Abstract: Abstract: In recent years, the problem of antibiotic contamination in landfill leachates and the surrounding environment has received increasing attention. However, the information in this regard is relatively lacking in Hainan Province. In this paper, three typical landfills in Hainan Province were selected to investigate the contamination characteristics of 17 antibiotics, including sulfonamides (SAs), quinolones (QNs), lincosamides (LCMs) and tetracyclines (TCs), in leachates and surrounding groundwater, and to assess their ecological risks. The results showed that: (1) the average detection rate of LCMs in the three landfills was 70.83%, which was higher than that of other three antibiotics; among them, the average detection rates of lincomycin (LIN), clindamycin (CLIN) and sulfadiazine (SDZ) were relatively high, reaching 83.33%, 58.33% and 58.33%, respectively; (2) The types and total concentrations of antibiotics detected in leachate samples and surrounding groundwater samples from the same landfill were highly consistent. The relatively high concentrations of antibiotics detected in the leachate samples were LIN (1495.80~10648.80 ng/L), SDZ (1473.07~5910.07 ng/L) and Sulfisomidine (SIM, 518.67~1807.73 ng/L); the relatively high concentrations of antibiotics detected in the surrounding groundwater samples were SIM (ND ~58.10 ng/L), sulfaquinoxaline (SQX, ND ~17.77 ng/L), cinoxacin (CNX, ND ~27.71 ng/L) and CLIN (ND ~18.11 ng/L); (3) The ecological risk level of QNs was higher than the other three classes of antibiotics; the antibiotics with higher ecological risk levels were sulfapyridine (SPD), SDZ, sulfathiazole (STZ), ofloxacin (OFL), gatifloxacin (GTFX), CNX and LIN. These results can provide a reference for the control of antibiotic contamination in the environment around landfills.