土壤中三价锑的老化对秀丽隐杆线虫毒性的影响
Effects of Trivalent Antimony Aging on the Toxicity of Soil-dwelling Caenorhabditis elegans
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摘要: 为明确老化过程对土壤外源Sb(III)毒性的影响,结合土壤中Sb的化学分析和生物毒性测试,以模式生物秀丽隐杆线虫(Caenorhabditis elegans)为测试生物,研究经过7和56 d老化的2种Sb(III)处理土壤(安徽黄棕壤和新疆灰漠土)中不同价态Sb浓度、可提取态Sb浓度以及对线虫生长、生育、繁殖毒性的变化。经过56 d老化的安徽黄棕壤和新疆灰漠土中Sb(V)占比相较7 d老化出现显著提高,新疆灰漠土中Sb(III)相较安徽黄棕壤能更快的转化为毒性较低的Sb(V),可提取态Sb的提取比例相较7 d的老化分别下降了27.0%和32.5%。经过56 d老化的Sb(III)处理安徽黄棕壤和新疆灰漠土对线虫生长毒性的EC50(50%效应浓度)值相较7 d老化分别提升1.7和2.3倍;对线虫生育毒性的EC50值分别提升1.1和2.4倍;对线虫繁殖毒性的EC50值分别提升1.2和1.9倍。基于EC50值计算的新疆灰漠土中外源Sb(III)的老化因子高于安徽黄棕壤,与新疆灰漠土中Sb(III)较高的氧化效率和可提取态Sb下降比例具有一致性,pH、有机质和铁锰铝氧化物含量为2种土壤外源Sb(III)氧化效率和可提取态Sb浓度的差异提供一定程度上的解释。Abstract: To determine the effect of aging process on the toxicity of exogenous Sb(III) in soil, combining with chemical analysis and toxicity test, the changes of oxidation state, extracted concentration of exogenous Sb(III) and its growth, fertility, and reproduction toxicity on model organism Caenorhabditis elegans after 7 and 56 d aging in Anhui yellow-brown soil and Xinjiang grey dessert soil. The proportion of Sb(V) in Anhui yellow-brown soil and Xinjiang grey desert soil aged for 56 d were significantly higher than that aged for 7 d. Sb(III) in Xinjiang grey desert soil can be oxidized to Sb(V) faster than that in Anhui yellow-brown soil. Compared with 7 d aging, the ratio of extracted Sb in Anhui yellow-brown soil and Xinjiang grey desert soil with 56 d aging decreased by 27.0% and 32.5%; respectively; the EC50 (50% effect concentration) of exogenous Sb(III) on the growth toxicity of C. elegans increased by 1.7 and 2.3 times, respectively; the EC50 of exogenous Sb(III) on the fertility toxicity of C. elegans increased by 1.1 and 2.4 times, respectively; the EC50 of exogenous Sb(III) on the reproduction toxicity of C. elegans increased by 1.2 and 1.9 times, respectively. The aging factor of exogenous Sb (III) in Xinjiang grey desert soil calculated based on EC50 value were higher than that in Anhui yellow-brown soil, which was consistent with the higher oxidation efficiency of Sb(III) and the decline proportion of extracted Sb in Xinjiang grey desert soil. pH, organic matter and iron, manganese, aluminum oxide could explain the difference of exogenous Sb(III) oxidation efficiency and extracted Sb concentration between Anhui yellow-brown soil and Xinjiang grey desert soil.
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Key words:
- antimony /
- Caenorhabditis elegans /
- toxicity /
- aging effects /
- soil
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