Toxicity of Soil Antimony to <i>Eisenia fetida</i> at Individual and Cellular Levels

CHENG Jiaqi; MA Hongrui; CHANG Jian; PAN Minghui; LI Qinlian; WANG Qian; HOU Hong

doi:10.13198/j.issn.1001-6929.2022.11.10

Volume 36 Issue 1

Jan. 2023

Turn off MathJax

Article Contents

Article Navigation > Research of Environmental Sciences > 2023 > 36(1): 63-71

CHENG Jiaqi, MA Hongrui, CHANG Jian, PAN Minghui, LI Qinlian, WANG Qian, HOU Hong. Toxicity of Soil Antimony to Eisenia fetida at Individual and Cellular Levels[J]. Research of Environmental Sciences, 2023, 36(1): 63-71. doi: 10.13198/j.issn.1001-6929.2022.11.10

Citation:

CHENG Jiaqi, MA Hongrui, CHANG Jian, PAN Minghui, LI Qinlian, WANG Qian, HOU Hong. Toxicity of Soil Antimony to Eisenia fetida at Individual and Cellular Levels[J]. Research of Environmental Sciences, 2023, 36(1): 63-71. doi: 10.13198/j.issn.1001-6929.2022.11.10

Citation:

PDF( 940 KB)

Toxicity of Soil Antimony to Eisenia fetida at Individual and Cellular Levels

doi: 10.13198/j.issn.1001-6929.2022.11.10

CHENG Jiaqi^{1, 2
,},
MA Hongrui²,
CHANG Jian^{1, 3},
PAN Minghui³,
LI Qinlian²,
WANG Qian^4
,,
HOU Hong^{1
,
,}

1.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
2.
Shaanxi University of Science and Technology, Xi'an 710021, China
3.
North China University of Science and Technology, Tangshan 063000, China
4.
Technical Centre for Soil, Agriculture and Rural Ecology and Environment, Ministry of Ecology and Environment, Beijing 100012, China

Funds: National Key Research and Development Program of China (No.2020YFC1807703, 2019YFC1804603)

Received Date: 2022-06-25
Rev Recd Date: 2022-10-26

Abstract

Abstract

With the exploitation of antimony (Sb) ore, the exposure risk of antimony and its compounds to organisms around the mining areas has increased, and antimony contamination studies have attracted more attention at home and abroad. To elucidate the toxic effects of contaminated soil in antimony mining areas on surrounding organisms, the effects of Sb were systematically evaluated by the soil invertebrate model organism earthworm (Eisenia fetida). The survival, antimony enrichment, total protein, antioxidant enzymes and malondialdehyde (MDA) of the earthworm were investigated by adding KSbO₆H₆ to artificial soil to simulate contaminated soil. And the biomarker response index (BRI) was used to evaluate the toxicity response of earthworms under antimony stress. The results showed that: (1) Earthworm mortality was significantly correlated with the dose effect of soil Sb concentration and exposure time, and the calculated the 56-day LC₅₀ was 4,380.37 mg/kg. (2) Limited uptake of Sb was observed, only 213 mg/kg was enriched in earthworms after 56 d exposure at the highest dose treatment 12,800 mg/kg. (3) Superoxide dismutase (SOD), peroxidase (POD) and MDA showed an inverted ‘U’ shaped change of increasing and then decreasing during 28 days of exposure, while catalase (CAT) showed an overall trend of ‘up-down-up-down’. Total protein decreased with the increase of exposure time and treatment level. (4) Among the 24 Sb-containing treatments during the exposure period, 20 treatments showed moderate or lower level of health effects, and only 4 treatments had a BRI of less than 2.5, which was considered as severe health effects. Overall, all five biomarkers were sensitive to Sb; the toxic effect of Sb on Eisenia fetida was not strong. The results of this study can provide key toxicological data for Sb contamination of soil.
- soil,
- Eisenia fetida,
- Sb,
- toxic effect,
- antioxidant enzymes,
- biomarker response index

FullText(HTML)

References(53)

