引用本文:陈 斌,杨天学,耿向永,等.玉米秸秆干式厌氧发酵乙酸产生规律及其对发酵的影响[J].环境科学研究,2015,28(4):647-652.
CHEN Bin,YANG Tianxue,GENG Xiangyong,et al.Effects of Acetic Acid Production on Dry Fermentation Systems with Corn Stalk[J].Reserrch of Environmental Science,2015,28(4):647-652.]
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玉米秸秆干式厌氧发酵乙酸产生规律及其对发酵的影响
陈 斌1,2, 杨天学2, 耿向永3, 魏自民1, 席北斗2, 赵 越1, 李 雪1
1.东北农业大学生命科学学院, 黑龙江 哈尔滨 150030 ;2.中国环境科学研究院, 环境基准与风险评估国家重点实验室, 北京 100012 ;3.西藏自治区扎嚢县扎其乡农牧站, 西藏 扎嚢 850800
摘要:
为解决玉米秸秆干式厌氧发酵系统因乙酸积累而致发酵失败、工程运行不稳定等问题,利用气相色谱仪对玉米秸秆干式厌氧发酵过程中w(乙酸)和w(CH4)进行检测,结合相关性分析和回归分析等方法,研究了乙酸动态产生趋势、w(TS)(TS为总固体)与w(乙酸)的相关性,以及w(乙酸)对CH4产生的作用机制. 结果表明:发酵过程中w(乙酸)先增后降,w(TS)由20%增至30%,w(乙酸)增加的持续时间由5 d延至15 d. Pearson相关性分析结果表明,w(TS)与w(乙酸) 呈极显著负相关(R<-0.979,P<0.01). 一元线性回归分析得到拟合方程y=-1 214.8w(TS)+668.2,由斜率(-1 214.8)可知,w(TS)对产乙酸过程具有较明显的抑制作用. 玉米秸秆干式厌氧发酵系统内w(乙酸)为160~451 mg/g,随着w(乙酸)的增加,底物CH4产率经历上升、稳定、下降后再稳定和下降4个阶段,其中w(乙酸)为212~312 mg/g时,底物CH4产率(大于120 m3/t,以秸秆干质量计)最大,其次为w(乙酸)在>312~410 mg/g时的80~120 m3/t.
关键词:  干式厌氧发酵  乙酸  CH4  作用机制  玉米秸秆
DOI:
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基金项目:国家自然科学基金项目(51178090);国家“十二五”科技支撑计划项目(2012BAJ21B07)
Effects of Acetic Acid Production on Dry Fermentation Systems with Corn Stalk
CHEN Bin1,2, YANG Tianxue2, GENG Xiangyong3, WEI Zimin1, XI Beidou2, ZHAO Yue1, LI Xue1
1.College of Life Sciences, Northeast Agricultural University, Harbin 150030, China ;2.Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China ;3.The Agricultural and Animal Husbandry Station of Zhaqi Township, Zhanang 850800, China
Abstract:
Abstract:In order to solve the problems of fermentation failure and unstable operation caused by acetic acid accumulation in the dry anaerobic fermentation system, gas chromatography was used to determine the contents of acetic acid and CH4 during dry anaerobic fermentation with corn stalks. Correlations between contentes of TS, acetic acid and the dynamic tent of acetic acid production were studied by correlation analysis and regression analysis. The effect mechanism of content of acetic acid on CH4 production was also investigated. The results showed that content of acetic acid increased first and then decreased during the dry anaerobic fermentation. With content of TS increase from 20% to 30%, the duration of content of acetic acid increase extended from 5 to 15 days. Pearson correlation analysis indicates a significant negative correlation between contents of TS and acetic acid (R<-0.979, P<0.01). The equation, y=-1 214.8w(TS)+668.2, was obtained by unitary linearity regression analysis; the equation suggested that the production of acetic acid was clearly restrained by TS. During the dry anaerobic fermentation of corn stalks, content of acetic acid system was 160-451 mg/g. With the increase of content of acetic acid, the production of CH4(TVS) increased initially and then remained stable, then finally decreased. The maximum production was more than 120 m3/t with the content of acetic acid ranging from 212 to 312 mg/g, while the production of CH4 achieved 80-120 m3/t with the content of acetic acid ranging from 312 to 410 mg/g.
Key words:  dry anaerobic fermentation  acetic acid  methane  effect mechanism  corn stalks