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甲基丙烯酸树脂吸附微生物降解与演化石油烃特性

雷忠春 陈丽华 孙万虹 雒晓芳 张文博

雷忠春, 陈丽华, 孙万虹, 雒晓芳, 张文博. 甲基丙烯酸树脂吸附微生物降解与演化石油烃特性[J]. 环境科学研究, 2019, 32(1): 142-149. doi: 10.13198/j.issn.1001-6929.2018.09.12
引用本文: 雷忠春, 陈丽华, 孙万虹, 雒晓芳, 张文博. 甲基丙烯酸树脂吸附微生物降解与演化石油烃特性[J]. 环境科学研究, 2019, 32(1): 142-149. doi: 10.13198/j.issn.1001-6929.2018.09.12
LEI Zhongchun, CHEN Lihua, SUN Wanhong, LUO Xiaofang, ZHANG Wenbo. Biological Degradation and Transformation Characteristics of Total Petroleum Hydrocarbon by Oil-Degradation Bacteria Adsorbed on the Resin Made by Methylacrylic Acid Butyi Ester[J]. Research of Environmental Sciences, 2019, 32(1): 142-149. doi: 10.13198/j.issn.1001-6929.2018.09.12
Citation: LEI Zhongchun, CHEN Lihua, SUN Wanhong, LUO Xiaofang, ZHANG Wenbo. Biological Degradation and Transformation Characteristics of Total Petroleum Hydrocarbon by Oil-Degradation Bacteria Adsorbed on the Resin Made by Methylacrylic Acid Butyi Ester[J]. Research of Environmental Sciences, 2019, 32(1): 142-149. doi: 10.13198/j.issn.1001-6929.2018.09.12

甲基丙烯酸树脂吸附微生物降解与演化石油烃特性

doi: 10.13198/j.issn.1001-6929.2018.09.12
基金项目: 

国家民委中青年英才人才项目 2016-CNPC-57

中国石油集团川庆钻探工程有限公司技术开发项目 Cqzt-cqjx-2017-zcj-032

中央高校基本科研业务基金项目 31920170169

详细信息
    作者简介:

    雷忠春(1994-), 男(畲族), 福建福州人, 907152239@qq.com

    通讯作者:

    陈丽华(1972-), 女, 宁夏中卫人, 教授, 博士, 主要从事土壤有机物污染修复机理与技术研究, clh@xbmu.edu.cn

  • 中图分类号: X53

Biological Degradation and Transformation Characteristics of Total Petroleum Hydrocarbon by Oil-Degradation Bacteria Adsorbed on the Resin Made by Methylacrylic Acid Butyi Ester

Funds: 

National Civil Affairs Commission Young and Middle-AGED TAlents Project, China 2016-CNPC-57

CNPC Chuanqing Drilling Engineering Co., Ltd. Technical Development Project, China Cqzt-cqjx-2017-zcj-032

Basic Scientific Research Fund Project of Central University, China 31920170169

  • 摘要: 为了提高石油污染土壤的生物降解效率,以甲基丙烯酸丁酯制备的树脂为载体吸附石油降解菌进行生物降解,利用GC-MS“指纹图谱”对TPH(total petroleum hydrocarbon,总石油烃)的降解程度及生物演化特性进行分析.结果表明:树脂吸附菌剂后TPH的半衰期为24.23 d,比单纯菌剂降解缩短7.28 d;其中正构烷烃的降解性能表明,高碳数高达92.03%,中、低碳数皆超过75%,高于TPH的56.67%,较好地解释了TPH降解率比单纯菌剂提高的内在原因;在演化特征方面,正构烷烃的生物演化参数——w(∑C21)/w(∑C22+)(C21-、C22+分别指C14~C21和C22~C39)、姥植比〔w(pr)/w(ph)〕和OEP(奇/偶碳数质量比)分别为0.643 3、0.486 5和1.111 9,均高于单纯菌剂演化程度.研究显示,树脂吸附菌剂后对较难降解的偶数碳正构烷烃和类异戊二烯烃类物质的氧化还原能力增强,从生物演化的角度有力地佐证了降解的优势所在.

     

  • 图  1  合成的树脂成品

    Figure  1.  Synthetic resin product

    图  2  树脂的红外光谱图

    Figure  2.  FT-IR spectra of resin

    图  3  树脂的晶体衍射图

    Figure  3.  XRD patterns of resin

    图  4  1 g树脂在不同时间下的吸油量

    Figure  4.  Oil absorption of 1g resin at different time

    图  5  TPH的降解动力学特征

    Figure  5.  The dynamic sorption characteristics of TPH

    图  6  饱和烃的GC/MS总离子流图

    Figure  6.  Total ion GC/MS chromatogram of saturated hydrocarbon

    图  7  降解后正构烷烃、类异戊二烯烷烃GC-MS离子流图

    峰号对应物质:1—正十四烷;2—正十五烷;3—正十六烷;4—正十七烷;5—姥鲛烷;6—正十八烷;7—植烷;8—正十九烷;9—正二十烷;10—44-氘代二十一烷;11—正二十一烷;12—正二十二烷;13—正二十三烷;14—正二十四烷;15—正二十五烷;16—正二十六烷;17—正二十七烷;18—正二十八烷;19—正二十九烷;20—正三十烷;21—正三十一烷;22—正三十二烷;23—正三十三烷;24—正三十四烷;25—正三十五烷.

    Figure  7.  Total ion GC/MS chromatogram of of n-alkanes, isoprene alkanes after degradation

    图  8  不同碳数正构烷烃降解49 d后的残留量

    Figure  8.  Residue of n-alkane with different carbon numbers after 49 d degradation

    表  1  降解试验设计

    Table  1.   Experimental design of degradation

    处理组 土样质量/
    g
    标准油质量/
    g
    树脂质量/
    g
    菌剂体积/
    mL
    空白组 500 15 0 0
    对照组 500 15 0 20
    试验组 500 15 25 20
    下载: 导出CSV

    表  2  TPH在不同条件下降解动力学参数

    Table  2.   TPH drops the dynamics parameters in different condition

    处理组 k 半衰期/d
    空白组 0.009 8 70.72
    对照组 0.022 0 31.51
    试验组 0.028 6 24.23
    下载: 导出CSV

    表  3  不同碳数正构烷烃降解49 d后的残留量分类

    Table  3.   Classification of residue of n-alkane with different carbon numbers after 49 d degradation

    mg/L
    组别 C14~C20 C21~C30 C31~C39
    空白组 0.219 1 0.437 0 0.121 3
    对照组 0.097 7 0.373 5 0.101 0
    试验组 0.047 2 0.109 2 0.009 7
    下载: 导出CSV

    表  4  正构烷烃生物演化参数值

    Table  4.   N-Alkanes biological evolution parameter

    组别 主峰碳 姥植比 OEP w(∑C21-)/w(∑C22+)
    空白组 C28 0.432 0 0.723 7 0.282 6
    对照组 C25 0.463 3 0.992 1 0.577 1
    试验组 C23 0.486 5 1.111 9 0.643 3
    下载: 导出CSV
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  • 收稿日期:  2018-03-31
  • 修回日期:  2018-09-06
  • 刊出日期:  2019-01-25

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