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EBPR工艺污泥中聚磷菌多样性与除磷潜力评价方法

郑少奎 罗焇湝

郑少奎, 罗焇湝. EBPR工艺污泥中聚磷菌多样性与除磷潜力评价方法[J]. 环境科学研究, 2022, 35(10): 2338-2347. doi: 10.13198/j.issn.1001-6929.2022.06.04
引用本文: 郑少奎, 罗焇湝. EBPR工艺污泥中聚磷菌多样性与除磷潜力评价方法[J]. 环境科学研究, 2022, 35(10): 2338-2347. doi: 10.13198/j.issn.1001-6929.2022.06.04
ZHENG Shaokui, LUO Xiaojie. PAO Phylogenetic Diversity in Activated Sludge and Its Contribution to Phosphorus Removal by EBPR Process[J]. Research of Environmental Sciences, 2022, 35(10): 2338-2347. doi: 10.13198/j.issn.1001-6929.2022.06.04
Citation: ZHENG Shaokui, LUO Xiaojie. PAO Phylogenetic Diversity in Activated Sludge and Its Contribution to Phosphorus Removal by EBPR Process[J]. Research of Environmental Sciences, 2022, 35(10): 2338-2347. doi: 10.13198/j.issn.1001-6929.2022.06.04

EBPR工艺污泥中聚磷菌多样性与除磷潜力评价方法

doi: 10.13198/j.issn.1001-6929.2022.06.04
基金项目: 国家自然科学基金项目(No.51878050, 22176015)
详细信息
    作者简介:

    郑少奎(1973-),男,湖北咸宁人,教授,博士,博导,主要从事水和废水处理技术研究,zsk@bnu.edu.cn

  • 中图分类号: X703.1

PAO Phylogenetic Diversity in Activated Sludge and Its Contribution to Phosphorus Removal by EBPR Process

Funds: National Natural Science Foundation of China (No.51878050, 22176015)
  • 摘要: 自1970年代研究者发现聚磷菌(polyphosphate accumulating organism, PAO)并提出经典的强化生物除磷(enhanced biological phosphorus removal, EBPR)工艺“厌氧释磷-好氧摄磷”机理以来,随着EBPR工艺中PAO新菌株的不断发现和生理生化特征研究的不断深入,研究者们对EBPR机理的认识一直在不断更新. 及时总结近40年来EBPR机理的研究进展,基于活性污泥中PAO菌株信息全面归纳PAO多样性特征,以此为依据客观评价目前活性污泥中PAO除磷潜力评价方法的不足并展望未来重点研究方向,对于推动EBPR工艺优化升级将具有非常重要的理论与实际意义. 本文全面调研了1980—2021年国际期刊相关文献,发现传统EBPR机理中厌氧内碳源合成、厌氧释磷意义等受到了较多质疑,反硝化聚磷新机理已获得广泛认同;活性污泥中PAO具有异常丰富的多样性,包含Acinetobacter(29%)、Pseudomonas(15%)、Tetrasphaera(4%)、Alcaligenes(4%)等42个菌属,部分PAO具有反硝化聚磷和异养硝化能力. 在目前主流的活性污泥PAO除磷潜力评价方法中,荧光原位杂交和定量PCR技术只以AccumulibacterAcinetobacter属PAO为检测对象,高通量测序和变性梯度凝胶电泳技术基于片面的PAO多样性信息作为分析依据,在此基础上PAO丰度所反映的PAO除磷潜力的准确性尚存在疑问,未来需要加强面向活性污泥PAO多样性的探针或特异性引物的研发. 与传统方法相比,EDTA法胞内多聚磷酸盐颗粒含量检测技术较为先进,但需要以PAO和非PAO菌株为参照深入阐明检测结果的边界.

     

  • 图  1  传统强化生物除磷工艺生化代谢途径[14]

    Figure  1.  Biochemical metabolic pathway of traditional EBPR process[14]

    图  2  反硝化除磷生化代谢途径[25]

    Figure  2.  Biochemical metabolic pathway of denitrifying phosphorus removal process[25]

    图  3  EBPR工艺中分离得到的PAO菌株

    Figure  3.  PAO isolates from EBPR process

    表  1  EBPR工艺活性污泥PAO丰度检测常用探针及序列

    Table  1.   Common probes and sequences labeling PAO in EBPR process

    探针类型探针序列(5′→3′)靶向微生物数据来源
    PAO462CCGTCATCTACWCAGGGTATTAACCandidatus accumulibacter文献[10]
    PAO651CCCTCTGCCAAACTCCAGCandidatus accumulibacter文献[10]
    PAO846GTTAGCTACGGCACTAAAAGGCandidatus accumulibacter文献[10]
    PAOMIXPAO462、PAO651、PAO846等量混合物Candidatus accumulibacter文献[31-34]
    下载: 导出CSV

    表  2  活性污泥PAO的ppk1基因常用引物及序列

    Table  2.   Common amplification primer pairs and sequences targeting ppk1 gene

    目的基因引物序列(5ʹ~3ʹ)扩增长度/bp数据来源
    Acc1)-ppk1Acc-ppk1-254fTCACCACCGACGGCAAGAC1 123文献[53]
    Acc-ppk1-1376rACGA TCA TCAGCATCTTGGC
    Acc-Ⅰ-ppk1Acc-ppk1-763fGACGAAGAAGCGGTCAAG408文献[54]
    Acc-ppk1-1170rAACGGTCATCTTGATGGC
    Acc-ⅡA-ppk1Acc-ppk1-893fAGTTCAATCTCACCGAGAGC105文献[54]
    Acc-ppk1-997rGGAACTTCAGGTCGTTGC
    Acc-ⅡB-ppk1Acc-ppk1-870fGATGACCCAGTTCCTGCTCG133文献[54]
    Acc-ppk1-1002rCGGCACGAACTTCAGATCG
    Acc-ⅡC-ppk1Acc-ppk1-254fTCACCACCGACGGCAAGAC207文献[54]
    Acc-ppk1-460rCCGGCATGACTTCGCGGAAG
    Acc-ⅡD-ppk1Acc-ppk1-375fGGGTATCCGTTTCCTCAAGCG148文献[54]
    Acc-ppk1-522rGAGGCTCTTGTTGAGTACACGC
    Most ppk1 homologsNLDECGTATGAATTTTCTTGGTATTTATTGTACTAATCTNGAYGARTTYT2)1 300文献[55]
    TGNYGTCGAGCAGTTTTTGCATGAWARTTNCCNGT2)
    Most ppk1 homologsppk1_FWAAYYTIGAYGARTTYTTYATGGT2)1 100文献[56]
    ppk1_RWTTIKYITSYTCRTCRAAICKIGC2)
    注:1)表示Accumulibacter;2)表示氨基酸序列.
    下载: 导出CSV
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  • 收稿日期:  2022-02-26
  • 修回日期:  2022-05-23

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