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稻田土壤β-1,4-葡萄糖苷酶活性对温度变化的响应特征
周璞,魏亮,魏晓梦,祝贞科,袁红朝,李巧云,吴金水,等
作者单位E-mail
周璞 湖南农业大学生物科学技术学院 zplpcapb@163.com 
魏亮 中国科学院亚热带农业生态研究所  
魏晓梦 中国科学院亚热带农业生态研究所  
祝贞科 中国科学院亚热带农业生态研究所  
袁红朝 中国科学院亚热带农业生态研究所  
李巧云 湖南农业大学生物科学技术学院 liqiaoyun@hunan.edu.cn 
吴金水 中国科学院亚热带农业生态研究所  
摘要:
温度是土壤酶活性的关键非生物影响因子,调控着土壤物质周转过程。为了探究温度变化对稻田土壤有机质周转及其关键胞外酶活性的影响,该研究设计室内培养实验,分别在5℃、15℃、25℃和35℃条件下测定亚热带稻田土壤β-1,4-葡萄糖苷酶活性(BG),探究温度对土壤胞外酶活性及其与碳氮转化过程的影响特征。结果表明,稻田土壤中可利用态碳(DOC)、铵态氮(NH4+-N)和土壤微生物生物量含量在5℃~25℃条件下随着培养时间的增加而降低。BG最大酶活性在15 天时达到306.6~437.8 nmol/g/h,并随温度的增加表现为先增后减,在第3和75天时,25℃条件下BG酶活性最大为184.5~404.6 nmol/g/h。土壤酶活性的温度敏感性在15℃升至25℃时表现出正响应(Q10=1.5),而在5℃~15℃和25℃~35℃时Q10值小于1,表现为消除效应。土壤酶活性的变化是多因素共同影响的结果,温度升高增加微生物活性,具体来讲,温度改变土壤DOC和NH4+-N含量,影响土壤微生物量进而影响土壤BG酶活性。研究典型稻田土壤碳氮转化关键酶活性的温度响应特征,对于理解季节性土壤有机质周转规律和保持土壤肥力具有重要的理论指导意义。
关键词:  稻田土壤  土壤温度  可利用态养分含量  β-1,4-葡萄糖苷酶活性  温度敏感性
DOI:
分类号:
基金项目:国家自然科学基金(41501321);湖南省自然科学基金项目(2016JJ3132);农业部长江中游平原农业环境重点实验室开放基金
Responses of β-1,4-Glucosidase Activity to Temperature Changes in Paddy Soil
Zhou Pu,Wei Liang,Li Qiao-yun,吴金水,et al
Abstract:
Temperature, as the key biotic factor, affects the soil enzyme activity and regulates soil carbon (C) and nitrogen (N) cycling. In order to clarify the response of temperature changes to the β-1,4-glucosidase (BG) enzyme which mainly involves in soil C and N turnover, an incubation experiment was conducted at 5, 15, 25 and 35 ℃, respectively. The results showed that soil dissolved organic carbon (DOC), Ammonium nitrogen (NH4+-N) and soil microbial biomasses were decreased over time at 5 and 25 ℃. The maximal enzyme activity potential of glucosidase was ranged in 306.6~437.8 nmol/g/h at 15 days after incubation. On the contrary, the highest activity of BG was 184.5~404.6 nmol/g/h at 25 ℃on D3 and D75, and the high temperature sensitivity of BG was observed at 15- 25 ℃ (Q10 = 1.5), while the cancelling effects of enzyme activity(Q10< 1) was observed both at 5-15 ℃ and 25-35 ℃, respectively. The change of soil enzyme activity was effected by multi-factors, and the increase of temperature stimulated soil microbial activities. As a result, temperature changed DOC and NH4+-N content and exaggerated soil microbial biomass triggering changes in glucosidase activity. This study further quantified the temperature sensitivity of key enzyme activity involved in soil C turnover and its correlation with the soil physical and chemical factors, which was beneficial to revealing the C cycle of paddy soil and its regulation mechanism.
Key words:  paddy soil, soil temperature, soil available nutrient content, β-1,4-glucosidase, temperature sensitivity