References

[1]	何孟常,云影.锑矿区土壤中锑的形态及生物有效性[J].环境化学,2003,22(2):126-130. doi: 10.3321/j.issn:0254-6108.2003.02.005 HE M C,YUN Y.The speciation and bioavailability of antimony in the soils near antimony mine area[J].Environmental Chemistry,2003,22(2):126-130. doi: 10.3321/j.issn:0254-6108.2003.02.005
[2]	何孟常,万红艳.环境中锑的分布、存在形态及毒性和生物有效性[J].化学进展,2004,16(1):131-135. doi: 10.3321/j.issn:1005-281X.2004.01.020 HE M C,WAN H Y.Distribution,speciation,toxicity and bioavailability of antimony in the environment[J].Progress in Chemistry,2004,16(1):131-135. doi: 10.3321/j.issn:1005-281X.2004.01.020
[3]	BOLAN N,KUMAR M,SINGH E,et al.Antimony contamination and its risk management in complex environmental settings:a review[J].Environment International,2022,158:106908. doi: 10.1016/j.envint.2021.106908
[4]	刘灵飞,龙健,万洪富,等.贵州喀斯特山区锑冶炼厂对农业土壤污染特征的影响及风险评价[J].土壤,2013,45(6):1036-1047. doi: 10.13758/j.cnki.tr.2013.06.013 LIU L F,LONG J,WAN H F,et al.Distribution characteristics and risk assessment of heavy metals in agricultural soils in an abandoned antimony smelter in Guizhou Karst areas[J].Soils,2013,45(6):1036-1047. doi: 10.13758/j.cnki.tr.2013.06.013
[5]	QI C C,WU F C,DENG Q J,et al.Distribution and accumulation of antimony in plants in the super-large Sb deposit areas,China[J].Microchemical Journal,2011,97(1):44-51. doi: 10.1016/j.microc.2010.05.016
[6]	GUO W J,ZHANG Z Y,WANG H,et al.Exposure characteristics of antimony and coexisting arsenic from multi-path exposure in typical antimony mine area[J].Journal of Environmental Management,2021,289:112493. doi: 10.1016/j.jenvman.2021.112493
[7]	JONES R D.Survey of antimony workers:mortality 1961-1992[J].Occupational and Environmental Medicine,1994,51(11):772-776. doi: 10.1136/oem.51.11.772
[8]	ALKHAWAJAH A,LARBI E B,JAIN S,et al.Subacute toxicity of pentavalent antimony compounds in rats[J].Human & Experimental Toxicology,1992,11(4):283-288.
[9]	FENG R W,LEI L,SU J M,et al.Toxicity of different forms of antimony to rice plant:effects on root exudates,cell wall components,endogenous hormones and antioxidant system[J].Science of the Total Environment,2020,711:134589. doi: 10.1016/j.scitotenv.2019.134589
[10]	LI J H,ZHANG Z,WANG H,et al.Urban land-use impacts on composition and spatiotemporal variations in abundance and biomass of earthworm community[J].Journal of Forestry Research,2020,31(1):325-331. doi: 10.1007/s11676-018-0807-2
[11]	AINI S N,YUSNAINI S,TUNSIYAH T,et al.Minimum tillage and in situ mulch increasing the population and biomass of earthworms under mung bean cultivation on ultisol soil[J].Journal of Tropical Soils,2019,24(3):141. doi: 10.5400/jts.2019.v24i3.141-148
[12]	XIAO L,LI M H,DAI J,et al.Assessment of earthworm activity on Cu,Cd,Pb and Zn bioavailability in contaminated soils using biota to soil accumulation factor and DTPA extraction[J].Ecotoxicology and Environmental Safety,2020,195:110513. doi: 10.1016/j.ecoenv.2020.110513
[13]	CHEN Z B,HU S S.Heavy metals distribution and their bioavailability in earthworm assistant sludge treatment wetland[J].Journal of Hazardous Materials,2019,366:615-623. doi: 10.1016/j.jhazmat.2018.12.039
[14]	SHU W J,YANG Z F,XU Z N,et al.Effects of one-dimensional nanomaterial polyaniline nanorods on earthworm biomarkers and soil enzymes[J].Environmental Science and Pollution Research,2022,29(23):35217-35229. doi: 10.1007/s11356-021-18260-1
