)(心肌胶原浓度)、PVCA(血管周围胶原面积)、CVF(心肌胶原容积分数)及ρ(AngⅡ)有升高趋势(P>0.01);②单纯SO2污染组(SRG)大鼠左室末期舒张压显著升高(P < 0.01),左室内压±dp/dtmax显著降低(P < 0.01);w(HYP)、w(CC)、PVCA、CVF、ρ(AngⅡ)及AT1R蛋白表达水平均显著升高(P < 0.01);③SO2污染+运动组(SEG)大鼠左室末期舒张压显著升高(P < 0.01),主动脉收缩压、左室内压峰值、左室内压±dp/dtmax显著降低(P < 0.01),w(HYP)、w(CC)、PVCA、CVF、ρ(AngⅡ)及AT1R蛋白表达水平均显著升高(P < 0.01),并且较SRG大鼠升高更显著(P < 0.01).研究显示,SO2污染导致运动大鼠心肌胶原纤维形态结构发生异常重塑,最终使大鼠的心功能产生显著的负性变力性效应,其机制可能与心脏局部RAS系统的激活有关.

     

  • 图  1  大鼠心肌组织胶原纤维和小动脉周围胶原形态学变化

    注:黄色为心肌组织,红色为胶原纤维.

    Figure  1.  The change of myocardial collagen fiber morphology and around the small artery collagen fiber morphology

    图  2  大鼠心肌w(HYP)、w(CC)、CVF%和PVCA%的变化

    注:与RG相比,*表示P < 0.05,**表示P < 0.01;与EG相比,#表示P < 0.05,##表示P < 0.01;与SRG相比,&表示P < 0.05,&&表示P < 0.01.下同.

    Figure  2.  The change of hydroxyproline in myocardium, myocardial collagen content, myocardial collagen volume fraction and around the blood vessels collagen volume fraction

    图  3  大鼠心肌ρ(Ang Ⅱ)的变化

    Figure  3.  The change of Ang II content in myocardium

    图  4  心肌AT1R蛋白表达水平的变化

    Figure  4.  The change of expression of AT1R protein in myocardium

    表  1  各组大鼠BW、HW、HBI、LVM、LVMI的变化

    Table  1.   The change of BW, HW, HBI, LVM and LVMI in each group of rats

    组别 BW/g HW/g HBI/(mg/g) LVM/g LVMI/(mg/g)
    RG 354.0±18.50 1.16±0.09 0.327±0.018 0.72±0.1 0.203±0.04
    EG 312.50±15.66** 1.41±0.10** 0.452±0.024** 0.80±0.07* 0.256±0.02**
    SRG 307.00±13.03** 1.20±0.22## 0.391±0.079*# 0.74±0.03# 0.241±0.02*
    SEG 289.00±19.88**##&& 1.52±0.19**#&& 0.526±0.006**##&& 0.86±0.04**#&& 0.298±0.03**##&&
      注:数据为x±SD,n=6.与RG相比,*表示P < 0.05,**表示P < 0.01;与EG相比,#表示P < 0.05,##表示P < 0.01;与SRG相比,&表示P < 0.05,&&表示P < 0.01.下同.
    下载: 导出CSV

    表  2  大鼠左室功能的变化

    Table  2.   The change of left ventricular function in rats

    指标 RG EG SRG SEG
    SBP/hPa 141.58±4.65 159.73±5.60** 143.71±5.14 129.38±3.93**##&&
    DBP/hPa 107.34±6.12 85.57±4.33** 112.20±6.9## 113.70±3.04*##
    LVSP/hPa 152.72±3.07 173.57±4.13** 153.07±3.97## 134.50±4.36**##&&
    LVEDP/hPa 3.59±0.95 3.11±0.69 5.27±1.21*## 6.63±1.43**##&
    (+dp/dtmax)/(hPa/s) 4 427.45±186.68 6 289.43±323.60** 3 967.17±340.28**# 3 297.40±267.47**##&&
    (-dp/dtmax)/(hPa/s) -4 565.43±212.80 -6 555.69±768.90** -3 505.67±135.60*## -2 992.34±345.61**##&
    下载: 导出CSV
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  • 收稿日期:  2017-03-22
  • 修回日期:  2017-07-07
  • 刊出日期:  2017-11-25

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