引用本文:聂小琴,董发勤,丁德馨,等.U(Ⅵ)胁迫下碎米莎草体内抗氧化系统响应特征[J].环境科学研究,2015,28(4):621-628.
NIE Xiaoqin,DONG Faqin,DING Dexin,et al.The Responses Characteristics of the Antioxidant System of Cyperus iria L. to Uranium Stress[J].Research of Environmental Sciences,2015,28(4):621-628.]
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 1931次   下载 4361 本文二维码信息
码上扫一扫!
分享到: 微信 更多
U(Ⅵ)胁迫下碎米莎草体内抗氧化系统响应特征
聂小琴1, 董发勤1, 丁德馨2, 刘 宁3, 张 东4, 李广悦2, 刘明学1
1.西南科技大学, 核废物与环境安全国防重点学科实验室, 四川 绵阳 621010 ;2.南华大学, 铀矿冶生物技术国防重点学科实验室, 湖南 衡阳 421001 ;3.四川大学原子核科学技术研究所, 辐射物理及技术教育部重点实验室, 四川 成都 610064 ;4.中国工程物理研究院核物理与化学研究所, 四川 绵阳 621900
摘要:
采用水培方式研究了铀尾矿库土著优势植物——碎米莎草不同生长期各部位在不同质量浓度铀〔U(Ⅵ),记为U〕溶液胁迫下w(SP)(SP为可溶性蛋白)、CMDA(丙二醛含量)以及SOD(superoxide dismutase,超氧化物歧化酶)、POD(peroxidase,过氧化物酶)、CAT(catalase,过氧化氢酶)、ATPase(三磷酸腺苷酶)活性的变化. 结果表明,ρ(U)≤250 mg/L时,碎米莎草各部位SOD、POD、CAT活性在整个生长阶段均受到显著诱导(P<0.05),而CMDA、ATPase活性与对照组相比无显著差异(P>0.05);ρ(U)≥750 mg/L时,SOD、POD和CAT活性与对照组相比显著降低(P<0.05);ρ(U)达到1 000 mg/L以上时,与对照组相比,降低趋势更显著(P<0.01),而ATPase活性受到显著诱导(P<0.05),CMDA在幼苗期显著增加(P<0.05),膜脂过氧化损伤程度严重. 表明碎米莎草对铀胁迫的生理响应中,以根系SOD、CMDA的响应最为敏感.
关键词:  碎米莎草  U(Ⅵ)  胁迫  抗氧化系统
DOI:
分类号:
基金项目:国家自然科学基金委-中国工程物理研究院联合基金项目(11176028);国家重点基础研究发展计划(973)项目(2014CB846003);核废物与环境安全国防重点学科实验室开放基金项目(12zxnp02).
The Responses Characteristics of the Antioxidant System of Cyperus iria L. to Uranium Stress
NIE Xiaoqin1,2,3, DONG Faqin1, DING Dexin2, LIU Ning3, ZHANG Dong4, LI Guangyue2, LIU Mingxue1
1.Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Southwest University of Science and Technology, Mianyang 621010, China ;2.Fundamental Science on Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China ;3.Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China ;4.Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang 621900, China
Abstract:
Abstract:The effects of the contents of soluble protein and malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and adenosine triphosphatase (ATPase) induced by uranium toxicity to Cyperus iria L. at different growth stages and positions were investigated. The results indicated that the activities of SOD, POD and CAT increased significantly (P<0.05), but there was no significant difference in the content of MDA and the activities of ATPase between the control group and the group treated by low concentration of uranium (≤250 mg/L). However, the activities of SOD, POD and CAT decreased significantly (P<0.05) as the concentration of the uranium reached up to 750 mg/L, while the activities of ATPase, the contents of MDA and soluble protein increased, which indicated serious increases of lipid peroxidation as the concentration of uranium reached 1000 mg/L (P<0.05). Thus, the activities of SOD and the contents of MDA of the root part could characterize the toxicity response of C. iria to uranium.
Key words:  Cyperus iria L.  uranium  stress  antioxidant system