[15]	ČESYNAITĖ J,PRASPALIAUSKAS M,PEDIŠIUS N,et al.Biological assessment of contaminated shooting range soil using earthworm biomarkers[J].Ecotoxicology,2021,30(10):2024-2035. doi: 10.1007/s10646-021-02463-w
[16]	颜增光,何巧力,李发生.蚯蚓生态毒理试验在土壤污染风险评价中的应用[J].环境科学研究,2007,20(1):134-142. doi: 10.3321/j.issn:1001-6929.2007.01.026 YAN Z G,HE Q L,LI F S.The use of earthworm ecotoxicological test in risk assessment of soil contamination[J].Research of Environmental Sciences,2007,20(1):134-142. doi: 10.3321/j.issn:1001-6929.2007.01.026
[17]	郝桂玉,黄民生,徐亚同.蚯蚓及其在生态环境保护中的应用[J].环境科学研究,2004,17(3):75-77. doi: 10.3321/j.issn:1001-6929.2004.03.020 HAO G Y,HUANG M S,XU Y T.Earthworms and their application to eco-environmental protection[J].Research of Environmental Sciences,2004,17(3):75-77. doi: 10.3321/j.issn:1001-6929.2004.03.020
[18]	XU Z N,YANG Z F,SHU W J,et al.Combined toxicity of soil antimony and cadmium on earthworm Eisenia fetida:Accumulation,biomarker responses and joint effect[J].Journal of Hazardous Materials Letters,2021,2:100018. doi: 10.1016/j.hazl.2021.100018
[19]	KUPERMAN R G,CHECKAI R T,SIMINI M,et al.Toxicity benchmarks for antimony,Barium,and beryllium determined using reproduction endpoints for Folsomia candida,Eisenia fetida,and Enchytraeus crypticus[J].Environmental Toxicology and Chemistry,2006,25(3):754-762. doi: 10.1897/04-545R.1
[20]	梁淑轩,王凯,耿梦娇,等.土壤中添加Sb(Ⅲ)对赤子爱胜蚓金属硫蛋白的影响[J].环境污染和保护科学学报,2003,2(2):31-36. LIANG S X,WANG K,GENG M J,et al.Effect of adding antimony pollution on metallothionein of Eisenia fetida in soil[J].Scientific Journal of Environment Pollution and Protection,2003,2(2):31-36.
[21]	ZHONG Q Y,LI L Z,HE M C,et al.Toxicity and bioavailability of antimony to the earthworm (Eisenia fetida) in different agricultural soils[J].Environmental Pollution (Barking,Essex:1987),2021,291:118215. doi: 10.1016/j.envpol.2021.118215
[22]	XIA X Q,LIN S Y,ZHAO J,et al.Toxic responses of microorganisms to nickel exposure in farmland soil in the presence of earthworm (Eisenia fetida)[J].Chemosphere,2018,192:43-50. doi: 10.1016/j.chemosphere.2017.10.146
[23]	XING Y S,MENG X S,WANG L,et al.Effects of benzotriazole on copper accumulation and toxicity in earthworm (Eisenia fetida)[J].Journal of Hazardous Materials,2018,351:330-336. doi: 10.1016/j.jhazmat.2018.03.019
[24]	OECD.Test No.222:earthworm reproduction test (Eisenia fetida/Eisenia andrei)[M].Paris:OECD,2016.
[25]	ISO11268.Determination of acute toxicity to Eisenia/Eisenia andrei[S].London:International Organization for Standardization Provided,2015.
[26]	VANEWIJK P H,HOEKSTRA J A.Calculation of the EC₅₀ and its confidence interval when subtoxic stimulus is present[J].Ecotoxicology and Environmental Safety,1993,25(1):25-32. doi: 10.1006/eesa.1993.1003
[27]	LI X Z,WANG M E,CHEN W P,et al.Evaluation of combined toxicity of Siduron and cadmium on earthworm (Eisenia fetida) using Biomarker Response Index[J].Science of the Total Environment,2019,646:893-901. doi: 10.1016/j.scitotenv.2018.07.380
[28]	PIVA F,CIAPRINI F,ONORATI F,et al.Assessing sediment hazard through a weight of evidence approach with bioindicator organisms:a practical model to elaborate data from sediment chemistry,bioavailability,biomarkers and ecotoxicological bioassays[J].Chemosphere,2011,83(4):475-485. doi: 10.1016/j.chemosphere.2010.12.064
[29]	HAGGER J A,JONES M B,LOWE D,et al.Application of biomarkers for improving risk assessments of chemicals under the Water Framework Directive:a case study[J].Marine Pollution Bulletin,2008,56(6):1111-1118. doi: 10.1016/j.marpolbul.2008.03.040
