引用本文:王建超,郝瑞霞,孟成成,任晓克,等.3DBER-S反硝化脱氮性能及其菌群特征[J].环境科学研究,2015,28(2):310-317.
WANG Jianchao,HAO Ruixia,MENG Chengcheng,REN Xiaoke,et al.Study on Performance and Bacterial Community of Coupling 3-Dimensional Biofilm-Electrode with Sulfur Autotrophic Denitrification[J].Reserrch of Environmental Science,2015,28(2):310-317.]
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3DBER-S反硝化脱氮性能及其菌群特征
王建超, 郝瑞霞, 孟成成, 任晓克
北京工业大学建筑工程学院, 北京市水质科学与水环境恢复工程重点实验室, 北京 100124
摘要:
针对污水处理厂尾水TN去除问题,采用16S rDNA克隆文库法,探究了3DBER-S(三维电极生物膜耦合硫自养脱氮工艺)的强化脱氮机制及其菌群特征. 结果表明,I(电流)和HRT(水力停留时间)对3DEBR-S中氢自养和硫自养反硝化作用所占比例的影响较大,但对脱氮效率影响不显著. 当进水C/N〔ρ(CODCr)/ρ(TN)〕为1、ρ(NO3--N)为35 mg/L、I为300 mA、HRT为4 h时,NO3--N和TN去除率可分别稳定在80%和74%以上. 16S rDNA克隆文库结果显示,反应器中β变形菌纲为优势菌群,占47.89%〔以OUT(操作单元)计〕. 在β变形菌纲中,与具有反硝化功能的陶厄氏菌属(Thauera)相似的细菌所占比例最大,为52.94%;与可分别利用硫和氢为电子供体进行反硝化脱氮的硫杆菌属(Thiobacillus)和食酸菌属(Acidovorax)相似的细菌分别占17.65%和14.71%. 3DBER-S中存在异养联合氢自养和硫自养反硝化协同去除硝酸盐氮的作用,可为反硝化脱氮提供充足的电子供体,节约了有机碳源消耗,并保证了稳定高效的脱氮效果.
关键词:  三维电极生物膜反应器(3DBER-S)  氢自养反硝化  硫自养反硝化  低C/N  16S rDNA克隆文库
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基金项目:国家自然科学基金项目(51378028)
Study on Performance and Bacterial Community of Coupling 3-Dimensional Biofilm-Electrode with Sulfur Autotrophic Denitrification
WANG Jianchao, HAO Ruixia, MENG Chengcheng, REN Xiaoke
Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China
Abstract:
To strengthen the removal TN for wastewater treatment plant (WWTP) effluent, the performance and bacterial community of a coupling 3-dimensional biofilm-electrode with sulfur autotrophic denitrification technology (3DBER-S) was investigated under different operating condition experiments and 16S rDNA clone library. It was found that the influences of electric current and hydraulic retention time (HRT) were obvious on the proportion of hydrogen and sulfur autotrophic denitrification process in 3DBER-S, but there was no significant impact on the denitrification performance. When CODCr/TN (C/N) was 1 and NO3--N concentration was 35 mg/L in the influent, NO3--N and TN removal rates were above 80% and 74% respectively under conditions of electric current of 300mA and HRT of 4 h. The analysis of 16S rDNA clone library showed that the dominant populations were β-Proteobacteria (47.89%) in the biofilm of 3DBER-S. 52.94% of the β-Proteobacteria consisted of a bacteria similar to Thauera that could perform denitrification. Moreover, the bacteria similar to Thiobacillus and Acidovorax accounted for 17.65% and 14.71% respectively of the β-Proteobacteria, which can use sulfur and hydrogen as an electron donor for denitrification respectively. There is a mutual complementation among heterotrophic, hydrogen and sulfur autotrophic denitrification processes, which supply 3DBER-S with enough electron donors. Then the stable and high-performance nitrogen removal can be maintained under different operating conditions, even if the carbon source of the influent is insufficient.
Key words:  3-dimensional biofilm-electrode reactor (3DBER-S)  hydrogen autotrophic denitrification  sulfur autotrophic denitrification  low C/N  16S rDNA clone library