Abstract: The vertical structures of ozone and meteorology in border area of Jiangsu, Anhui, Shandong and Henan in November 2021 were observed by using lidar. The vertical distribution characteristics of ozone were analyzed. The vertical distribution of ozone concentration, temperature and wind speed on clean days and ozone polluted days were compared. The relationship between ozone concentration distribution, temperature and wind speed at vertical height was explored. The results show that the surface ozone was characterized by single-peak daily variation. The ozone pollution occurred in the upper air from the 16th to the 18th. In the clean days, the ozone concentration below 1.5 km increased first and then decreased with altitude. The ozone distribution had double layers on the 16th and 17th of the polluted days; The ozone concentration decreased with the rise of altitude below 1.5 km on the 18th. At the vertical altitude, the temperature of the ozone polluted days was higher than that of the clean days, and the wind speed was lower than that of the clean days, that is, the high temperature and low wind were conducive to the existence of high concentration of ozone. The inversion layer on the 16th day played an important role in the accumulation of ozone concentration. Downdraft was also one of the important causes of ozone accumulation in the upper air on polluted days. The analysis of potential ozone sources showed that, except for local ozone generation, the ozone mainly came from Lu 'an City and the northern part of Jiangsu Province. By comparing the backward trajectories of air flows on clean days and polluted days, it can be seen that the upper ozone pollution on polluted days was mainly affected by the southern and eastern air flows.