[30]	张聪,王志新,刘新会,等.河北黄壤中铅和铬(Ⅵ)对赤子爱胜蚓的毒性效应[J].环境化学,2021,40(6):1683-1690. doi: 10.7524/j.issn.0254-6108.2020073002 ZHANG C,WANG Z X,LIU X H,et al.Toxic effects of lead and chromium(Ⅵ) on the earthworm (Eisenia fetida) in Hebei soils[J].Environmental Chemistry,2021,40(6):1683-1690. doi: 10.7524/j.issn.0254-6108.2020073002
[31]	CHEN Y J,LIU X G,YUAN S K,et al.Accumulation of epoxiconazole from soil via oleic acid-embedded cellulose acetate membranes and bioavailability evaluation in earthworms (Eisenia fetida)[J].Environmental Pollution,2022,292:118283. doi: 10.1016/j.envpol.2021.118283
[32]	WANG K,QIAO Y H,ZHANG H Q,et al.Bioaccumulation of heavy metals in earthworms from field contaminated soil in a subtropical area of China[J].Ecotoxicology and Environmental Safety,2018,148:876-883. doi: 10.1016/j.ecoenv.2017.11.058
[33]	CHO J H,TAE H J,KIM I S,et al.Melatonin alleviates asphyxial cardiac arrest-induced cerebellar Purkinje cell death by attenuation of oxidative stress[J].Experimental Neurology,2019,320:112983. doi: 10.1016/j.expneurol.2019.112983
[34]	HUANG B C,LONG J,LI J,et al.Effects of antimony contamination on bioaccumulation and gut bacterial community of earthworm Eisenia fetida[J].Journal of Hazardous Materials,2021,416:126110. doi: 10.1016/j.jhazmat.2021.126110
[35]	林祥龙,孙在金,陈卫玉,等.锑对土壤跳虫(Folsomia candida)的毒性效应[J].环境科学研究,2017,30(7):1089-1097. LIN X L,SUN Z J,CHEN W Y,et al.Toxicity effect of antimony to soil-dwelling springtail (Folsomia candida)[J].Research of Environmental Sciences,2017,30(7):1089-1097.
[36]	LIN X L,SUN Z J,ZHAO L,et al.Toxicity of exogenous antimony to the soil-dwelling springtail Folsomia candida[J].Environmental Science and Pollution Research,2019,26(6):5658-5667. doi: 10.1007/s11356-018-3727-y
[37]	LIMONTA G,MANCIA A,ABELLI L,et al.Effects of microplastics on head kidney gene expression and enzymatic biomarkers in adult zebrafish[J].Comparative Biochemistry and Physiology Toxicology & Pharmacology:CBP,2021,245:109037.
[38]	MAITY S,BANERJEE R,GOSWAMI P,et al.Oxidative stress responses of two different ecophysiological species of earthworms (Eutyphoeus waltoni and Eisenia fetida) exposed to Cd-contaminated soil[J].Chemosphere,2018,203:307-317. doi: 10.1016/j.chemosphere.2018.03.189
[39]	李井懿,童一帆,程千卉,等.海水中原油对双齿围沙蚕(Perinereis aibuhitensis)的急性毒性效应及其体内抗氧化酶活性的影响[J].环境科学研究,2018,31(11):1972-1978. LI J Y,TONG Y F,CHENG Q H,et al.Acute toxicity and the effects on antioxidant enzyme activities of polychaete Perinereis aibuhitensis exposed to crude oil in seawater[J].Research of Environmental Sciences,2018,31(11):1972-1978.
[40]	刘信勇,朱琳,黄碧捷,等.多壁碳纳米管对斑马鱼体组织内酶活性的影响[J].环境科学研究,2009,22(7):838-842. doi: 10.13198/j.res.2009.07.88.liuxy.013 LIU X Y,ZHU L,HUANG B J,et al.Effects of multi-walled carbon nanotubes on enzyme activity in tissues of zebrafish[J].Research of Environmental Sciences,2009,22(7):838-842. doi: 10.13198/j.res.2009.07.88.liuxy.013
[41]	张薇,宋玉芳,孙铁珩,等.菲和芘对蚯蚓(Eisenia fetida)细胞色素P450和抗氧化酶系的影响[J].环境化学,2007,26(2):202-206. doi: 10.3321/j.issn:0254-6108.2007.02.018 ZHANG W,SONG Y F,SUN T H,et al.Influence of phenanthrene and pyrene on cytochrome p450 and antioxidant enzymes in earthworms (Eisenia fetida)[J].Environmental Chemistry,2007,26(2):202-206. doi: 10.3321/j.issn:0254-6108.2007.02.018
[42]	毕爽,刘征涛,徐镜波,等.土壤Pb暴露下赤子爱胜蚓(Eisenia fetida)的急性毒性和氧化应激反应[J].环境科学研究,2014,27(2):196-202. BI S,LIU Z T,XU J B,et al.Acute toxicity and oxidation stress response of Eisenia fetida under lead exposure in soil[J].Research of Environmental Sciences,2014,27(2):196-202.
[43]	杜宇,王应军,武阳,等.镧胁迫对蚯蚓几种重要酶活性的影响[J].中国稀土学报,2014,32(1):84-93. DU Y,WANG Y J,WU Y,et al.Effects of lanthanum on several important enzyme activities of earthworm[J].Journal of the Chinese Society of Rare Earths,2014,32(1):84-93.
[44]	ZHOU D X,NING Y C,WANG B,et al.Study on the influential factors of Cd²⁺ on the earthworm Eisenia fetida in oxidative stress based on factor analysis approach[J].Chemosphere,2016,157:181-189. doi: 10.1016/j.chemosphere.2016.05.045
[45]	D'AGOSTINO A,GIANNONE D.Comparing alternative predictors based on large-panel factor models[J].Oxford Bulletin of Economics and Statistics,2012,74(2):306-326. doi: 10.1111/j.1468-0084.2011.00642.x
[46]	SONG X H,WANG K,GUO L L,et al.Effects of catalase and ascorbate peroxidase on the root growth of rice under cadmium stress[J].Agricultural Science and Technology Hunan,2011,12:1256-1259.
[47]	王文银,高小刚,司晓林,等.外源钙盐对盐胁迫下沙拐枣渗透调节和膜脂过氧化的影响[J].环境科学研究,2017,30(8):1230-1237. doi: 10.13198/j.issn.1001-6929.2017.02.58 WANG W Y,GAO X G,SI X L,et al.Effects of exogenous calcium on osmotic adjustment and peroxidation of Calligonum mongolicum membrane under salt stress[J].Research of Environmental Sciences,2017,30(8):1230-1237. doi: 10.13198/j.issn.1001-6929.2017.02.58
[48]	王婉华,陈丽红,张聪,等.铬(Ⅵ)老化对赤子爱胜蚓抗氧化酶活性的影响[J].环境科学研究,2015,28(11):1687-1692. doi: 10.13198/j.issn.1001-6929.2015.11.05 WANG W H,CHEN L H,ZHANG C,et al.Effects of aged chromium(Ⅵ) on activities of antioxidant enzymes of Eisenia fetida[J].Research of Environmental Sciences,2015,28(11):1687-1692. doi: 10.13198/j.issn.1001-6929.2015.11.05
[49]	FOSCOLOU A,CRITSELIS E,TYROVOLAS S,et al.Plant and animal protein consumption,cardiometabolic risk and healthy aging:attica and medis epidemiological studies[J].Atherosclerosis,2020,315:e100-e101.
[50]	MULLEN P,ABBOTT J A,WELLMAN T,et al.Neuropathy-associated histidyl-tRNA synthetase variants attenuate protein synthesis in vitro and disrupt axon outgrowth in developing zebrafish[J].The FEBS Journal,2021,288(1):142-159. doi: 10.1111/febs.15449
[51]	BROEG K,WESTERNHAGEN H V,ZANDER S,et al.The ‘bioeffect assessment index’ (BAI):a concept for the quantification of effects of marine pollution by an integrated biomarker approach[J].Marine Pollution Bulletin,2005,50(5):495-503. doi: 10.1016/j.marpolbul.2005.02.042
[52]	AARAB N,CHAMPEAU O,MORA P,et al.Scoring approach based on fish biomarkers applied to French River monitoring[J].Biomarkers,2004,9(3):258-270. doi: 10.1080/13547500400015626
[53]	NARBONNE J F,AARAB N,CLÉRANDEAU C,et al.Scale of classification based on biochemical markers in mussels:application to pollution monitoring in Mediterranean coasts and temporal trends[J].Biomarkers,2005,10(1):58-71. doi: 10.1080/13547500500071339

Relative Articles

Supplements(0)

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Figures(4) / Tables(2)

Get Citation

PDF

XML

Article Metrics

Article views (168) PDF downloads(59)

Toxicity of Soil Antimony to Eisenia fetida at Individual and Cellular Levels

doi: 10.13198/j.issn.1001-6929.2022.11.10

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Toxicity of Soil Antimony to Eisenia fetida at Individual and Cellular Levels

doi: 10.13198/j.issn.1001-6929.2022.11.10

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Export File

Citation

Format

Content